Analysis of intact polar lipids by high-pressure liquid chromatography—mass spectrometry/tandem mass spectrometry with use of thermospray or atmospheric pressure ionization

1998 ◽  
pp. 290-316
Author(s):  
A. Å. Karlsson
Keyword(s):  
Mass Spectrometry ◽  
High Pressure ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
High Pressure Liquid Chromatography ◽  
Atmospheric Pressure ◽  
Tandem Mass ◽  
Liquid Chromatography Mass Spectrometry ◽  
Atmospheric Pressure Ionization ◽  
Intact Polar Lipids

scholarly journals Quantitative profiling of 1-Carbon Metabolites, Amino Acids and Precursors, and Plasmalogens in human plasma using Ultra-High-Pressure Liquid Chromatography coupled with Tandem Mass Spectrometry and robotic compound extraction

2019 ◽  
Author(s):  
Stephanie Andraos ◽  
Michael Goy ◽  
Ben Albert ◽  
Martin Kussmann ◽  
Eric B. Thorstensen ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Amino Acids ◽  
High Pressure ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Human Plasma ◽  
High Pressure Liquid Chromatography ◽  
Reversed Phase ◽  
Tandem Mass ◽  
Ultra High Pressure

AbstractAmino acids (AAs) and one-carbon (1-C) compounds are involved in a range of key metabolic pathways, and mediate numerous health and disease processes in the human body. Previous assays have quantified a limited selection of these compounds. Here, we describe an analytical method for the simultaneous quantification of 37 1-C metabolites, amino acids, precursors, and plasmalogens using reversed-phase ultra-high pressure liquid chromatography coupled with tandem mass spectrometry (UHPLC-MS/MS). Compound extraction from human plasma was tested manually before being robotically automated. The final analytical panel was validated on human plasma samples. Our automated and multiplexed method holds promise for application to large cohort studies.

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