Rapid and Specific Detection Methods for Plant Mycoplasmas

1988 ◽  
pp. 77-101 ◽  
Author(s):  
C. Hiruki
Keyword(s):  
Detection Methods ◽  
Specific Detection

Back-trajectory modelling and DNA-based species-specific detection methods allow tracking of fungal spore transport in air masses

2016 ◽  
Vol 571 ◽  
pp. 658-669 ◽  
Author(s):  
Agnieszka Grinn-Gofroń ◽  
Magdalena Sadyś ◽  
Joanna Kaczmarek ◽  
Aleksandra Bednarz ◽  
Sylwia Pawłowska ◽  
...  
Keyword(s):  
Fungal Spore ◽  
Detection Methods ◽  
Back Trajectory ◽  
Specific Detection ◽  
Air Masses ◽  
Species Specific ◽  
Trajectory Modelling

scholarly journals Rapid detection of M. bovis and M. avium in cytological smears and tissue sections by PNA-FISH

2020 ◽  
Author(s):  
Rabyia Javed ◽  
Deepti Narang ◽  
Kuldip Gupta ◽  
Siddartha Deshmukh ◽  
Mudit Chandra
Keyword(s):  
Bovine Tuberculosis ◽  
Peptide Nucleic Acid ◽  
Rapid Detection ◽  
Detection Methods ◽  
Post Mortem ◽  
Specific Detection ◽  
Tissue Sections ◽  
Formamide Concentration ◽  
Pna Fish

Abstract Background: Bovine Tuberculosis is globally the paramount cause of death from single pathogen in cattle and other species. Rapid and explicit identification of mycobacteria is essential to hold back tuberculosis in bovines. We performed a fluorescence Peptide nucleic acid fluorescence in situ hybridization (PNA-FISH) procedure for specific detection of Mycobacterium bovis and Mycobacterium avium in bovine was optimized on cytological smears and tissue sections of bovines suspected for bovine tuberculosis.Results: PNA-FISH was performed on lung and lymph node tissues impression smears. The probes were standardized for standard bovine mycobacterial cultures at 50% formamide concentration for M.bovis and 30% formamide concentration for M.avium. All the cytological smears were positive from M.bovis probe (MTBCcy3) which was standardized at hybridization conditions of (55oC and 40% formamide) concentrations. Results revealed four out of twenty five were positive in tissue sections with a bright red fluorescence with cy3 filter (MTBC probe). No results were seen with (MAVTAMRA) probe for M.avium which was standardized at hybridization conditions of (55oC and 30% formamide) respectively. No fluorescence was seen in control tissue sections .In addition, results were juxtaposed to other commonly used detection methods like IHC and PCR by targeting esxA gene. None of the sample was found positive for M. avium.Conclusion: PNA-FISH can be used in cytological impression smears and tissue sections. It is less time consuming in diagnosis of bTB in post mortem cases than PCR.

A near-infrared fluorescence turn-on probe based on Michael addition-intramolecular cyclization for specific detection of cysteine and its applications in environment water, milk and living cells

2021 ◽  
Author(s):  
Zhigang Gao ◽  
Ling Zhang ◽  
Minchuan Yan ◽  
Haibo Liu ◽  
Shaohui Lu ◽  
...  
Keyword(s):  
Intramolecular Cyclization ◽  
Michael Addition ◽  
Near Infrared ◽  
Living Cells ◽  
Detection Methods ◽  
Biological Functions ◽  
Specific Detection ◽  
Near Infrared Fluorescence ◽  
Turn On ◽  
Fluorescence Turn On

Owing to its important biological functions in many physiological and pathological processes, it is necessary to develop efficient and appropriate detection methods for monitoring the levels of Cys in biological...

Colonization of the vegetative stage of rice plants by the false smut fungusVillosiclava virens,as revealed by a combination of species-specific detection methods

2016 ◽  
Vol 66 (1) ◽  
pp. 56-66 ◽  
Author(s):  
E. Tanaka ◽  
T. Kumagawa ◽  
N. Ito ◽  
A. Nakanishi ◽  
Y. Ohta ◽  
...  
Keyword(s):  
Detection Methods ◽  
Vegetative Stage ◽  
Specific Detection ◽  
Rice Plants ◽  
False Smut ◽  
Species Specific

scholarly journals Water Pollution Detection Based on Hypothesis Testing in Sensor Networks

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
Xu Luo ◽  
Jun Yang
Keyword(s):  
Water Pollution ◽  
Sensor Networks ◽  
Hypothesis Testing ◽  
Water Conservation ◽  
Detection Methods ◽  
Specific Detection ◽  
Detection Algorithms ◽  
Testing Theory ◽  
Pollution Detection

Water pollution detection is of great importance in water conservation. In this paper, the water pollution detection problems of the network and of the node in sensor networks are discussed. The detection problems in both cases of the distribution of the monitoring noise being normal and nonnormal are considered. The pollution detection problems are analyzed based on hypothesis testing theory firstly; then, the specific detection algorithms are given. Finally, two implementation examples are given to illustrate how the proposed detection methods are used in the water pollution detection in sensor networks and prove the effectiveness of the proposed detection methods.

scholarly journals On Some Significant Phytoplasma Diseases of Forest Trees: An Update

Forests ◽  
2021 ◽  
Vol 12 (4) ◽  
pp. 408
Author(s):  
Carmine Marcone ◽  
Deividas Valiunas ◽  
Soma Mondal ◽  
Ramachandran Sundararaj
Keyword(s):  
Fruit Trees ◽  
Detection Methods ◽  
Insect Vectors ◽  
Forest Trees ◽  
Specific Detection ◽  
Plant Host ◽  
Pcr Technology ◽  
Almost All ◽  
Host Tolerance

This paper provides an updating of information of a selected number of major phytoplasma diseases of forest trees, with a focus on the associated phytoplasma taxa. Phytoplasma diseases of forest trees have been less extensively studied than those affecting fruit trees. Research on the role of phytoplasmas as the cause of diseases of forest trees has only in the last few years been intensified, after sensitive and specific detection methods greatly based on PCR technology became available. Various phytoplasma taxa have been identified in naturally infected elm, ash, conifer, sandal, and eucalyptus trees, whereas only one phytoplasma taxon has been recorded in naturally infected alder trees. However, for almost all of the reviewed diseases, there is still sparse information about insect vectors, plant host range, strain virulence, pathogenicity, and host tolerance and resistance. Knowledge of these aspects is the basis for appropriate disease management. In particular, further research is required to clarify the role of phytoplasmas in asymptomatic trees. In addition, the etiological role of various “non-specific” phytoplasma taxa, which have been recorded in forest trees, while no data from pathological studies are available, needs to be further investigated.

Screening and construct-specific detection methods of transgenic Huafan No 1 tomato by conventional and real-time PCR

2005 ◽  
Vol 85 (13) ◽  
pp. 2159-2166 ◽  
Author(s):  
Litao Yang ◽  
Huifeng Shen ◽  
Aihu Pan ◽  
Jianxiu Chen ◽  
Cheng Huang ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Detection Methods ◽  
Specific Detection

scholarly journals Outbreak of Potato Y potyvirus and Tobacco mosaic tobamovirus in Georgia's Flue-Cured Tobacco

Plant Disease ◽  
2000 ◽  
Vol 84 (2) ◽  
pp. 201-201 ◽  
Author(s):  
H. R. Pappu ◽  
P. F. Bertrand
Keyword(s):  
Immunosorbent Assay ◽  
Growing Season ◽  
Causal Agent ◽  
Detection Methods ◽  
Enzyme Linked Immunosorbent Assay ◽  
Specific Detection ◽  
Tobacco Plants ◽  
Vein Clearing ◽  
The Past ◽  
Cucumber Mosaic Cucumovirus

Flue-cured tobacco is an economically important crop in Georgia. During the 1999 growing season, tobacco plants showing various degrees of mottling (mild to severe), mosaic, and vein clearing were observed in most counties in Georgia. In most cases, symptoms were not clearly those associated with Potato Y potyvirus (PVY) or Tobacco mosaic tobamovirus (TMV). The incidence of symptomatic plants in individual fields ranged from 0 to 90%. A survey was conducted in several tobacco-growing counties to ascertain the causal agent(s) of the disease. Symptomatic plants were collected from 11 counties: Appling, Atkinson, Bacon, Berrien, Coffee, Colquitt, Echols, Grady, Lowndes, Tattnall, and Ware. Leaf samples were tested by enzyme-linked immunosorbent assay (ELISA) for Cucumber mosaic cucumovirus (CMV), PVY, TMV, Tobacco etch potyvirus (TEV) and Tobacco vein mottling potyvirus. On the basis of ELISA, TMV and PVY were the most prevalent viruses, followed by CMV. From a total of 50 samples tested, 28 were doubly infected with TMV and PVY, 3 were positive for only TMV, and 5 were positive for only PVY. Eight samples were infected only with CMV, and one sample was doubly infected with PVY and TEV. The causal agent(s) in the remaining five samples could not be determined. Although PVY, TMV, TEV, and CMV occur to some degree every year, they have not been economically important in the past few years. It is interesting that the recent epidemic on tobacco and pepper in northern Florida was associated with CMV (1). Considering the predominance of double infections observed, diagnosis of virus-like symptoms in tobacco may require use of ELISA or other specific detection methods. Reference: (1) T. A. Kucharek et al. Plant Dis. 82:1172, 1998.

scholarly journals Rapid Detection of M. Bovis and M. Avium in Cytological Smears and Tissue Sections by PNA-FISH

2020 ◽  
Author(s):  
Rabyia Javed ◽  
Deepti Narang ◽  
Kuldip Gupta ◽  
Siddartha Deshmukh ◽  
Mudit Chandra
Keyword(s):  
Bovine Tuberculosis ◽  
Peptide Nucleic Acid ◽  
Rapid Detection ◽  
Detection Methods ◽  
Post Mortem ◽  
Specific Detection ◽  
Tissue Sections ◽  
Formamide Concentration ◽  
Pna Fish

Abstract Background: Bovine Tuberculosis is globally the paramount cause of death from single pathogen in cattle and other species. Rapid and explicit identification of mycobacteria is essential for the control of bovine tuberculosis. We performed a fluorescence Peptide nucleic acid fluorescence in situ hybridization (PNA-FISH) procedure for specific detection of Mycobacterium bovis and Mycobacterium avium in bovine was optimized on cytological smears and tissue sections of bovines suspected for bovine tuberculosis.Results: PNA-FISH was performed on lung and lymph node tissues impression smears. The probes were standardized for standard bovine mycobacterial cultures at 50% formamide concentration for M.bovis and 30% formamide concentration for M.avium. All the cytological smears were positive from M.bovis probe (MTBCcy3) which was standardized at hybridization conditions of (55oC and 40% formamide) concentrations. Results revealed 4 out of 25 were positive in tissue sections with a bright red fluorescence with cy3 filter (MTBC probe). No results were seen with (MAVTAMRA) probe for M.avium which was standardized at hybridization conditions of (55oC and 30% formamide) respectively. No fluorescence was seen in control tissue sections .In addition, results were compared to other commonly used detection methods like IHC and PCR by targeting esxA gene. None of the sample was found positive for M. avium.Conclusion: PNA-FISH can be used in cytological impression smears and tissue sections. It is less time consuming in diagnosis of bTB in post mortem cases than PCR.

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