A new data acquisition and processing system for testing vehicle's braking performance which has the function of storage, real time display and analysis is designed and implemented based on Visual C++ and combined with the multi-function data acquisition card PCI2013. In order to improve the efficiency of the system, we have used multi-thread and multi-level buffer techniques to develop it. The preliminary test results indicate that the system can show the braking performance parameters such as the brake oil pressure, stop time, braking distance, braking torque real time, and draw the corresponding test and analysis curve. So it has been proved that the system can realize real time test, data processing and analysis, test results output and other functions automatically, quickly and accurately and has high feasibility and reliability from the preliminary test results.