Heterogeneity of genetic alterations in primary human breast tumors

1988 ◽  
pp. 25-48 ◽  
Author(s):  
Iqbal Unnisa Ali ◽  
Rosette Lidereau ◽  
Robert Callahan
Keyword(s):  
Breast Tumors ◽  
Genetic Alterations ◽  
Human Breast ◽  
Primary Human Breast

Characterization of altered intracellular drug distribution in LZ cells and primary human breast tumors

1994 ◽  
Vol 5 ◽  
pp. 7 ◽  
Author(s):  
M A Sognier ◽  
G A Altenberg ◽  
R Eberle ◽  
M Tucker ◽  
J A Belli
Keyword(s):  
Drug Distribution ◽  
Breast Tumors ◽  
Human Breast ◽  
Primary Human Breast

scholarly journals Methylation‐dependent silencing of CST6 occurs in primary human breast tumors and lymph node metastases

2007 ◽  
Vol 21 (6) ◽  
Author(s):  
Ashley G Rivenbark ◽  
Chad A Livasy ◽  
Courtney E Boyd ◽  
Daniel Keppler ◽  
William B Coleman
Keyword(s):  
Lymph Node ◽  
Lymph Node Metastases ◽  
Breast Tumors ◽  
Human Breast ◽  
Node Metastases ◽  
Primary Human Breast

scholarly journals Immunohistochemical detection of GHRH and its receptor splice variant 1 in primary human breast cancers

2004 ◽  
pp. 391-396 ◽  
Author(s):  
I Chatzistamou ◽  
AV Schally ◽  
H Kiaris ◽  
E Politi ◽  
J Varga ◽  
...  
Keyword(s):  
Splice Variant ◽  
Polyclonal Antibodies ◽  
Breast Tumors ◽  
In Vivo Studies ◽  
Breast Cancers ◽  
Human Breast ◽  
Ghrh Receptor ◽  
Primary Human Breast

OBJECTIVE: GHRH is secreted by the hypothalamus and, upon binding to specific GHRH receptors in the pituitary, stimulates growth hormone (GH) production and release from the pituitary. In addition to this neuroendocrine action, accumulated evidence implies additional roles for GHRH in carcinogenesis in non-pituitary tissues. In vitro and in vivo studies have shown that splice variant 1 (SV1) of the GHRH receptor, which is widely expressed in non-pituitary tissues and cancers, can mediate the proliferative effects of GHRH. The aim of the present study was to investigate the operation of an autocrine stimulatory loop between GHRH and SV1 in primary breast tumors. DESIGN: Fifty-three primary breast tumors were evaluated for GHRH and SV1 expression. METHODS: Expression of GHRH and SV1 was assessed by immunohistochemistry using anti-GHRH SV95 and anti-SV1 2317/5 polyclonal antibodies. RESULTS: About 40% of the specimens tested express GHRH and/or SV1 (approx. 25% each), while in 35% of these positive specimens co-expression of these antigens was detected (P<0.01). Furthermore, a correlation of GHRH, but not SV1, expression was detected in lobular compared with ductal carcinomas. CONCLUSIONS: These results constitute the first demonstration for the expression of GHRH and SV1 in primary breast cancers, and provide evidence for the operation of an autocrine stimulatory loop between GHRH and SV1 in primary cancers. Our findings indicate that GHRH analogs could have diagnostic and therapeutic applications for the management of breast cancer.

NME1 Protein Expression and Loss of Heterozygosity Mutations in Primary Human Breast Tumors

1994 ◽  
Vol 86 (15) ◽  
pp. 1167-1169 ◽  
Author(s):  
C. S. Cropp ◽  
R. Lidereau ◽  
A. Leone ◽  
D. Liscia ◽  
A. P. M. Cappa ◽  
...  
Keyword(s):  
Protein Expression ◽  
Loss Of Heterozygosity ◽  
Breast Tumors ◽  
Human Breast ◽  
Primary Human Breast

Definition of regions of the human genome affected by loss of heterozygosity in primary human breast tumors

1993 ◽  
Vol 53 (S17G) ◽  
pp. 167-172 ◽  
Author(s):  
Robert Callahan ◽  
Craig Cropp ◽  
Zong Mei Sheng ◽  
Giorgio Merlo ◽  
Patricia Steeg ◽  
...  
Keyword(s):  
Human Genome ◽  
Loss Of Heterozygosity ◽  
Breast Tumors ◽  
Human Breast ◽  
Primary Human Breast ◽  
Definition Of

scholarly journals Quantifying Breast Cancer-Driven Fiber Alignment and Collagen Deposition in Primary Human Breast Tissue

2021 ◽  
Vol 9 ◽  
Author(s):  
Rakesh Gurrala ◽  
C. Ethan Byrne ◽  
Loren M. Brown ◽  
Rafael Felix P. Tiongco ◽  
Margarite D. Matossian ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Cancer Cell ◽  
Breast Tissue ◽  
Breast Tumors ◽  
Collagen Deposition ◽  
Human Breast ◽  
Human Breast Tissue ◽  
Fiber Alignment ◽  
Primary Human Breast

Solid tumor progression is significantly influenced by interactions between cancer cells and the surrounding extracellular matrix (ECM). Specifically, the cancer cell-driven changes to ECM fiber alignment and collagen deposition impact tumor growth and metastasis. Current methods of quantifying these processes are incomplete, require simple or artificial matrixes, rely on uncommon imaging techniques, preclude the use of biological and technical replicates, require destruction of the tissue, or are prone to segmentation errors. We present a set of methodological solutions to these shortcomings that were developed to quantify these processes in cultured, ex vivo human breast tissue under the influence of breast cancer cells and allow for the study of ECM in primary breast tumors. Herein, we describe a method of quantifying fiber alignment that can analyze complex native ECM from scanning electron micrographs that does not preclude the use of replicates and a high-throughput mechanism of quantifying collagen content that is non-destructive. The use of these methods accurately recapitulated cancer cell-driven changes in fiber alignment and collagen deposition observed by visual inspection. Additionally, these methods successfully identified increased fiber alignment in primary human breast tumors when compared to human breast tissue and increased collagen deposition in lobular breast cancer when compared to ductal breast cancer. The successful quantification of fiber alignment and collagen deposition using these methods encourages their use for future studies of ECM dysregulation in human solid tumors.

Nek8, a NIMA family kinase member, is overexpressed in primary human breast tumors

Gene ◽  
2004 ◽  
Vol 328 ◽  
pp. 135-142 ◽  
Author(s):  
Alex J. Bowers ◽  
John F. Boylan
Keyword(s):  
Breast Tumors ◽  
Human Breast ◽  
Primary Human Breast

scholarly journals Amplification and over-expression of erbB-2 oncogene in primary human breast tumors

1996 ◽  
Vol 28 (1) ◽  
pp. 47-53 ◽  
Author(s):  
Jae-won Joh ◽  
Dong-Young Noh ◽  
Chang-Dae Bae ◽  
Kuk-Jin Choi ◽  
Joo-Bae Park
Keyword(s):  
Breast Tumors ◽  
Human Breast ◽  
Over Expression ◽  
Primary Human Breast
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