Detecting Natural Transformation of Acinetobacter Calcoaceticus, In Situ, Within Natural Epilithon Of The River Taff

1992 ◽  
pp. 253-255
Author(s):  
H. G. Williams ◽  
M. J. Day ◽  
J. C. Fry
Keyword(s):  
Natural Transformation ◽  
Acinetobacter Calcoaceticus

scholarly journals Physiological characterization of natural transformation in Acinetobacter calcoaceticus

1993 ◽  
Vol 139 (2) ◽  
pp. 295-305 ◽  
Author(s):  
R. Palmen ◽  
B. Vosman ◽  
P. Buijsman ◽  
C. K. D. Breek ◽  
K. J. Hellingwerf
Keyword(s):  
Natural Transformation ◽  
Acinetobacter Calcoaceticus ◽  
Physiological Characterization

scholarly journals Natural Transformation of Pseudomonas fluorescens and Agrobacterium tumefaciens in Soil

2001 ◽  
Vol 67 (6) ◽  
pp. 2617-2621 ◽  
Author(s):  
Sandrine Demanèche ◽  
Elisabeth Kay ◽  
François Gourbière ◽  
Pascal Simonet
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Pseudomonas Fluorescens ◽  
Natural Transformation ◽  
Physiological State ◽  
Extracellular Dna ◽  
Soil Microcosms ◽  
Do So

ABSTRACT Little information is available concerning the occurrence of natural transformation of bacteria in soil, the frequency of such events, and the actual role of this process on bacterial evolution. This is because few bacteria are known to possess the genes required to develop competence and because the tested bacteria are unable to reach this physiological state in situ. In this study we found that two soil bacteria, Agrobacterium tumefaciens and Pseudomonas fluorescens, can undergo transformation in soil microcosms without any specific physical or chemical treatment. Moreover, P. fluorescens produced transformants in both sterile and nonsterile soil microcosms but failed to do so in the various in vitro conditions we tested. A. tumefaciens could be transformed in vitro and in sterile soil samples. These results indicate that the number of transformable bacteria could be higher than previously thought and that these bacteria could find the conditions necessary for uptake of extracellular DNA in soil.

scholarly journals Reverse Transcription of 16S rRNA To Monitor Ribosome-Synthesizing Bacterial Populations in the Environment

2009 ◽  
Vol 75 (13) ◽  
pp. 4589-4598 ◽  
Author(s):  
Ting Lu ◽  
Peter G. Stroot ◽  
Daniel B. Oerther
Keyword(s):  
16S Rrna ◽  
Activated Sludge ◽  
Reverse Transcription ◽  
High Performance ◽  
Acinetobacter Calcoaceticus ◽  
Sensitive Indicator ◽  
Bacterial Populations ◽  
Pure Cultures ◽  
Physiologic State

ABSTRACT Identification and quantification of phylogenetically defined bacterial populations in the environment are often performed using molecular tools targeting 16S rRNA. Fluorescence in situ hybridization has been used to monitor the expression and processing of rRNA by targeting the 3′ tail of precursor 16S rRNA. To expand this approach, we employed reverse transcription of total RNA using primer S-D-Bact-0338-a-A-18. Length heterogeneity detected by slab gel analysis, denaturing high-performance liquid chromatography (DHPLC) was used to differentiate the 5′ tail of the precursor from mature 16S rRNA, and the relative abundance of the precursor compared to the abundance of mature 16S rRNA was shown to be a sensitive indicator of the physiologic state of Acinetobacter calcoaceticus ATCC 23055T. Our results demonstrate that this is a sensitive and reliable method with a detection limit of 10 ng of single-stranded DNA. The assay was also used to differentiate among precursor 16S rRNA levels with mixed pure cultures, as well as to examine the response of a mixed activated sludge culture exposed to fresh growth medium and the antibiotic chloramphenicol. The results of this study demonstrate that this assay is a novel reverse transcription assay that simultaneously measures the mature and precursor 16S rRNA pools for mixed bacterial populations in an engineered environment. Furthermore, collection of the reverse transcription products derived from activated sludge samples by the DHPLC approach enabled identification of the active bacterial genera. Comparison of 16S and precursor 16S rRNA clone library results indicated that the precursor 16S rRNA library is a more sensitive indicator for active bacteria in engineered environmental samples.

scholarly journals The expression of the Acinetobacter calcoaceticus recA gene increases in response to DNA damage independently of RecA and of development of competence for natural transformation

Microbiology ◽  
1996 ◽  
Vol 142 (4) ◽  
pp. 1025-1032 ◽  
Author(s):  
P. J. G. Rauch ◽  
R. Palmen ◽  
A. A. Burds ◽  
L. A. Gregg-Jolly ◽  
J. R. van der Zee ◽  
...  
Keyword(s):  
Dna Damage ◽  
Natural Transformation ◽  
Acinetobacter Calcoaceticus ◽  
Reca Gene

scholarly journals Strategy for In Situ Detection of Natural Transformation-Based Horizontal Gene Transfer Events

2007 ◽  
Vol 74 (4) ◽  
pp. 1250-1254 ◽  
Author(s):  
Aurora Rizzi ◽  
Alessandra Pontiroli ◽  
Lorenzo Brusetti ◽  
Sara Borin ◽  
Claudia Sorlini ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Natural Transformation ◽  
Plant Tissues ◽  
Acinetobacter Baylyi ◽  
Green Fluorescent ◽  
In Situ Detection ◽  
Selection Of ◽  
Selection Of Transformants

ABSTRACT A strategy is described that enables the in situ detection of natural transformation in Acinetobacter baylyi BD413 by the expression of a green fluorescent protein. Microscale detection of bacterial transformants growing on plant tissues was shown by fluorescence microscopy and indicated that cultivation-based selection of transformants on antibiotic-containing agar plates underestimates transformation frequencies.

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