Development of High-Performance and Rapid Immunoassay for Model Food Allergen Lysozyme Using Antibody-Conjugated Bacterial Magnetic Particles and Fully Automated System

2001 ◽  
pp. 109-116
Author(s):  
Reiko Sato ◽  
Haruko Takeyama ◽  
Tsuyoshi Tanaka ◽  
Tadashi Matsunaga
Keyword(s):  
Food Allergen ◽  
High Performance ◽  
Magnetic Particles ◽  
Automated System ◽  
Model Food ◽  
Bacterial Magnetic Particles ◽  
Fully Automated System

Fully Automated System for Aerobic or Anaerobic Sampling of Duodenal Digesta in Sheep or Cattle Equipped with Duodenal Reentrant Cannulas1

1985 ◽  
Vol 60 (5) ◽  
pp. 1359-1366 ◽  
Author(s):  
M. Ivan ◽  
D. J. Buckley ◽  
G. St. Amour ◽  
C. F. Nicholls ◽  
D. M. Veira
Keyword(s):  
Automated System ◽  
Fully Automated System

scholarly journals Evaluation of a New Immunochromatography Technology Test (LDBio Diagnostics) To Detect Toxoplasma IgG and IgM: Comparison with the Routine Architect Technique

2017 ◽  
Vol 55 (12) ◽  
pp. 3395-3404 ◽  
Author(s):  
Caroline Mahinc ◽  
Pierre Flori ◽  
Edouard Delaunay ◽  
Cécile Guillerme ◽  
Sana Charaoui ◽  
...  
Keyword(s):  
Western Blot ◽  
Sensitivity And Specificity ◽  
Blot Analysis ◽  
False Positive ◽  
Automated System ◽  
University Hospitals ◽  
Content Type ◽  
Automated Screening ◽  
Commercial Kits ◽  
Fully Automated System

ABSTRACTA study comparing the ICT (immunochromatography technology)ToxoplasmaIgG and IgM rapid diagnostic test (LDBio Diagnostics, France) with a fully automated system, Architect, was performed on samples from university hospitals of Marseille and Saint-Etienne. A total of 767 prospective sera and 235 selected sera were collected. The panels were selected to test various IgG and IgM parameters. The reference technique,ToxoplasmaIgGII Western blot analysis (LDBio Diagnostics), was used to confirm the IgG results, and commercial kits Platelia Toxo IgM (Bio-Rad) and Toxo-ISAgA (bioMérieux) were used in Saint-Etienne and Marseille, respectively, as the IgM reference techniques. Sensitivity and specificity of the ICT and the Architect IgG assays were compared using a prospective panel. Sensitivity was 100% for the ICT test and 92.1% for Architect (cutoff at 1.6 IU/ml). The low-IgG-titer serum results confirmed that ICT sensitivity was superior to that of Architect. Specificity was 98.7% (ICT) and 99.8% (Architect IgG). The ICT test is also useful for detecting IgM without IgG and is both sensitive (100%) and specific (100%), as it can distinguish nonspecific IgM from specificToxoplasmaIgM. In comparison, IgM sensitivity and specificity on Architect are 96.1% and 99.6%, respectively (cutoff at 0.5 arbitrary units [AU]/ml). To conclude, this new test overcomes the limitations of automated screening techniques, which are not sensitive enough for IgG and lack specificity for IgM (rare IgM false-positive cases).

scholarly journals Development of a Germ-Free Murine Model for Prediction of Food Allergen Potency: Preliminary Studies Using Peanut Ara h1 and Ara h2 As Model Food Allergens

2016 ◽  
Vol 137 (2) ◽  
pp. AB406
Author(s):  
Nathan L. Marsteller ◽  
Kwame Andoh-Kumi ◽  
Stef J. Koppelman ◽  
Richard E. Goodman ◽  
Joe L. Baumert
Keyword(s):  
Murine Model ◽  
Food Allergen ◽  
Food Allergens ◽  
Germ Free ◽  
Ara H1 ◽  
Model Food

scholarly journals Assembly of G Protein-Coupled Receptors onto Nanosized Bacterial Magnetic Particles Using Mms16 as an Anchor Molecule

2004 ◽  
Vol 70 (5) ◽  
pp. 2880-2885 ◽  
Author(s):  
Tomoko Yoshino ◽  
Masayoshi Takahashi ◽  
Haruko Takeyama ◽  
Yoshiko Okamura ◽  
Fukuichi Kato ◽  
...  
Keyword(s):  
G Protein ◽  
Magnetic Particles ◽  
Lipid Membrane ◽  
Specific Protein ◽  
G Protein Coupled Receptors ◽  
Native Conformation ◽  
Wide Range ◽  
Bacterial Magnetic Particles ◽  
Magnetospirillum Magneticum ◽  
G Protein Coupled

ABSTRACT G protein-coupled receptors (GPCRs) play a central role in a wide range of biological processes and are prime targets for drug discovery. GPCRs have large hydrophobic domains, and therefore purification of GPCRs from cells is frequently time-consuming and typically results in loss of native conformation. In this work, GPCRs have been successfully assembled into the lipid membrane of nanosized bacterial magnetic particles (BMPs) produced by the magnetic bacterium Magnetospirillum magneticum AMB-1. A BMP-specific protein, Mms16, was used as an anchor molecule, and localization of heterologous Mms16 on BMPs was confirmed by luciferase fusion studies. Stable luminescence was obtained from BMPs bearing Mms16 fused with luciferase at the C-terminal region. D1 dopamine receptor (D1R), a GPCR, was also efficiently assembled onto BMPs by using Mms16 as an anchor molecule. D1R-BMP complexes were simply extracted by magnetic separation from ruptured AMB-1 transformants. After washing, the complexes were ready to use for analysis. This system conveniently refines the native conformation of GPCRs without the need for detergent solubilization, purification, and reconstitution after cell disruption.

Magnetically tightened form-stable phase change materials with modular assembly and geometric conformality features

2021 ◽  
Author(s):  
Yongyu Lu ◽  
Dehai Yu ◽  
Haoxuan Dong ◽  
Jinran Lv ◽  
Lichen Wang ◽  
...  
Keyword(s):  
Phase Change ◽  
High Performance ◽  
Magnetic Particles ◽  
Phase Change Materials ◽  
Stable Phase ◽  
Free Standing ◽  
Modular Assembly ◽  
Significant Step ◽  
Dynamic Assembly ◽  
Surrounding Space

Abstract Recently, phase change materials (PCMs) have attracted significant attention due to their promising applications in many fields like solar energy and chip cooling. However, the present PCMs seriously suffer inevitable leakage and low thermal conduction. Magnetism can produce invisible field effects in the surrounding space. If there exist magnetic particles within this region, the effects will act on them emerging various fascinating phenomena. Inspired by this, we introduce hard magnetic particles (which can keep the effect after removing the magnetic field) to PCMs synthesizing an unprecedented magnetically tightened form-stable PCMs (MTPCMs), achieving multifunctions of leakage-proof, dynamic assembly and morphological reconfiguration, superior high thermal (increasing of 1400%~1600%) and electrical (>104 S/m) conductivity, and prominent compressive strength. Novel free-standing temperature control and high-performance thermal and electric conversion systems based on MTPCMs are furthermore developed. This work is a significant step toward exploiting a smart PCM for electronics and low-temperature energy storage.

scholarly journals Fully automated cognitive screening tool based on assessment of speech and language

2020 ◽  
Vol 92 (1) ◽  
pp. 12-15
Author(s):  
Ronan Peter Daniel O'Malley ◽  
Bahman Mirheidari ◽  
Kirsty Harkness ◽  
Markus Reuber ◽  
Annalena Venneri ◽  
...  
Keyword(s):  
Cognitive Disorders ◽  
Cognitive Disorder ◽  
Current Data ◽  
Automated System ◽  
Cognitive Screening ◽  
Machine Learning Classification ◽  
Speaker Segmentation ◽  
Memory Clinics ◽  
Memory Concerns ◽  
Fully Automated System

IntroductionRecent years have seen an almost sevenfold rise in referrals to specialist memory clinics. This has been associated with an increased proportion of patients referred with functional cognitive disorder (FCD), that is, non-progressive cognitive complaints. These patients are likely to benefit from a range of interventions (eg, psychotherapy) distinct from the requirements of patients with neurodegenerative cognitive disorders. We have developed a fully automated system, ‘CognoSpeak’, which enables risk stratification at the primary–secondary care interface and ongoing monitoring of patients with memory concerns.MethodsWe recruited 15 participants to each of four groups: Alzheimer’s disease (AD), mild cognitive impairment (MCI), FCD and healthy controls. Participants responded to 12 questions posed by a computer-presented talking head. Automatic analysis of the audio and speech data involved speaker segmentation, automatic speech recognition and machine learning classification.ResultsCognoSpeak could distinguish between participants in the AD or MCI groups and those in the FCD or healthy control groups with a sensitivity of 86.7%. Patients with MCI were identified with a sensitivity of 80%.DiscussionOur fully automated system achieved levels of accuracy comparable to currently available, manually administered assessments. Greater accuracy should be achievable through further system training with a greater number of users, the inclusion of verbal fluency tasks and repeat assessments. The current data supports CognoSpeak’s promise as a screening and monitoring tool for patients with MCI. Pending confirmation of these findings, it may allow clinicians to offer patients at low risk of dementia earlier reassurance and relieve pressures on specialist memory services.

A fully automated system using a microcomputer for the hypertensive activity assays of drugs in the anesthetized rat

1987 ◽  
Vol 18 (1) ◽  
pp. 69-80 ◽  
Author(s):  
Francis Devaux ◽  
Patrick Herbelin ◽  
Françoise Acezat ◽  
Christine Goizel
Keyword(s):  
Automated System ◽  
Hypertensive Activity ◽  
Fully Automated System
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