Phospholipid Methylation and Cellular Differentiation

The behaviour of metallic powders is very important for manufacturing parts and for realize the prototypes. Manufacturing of the orthopaedic implants by selective laser sintering process can be used in medicine. The powders necessary for SLS process are biomaterials who must assure a good osteointegration of endobone implants like the Ti powder (Ti-6Al-4V) and the hydroxyapatite powder. The conditioned powders is a very important process and is necessary in SLS to obtain medical prothesis and influence the final properties of prothesis. Materials must present a gradient for porosity and one for composition (presents of a bioactive phase in titan matrix). The exterior porosity, in the contact zones with the bone tissue, favors the incipient cellular processes (the adhesion and attachment of osteoblastes, proliferation and cellular differentiation and nucleation of mineral bone). The pores must be open and with a specific surface more great. Its preferred the exterior intercommunicating porosity. The presence of phosphates of calcium in the contact zone of the implant with bone favors the mineral kernel on bone. The report Ca/P of phosphates mineral bone has an ascending evolution towards 10/6 according to hydroxyapatite.

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1116-22 Development of a transgenic mouse model for imaging of cellular differentiation into cardiomyocyte

Journal of the American College of Cardiology ◽

10.1016/s0735-1097(04)90101-5 ◽

2004 ◽

Vol 43 (5) ◽

pp. A24-A25

Author(s):

Jin Chul Paeng ◽

Dong Soo Lee ◽

Joo Hyun Kang ◽

June-Key Chung ◽

Myung Chul Lee

Keyword(s):

Mouse Model ◽

Transgenic Mouse ◽

Cellular Differentiation ◽

Transgenic Mouse Model

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SUGAR-seq enables simultaneous detection of glycans, epitopes, and the transcriptome in single cells

Science Advances ◽

10.1126/sciadv.abe3610 ◽

2021 ◽

Vol 7 (8) ◽

pp. eabe3610

Author(s):

Conor J. Kearney ◽

Stephin J. Vervoort ◽

Kelly M. Ramsbottom ◽

Izabela Todorovski ◽

Emily J. Lelliott ◽

...

Keyword(s):

Single Cell ◽

Posttranslational Modification ◽

Cellular Differentiation ◽

Single Cells ◽

Simultaneous Detection ◽

Surface Protein ◽

Rna Seq ◽

Cell Level ◽

Simultaneous Integration ◽

Unique Surface

Multimodal single-cell RNA sequencing enables the precise mapping of transcriptional and phenotypic features of cellular differentiation states but does not allow for simultaneous integration of critical posttranslational modification data. Here, we describe SUrface-protein Glycan And RNA-seq (SUGAR-seq), a method that enables detection and analysis of N-linked glycosylation, extracellular epitopes, and the transcriptome at the single-cell level. Integrated SUGAR-seq and glycoproteome analysis identified tumor-infiltrating T cells with unique surface glycan properties that report their epigenetic and functional state.

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Cellular differentiation is required for cAMP but not Ca(2+)-dependent Cl- secretion in colonic epithelial cells expressing high levels of cystic fibrosis transmembrane conductance regulator.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(18)42804-9 ◽

1992 ◽

Vol 267 (8) ◽

pp. 5575-5583

Author(s):

A.P. Morris ◽

S.A. Cunningham ◽

D.J. Benos ◽

R.A. Frizzell

Keyword(s):

Cystic Fibrosis ◽

Epithelial Cells ◽

Cellular Differentiation ◽

Transmembrane Conductance Regulator ◽

Transmembrane Conductance ◽

Colonic Epithelial Cells ◽

Cl Secretion ◽

Cystic Fibrosis Transmembrane

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Volvox: A simple algal model for embryogenesis, morphogenesis and cellular differentiation

Developmental Biology ◽

10.1016/j.ydbio.2016.07.014 ◽

2016 ◽

Vol 419 (1) ◽

pp. 99-113 ◽

Cited By ~ 28

Author(s):

Gavriel Matt ◽

James Umen

Keyword(s):

Cellular Differentiation

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Cytotoxic Escherichia coli strains encoding colibactin, cytotoxic necrotizing factor, and cytolethal distending toxin colonize laboratory common marmosets (Callithrix jacchus)

Scientific Reports ◽

10.1038/s41598-020-80000-1 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Colleen S. McCoy ◽

Anthony J. Mannion ◽

Yan Feng ◽

Carolyn M. Madden ◽

Stephen C. Artim ◽

...

Keyword(s):

Colorectal Cancer ◽

Cellular Differentiation ◽

Gastrointestinal Disease ◽

Phylogenetic Group ◽

Cytolethal Distending Toxin ◽

Whole Genome ◽

Common Marmosets ◽

E Coli ◽

Cytotoxic Necrotizing Factor ◽

Inflammatory Bowel

AbstractCyclomodulins are virulence factors that modulate cellular differentiation, apoptosis, and proliferation. These include colibactin (pks), cytotoxic necrotizing factor (cnf), and cytolethal distending toxin (cdt). Pathogenic pks+, cnf+, and cdt+ E. coli strains are associated with inflammatory bowel disease (IBD) and colorectal cancer in humans and animals. Captive marmosets are frequently afflicted with IBD-like disease, and its association with cyclomodulins is unknown. Cyclomodulin-encoding E. coli rectal isolates were characterized using PCR-based assays in healthy and clinically affected marmosets originating from three different captive sources. 139 E. coli isolates were cultured from 122 of 143 marmosets. The pks gene was detected in 56 isolates (40%), cnf in 47 isolates (34%), and cdt in 1 isolate (0.7%). The prevalences of pks+ and cnf+ E. coli isolates were significantly different between the three marmoset colonies. 98% of cyclomodulin-positive E. coli belonged to phylogenetic group B2. Representative isolates demonstrated cyclomodulin cytotoxicity, and serotyping and whole genome sequencing were consistent with pathogenic E. coli strains. However, the presence of pks+, cnf+, or cdt+ E. coli did not correlate with clinical gastrointestinal disease in marmosets. Cyclomodulin-encoding E. coli colonize laboratory common marmosets in a manner dependent on the source, potentially impacting reproducibility in marmoset models.

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