In Vitro Degradation of Insoluble Lignin in Aqueous Media by Lignin Peroxidase and Manganese Peroxidase

Author(s):  
David N. Thompson ◽  
Bonnie R. Hames ◽  
C. A. Reddy ◽  
Hans E. Grethlein
1998 ◽  
Vol 70-72 (1) ◽  
pp. 967-982 ◽  
Author(s):  
David N. Thompson ◽  
Bonnie R. Hames ◽  
C. A. Reddy ◽  
Hans E. Grethlein

2001 ◽  
Vol 75 (3) ◽  
pp. 362-368 ◽  
Author(s):  
M. T. Moreira ◽  
C. Palma ◽  
I. Mielgo ◽  
G. Feijoo ◽  
J. M. Lema

2005 ◽  
Vol 43 (1) ◽  
pp. 13-19 ◽  
Author(s):  
Devendra S. Patel ◽  
Rajendra K. Aithal ◽  
Gopal Krishna ◽  
Yuri M. Lvov ◽  
Ming Tien ◽  
...  

Author(s):  
Yalavarthi Nagaraju ◽  
. Mahadevaswamy ◽  
S. B. Gowder ◽  
S. Triveni

The most prevalent aromatic polymer in nature is lignin, produced by higher plants and thought to make up 30-35 percent of the non-fossil organic carbon on the planet. Lignin hydrolyzing enzymes such as lignin peroxidase, laccase, manganese peroxidase, and others produce a variety of aromatic monomers, including ferulic and vanillic acids. However, very little research has been done on the role of microbes in lignin degradation. In the present work, we have isolated 25 ligninolytic bacteria and 25 ligninolytic fungi from organic soils of Koppal, Raichur districts of Karnataka. The bacterial isolates were identified as Pseudomonas putida, Bacillus subtilis, based on biochemical tests, and fungi were identified as Aspergillus niger, Trichoderma viridae, Phanerochaete chrysosporium and Pleurotus ostreatus based on morphological characters. The ligninolytic activity of bacterial isolates was high when compared to fungal isolates. All the isolates produced detectable amounts of lignin peroxidase, manganese peroxidase, and laccase under in vitro conditions. In dye decolorization test, fungal isolates KGST-1, KGST-2, and KKSP could decolorize Ramazol Brilliant Blue R and Congo red.


2007 ◽  
Vol 342-343 ◽  
pp. 713-716 ◽  
Author(s):  
Naoki Nakajima ◽  
Hajime Sugai ◽  
Sadami Tsutsumi ◽  
Suong Hyu Hyon

To improve the conventional and commercially-available medical adhesives such as cyanoacrylate, aldehyde-based, and fibrin glue, new bioadhesive has been prepared using medical and food additives as starting materials. Aldehyde groups could be easily introduced in dextran in the presence of sodium periodate in aqueous media, and the extent of the introduction could also be controlled. In vitro degradation speed of the hydrogel prepared by mixing of aldehyded dextran with ε-poly(L-lysine) at 37oC significantly varied by acetic anhydride concentration added to ε-poly(L-lysine) from < 5h to > 5 weeks. Bonding strength of the glue was 4 times higher than that of commercial fibrin glue and almost no cytotoxicity was observed, suggesting the development of novel self-degradable bioadhesive.


2001 ◽  
Vol 8 (6) ◽  
pp. 423-428 ◽  
Author(s):  
Krisztina Jost ◽  
Jozsef Varga ◽  
Botond Pence ◽  
Marta Zarandi

Materials ◽  
2019 ◽  
Vol 12 (2) ◽  
pp. 295 ◽  
Author(s):  
Sébastien Champagne ◽  
Ehsan Mostaed ◽  
Fariba Safizadeh ◽  
Edward Ghali ◽  
Maurizio Vedani ◽  
...  

Absorbable metals have potential for making in-demand rigid temporary stents for the treatment of urinary tract obstruction, where polymers have reached their limits. In this work, in vitro degradation behavior of absorbable zinc alloys in artificial urine was studied using electrochemical methods and advanced surface characterization techniques with a comparison to a magnesium alloy. The results showed that pure zinc and its alloys (Zn–0.5Mg, Zn–1Mg, Zn–0.5Al) exhibited slower corrosion than pure magnesium and an Mg–2Zn–1Mn alloy. The corrosion layer was composed mostly of hydroxide, carbonate, and phosphate, without calcium content for the zinc group. Among all tested metals, the Zn–0.5Al alloy exhibited a uniform corrosion layer with low affinity with the ions in artificial urine.


Molecules ◽  
2021 ◽  
Vol 26 (3) ◽  
pp. 592 ◽  
Author(s):  
Abraham M. Abraham ◽  
Reem M. Alnemari ◽  
Jana Brüßler ◽  
Cornelia M. Keck

Antioxidants are recommended to prevent and treat oxidative stress diseases. Plants are a balanced source of natural antioxidants, but the poor solubility of plant active molecules in aqueous media can be a problem for the formulation of pharmaceutical products. The potential of PlantCrystal technology is known to improve the extraction efficacy and antioxidant capacity (AOC) of different plants. However, it is not yet proved for plant waste. Black tea (BT) infusion is consumed worldwide and thus a huge amount of waste occurs as a result. Therefore, BT waste was recycled into PlantCrystals using small-scale bead milling. Their characteristics were compared with the bulk-materials and tea infusion, including particle size and antioxidant capacity (AOC) in-vitro. Waste PlantCrystals possessed a size of about 280 nm. Their AOC increased with decreasing size according to the DPPH (1,1-diphenyl-2-picrylhydrazyl) and ORAC (oxygen radical absorbance capacity) assays. The AOC of the waste increased about nine-fold upon nanonization, leading to a significantly higher AOC than the bulk-waste and showed no significant difference to the infusion and the used standard according to DPPH assay. Based on the results, it is confirmed that the PlantCrystal technology represents a natural, cost-effective plant-waste recycling method and presents an alternative source of antioxidant phenolic compounds.


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