Bovine Herpesvirus-4 (BHV-4) Infections of Cattle

1989 ◽  
pp. 96-115 ◽  
Author(s):  
E. Thiry ◽  
M. Bublot ◽  
J. Dubuisson ◽  
P.-P. Pastoret
Keyword(s):  
Bovine Herpesvirus ◽  
Bovine Herpesvirus 4 ◽  
Herpesvirus 4

scholarly journals Bovine herpesvirus 4 is tropic for bovine endometrial cells and modulates endocrine function

Reproduction ◽  
2007 ◽  
Vol 134 (1) ◽  
pp. 183-197 ◽  
Author(s):  
Gaetano Donofrio ◽  
Shan Herath ◽  
Chiara Sartori ◽  
Sandro Cavirani ◽  
Cesidio Filippo Flammini ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Stromal Cells ◽  
Bovine Herpesvirus ◽  
Endocrine Function ◽  
Uterine Disease ◽  
Endometrial Stromal Cells ◽  
Persistently Infected ◽  
Endometrial Cells ◽  
Bovine Herpesvirus 4 ◽  
Herpesvirus 4

Bovinepostpartumuterine disease, metritis, affects about 40% of animals and is widely considered to have a bacterial aetiology. Although the γ-herpesvirus bovine herpesvirus 4 (BoHV-4) has been isolated from several outbreaks of metritis or abortion, the role of viruses in endometrial pathology and the mechanisms of viral infection of uterine cells are often ignored. The objectives of the present study were to explore the interaction, tropism and outcomes of BoHV-4 challenge of endometrial stromal and epithelial cells. Endometrial stromal and epithelial cells were purified and infected with a recombinant BoHV-4 carrying an enhanced green fluorescent protein (EGFP) expression cassette to monitor the establishment of infection. BoHV-4 efficiently infected both stromal and epithelial cells, causing a strong non-apoptotic cytopathic effect, associated with robust viral replication. The crucial step for the BoHV-4 endometriotropism appeared to be after viral entry as there was enhanced transactivation of the BoHV-4 immediate early 2 gene promoter following transient transfection into the endometrial cells. Infection with BoHV-4 increased cyclooxygenase 2 protein expression and prostaglandin estradiol secretion in endometrial stromal cells, but not epithelial cells. Bovine macrophages are persistently infected with BoHV-4, and co-culture with endometrial stromal cells reactivated BoHV-4 replication in the persistently infected macrophages, suggesting a symbiotic relationship between the cells and virus. In conclusion, the present study provides evidence of cellular and molecular mechanisms, supporting the concept that BoHV-4 is a pathogen associated with uterine disease.

scholarly journals Neurological disorder in cattle associated with bovine herpesvirus 4

2011 ◽  
Vol 63 (4) ◽  
pp. 828-835 ◽  
Author(s):  
E.A. Costa ◽  
A.C. Vasconcelos ◽  
M.R.Q. Bomfim ◽  
H.B. Amorim ◽  
G.B.L. Lima ◽  
...  
Keyword(s):  
Nested Pcr ◽  
Clinical Course ◽  
Bovine Herpesvirus ◽  
Pcr Assay ◽  
Bovine Herpesvirus 1 ◽  
Herpesvirus Type ◽  
Neurological Signs ◽  
Bovine Herpesvirus 4 ◽  
Herpesvirus 1 ◽  
Herpesvirus 4

A nested PCR assay was used to diagnose bovine encephalitis through herpesviruses including bovine herpesvirus 5 (BHV-5), bovine herpesvirus 1 (BHV-1), Aujeszky's disease virus (SHV-1), and ovine herpesvirus 2 (OHV-2) in 14 fragments of central nervous system (CNS) from cattle that died with neurological signs. In addition, as some samples of bovine herpesvirus type 4 (BHV-4) have been isolated from neural tissue, it was also tested by nested PCR. The cases of encephalitis occurred in isolation at different times of the year and did not present any seasonality. The duration of the clinical course ranged between 1 to 15 days, and in 64.3% of the cases it manifested between 1 to 2 days. The most frequently observed neurological signs were ataxia, recumbency, unsteadiness and inability to stand, opisthotonus, paddling movements, nystagmus and ptyalism. In the nested assay, there was no evidence of: BHV-1, SHV-1 or OHV-2 in the DNA obtained from the CNS in any of the samples. But the presence of BHV-4 was found in all fragments of the CNS in cattle which died presenting neurological signs. Moreover, BHV-5 was found in association with BHV-4 in two of these samples.

scholarly journals Dot Immunobinding Assay for Detection of Bovine Herpesvirus 4 Antibodies in Rabbits

1999 ◽  
Vol 11 (3) ◽  
pp. 237-239 ◽  
Author(s):  
Mohamed Essmail ◽  
Dale Baker ◽  
James Collins ◽  
Sue Vandewoude ◽  
Mohamed Salman ◽  
...  
Keyword(s):  
Bovine Herpesvirus ◽  
Bovine Herpesvirus 4 ◽  
Herpesvirus 4

scholarly journals Molecular Characterization of the First Bovine Herpesvirus 4 (BoHV-4) Strains Isolated from In Vitro Bovine Embryos production in Argentina

PLoS ONE ◽  
2015 ◽  
Vol 10 (7) ◽  
pp. e0132212 ◽  
Author(s):  
Erika González Altamiranda ◽  
Julieta M. Manrique ◽  
Sandra E. Pérez ◽  
Glenda L. Ríos ◽  
Anselmo C. Odeón ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Bovine Herpesvirus ◽  
Bovine Embryos ◽  
Bovine Herpesvirus 4 ◽  
Herpesvirus 4

scholarly journals Development and Evaluation of an Indirect ELISA: Serological Survey to Detect Specific Antibodies to Bovine Herpesvirus 4

2015 ◽  
Vol 58 (5) ◽  
pp. 725-731 ◽  
Author(s):  
Ernesto Renato Krüger ◽  
Tânia Regina Penha ◽  
Fernanda Cristina Hummelgen ◽  
Jorge Bacila Agottani ◽  
Demétrio Reva ◽  
...  
Keyword(s):  
Indirect Elisa ◽  
Bovine Herpesvirus ◽  
Serological Survey ◽  
Specific Antibodies ◽  
Bovine Herpesvirus 4 ◽  
Herpesvirus 4

scholarly journals Bovine herpesvirus 4 DNA is not detected in free-ranging domestic cats from California, Colorado or Florida

2016 ◽  
Vol 19 (2) ◽  
pp. 235-239 ◽  
Author(s):  
Elliott Chiu ◽  
Ryan M Troyer ◽  
Michael R Lappin ◽  
Sue VandeWoude
Keyword(s):  
Urinary Tract ◽  
Natural Infection ◽  
Clinical Signs ◽  
Bovine Herpesvirus ◽  
Domestic Cat ◽  
Domestic Cats ◽  
High Exposure ◽  
Bovine Herpesvirus 4 ◽  
Herpesvirus 4 ◽  
Free Ranging

Objectives Several studies have reported that domestic cats can be naturally infected with bovine herpesvirus 4 (BHV4). Cats experimentally inoculated with BHV4 developed clinical signs involving the urinary tract, leading to the hypothesis that natural infection with BHV4 may be associated with feline lower urinary tract diseases. However, the question of whether BHV4 infection is common in cats remains equivocal. In this study, we sought to determine whether BHV4 is a common natural infection of domestic cats in the USA. Methods We used a sensitive nested PCR protocol specific to the BHV4 thymidine kinase gene to screen free-ranging domestic cat blood DNA samples (n = 101) collected from California, Colorado and Florida. Results Cats within this cohort were positive for seven other common pathogens of domestic cats, demonstrating the relatively high exposure of this population to endemic feline infections. In contrast, all domestic cat blood samples were negative for BHV4, while BHV4-containing tissue culture extracts were strongly positive. Conclusions and relevance BHV4 has been detected in tissues of latently infected cattle, though viral DNA is typically also detected in peripheral blood cells throughout infection. Our results suggest that persistent presence of BHV4 DNA in the blood of domestic cats is either rare or non-existent. We thus conclude that BHV4 is unlikely to be a major pathogen of cats.

Simultaneous intramammary and intranasal inoculation of lactating cows with bovine herpesvirus 4 induce subclinical mastitis

2002 ◽  
Vol 86 (1-2) ◽  
pp. 115-129 ◽  
Author(s):  
G.J Wellenberg ◽  
C.J.M Bruschke ◽  
H.J Wisselink ◽  
H.W Barkema ◽  
J.T Van Oirschot
Keyword(s):  
Bovine Herpesvirus ◽  
Subclinical Mastitis ◽  
Intranasal Inoculation ◽  
Lactating Cows ◽  
Bovine Herpesvirus 4 ◽  
Herpesvirus 4
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