Effects of Ubenimex on Proliferation and Differentiation of Human Bone Marrow Cells and Leukemic Cell Lines, and the Possible Mechanisms of Its Action

1990 ◽  
pp. 81-87 ◽  
Author(s):  
K. Shibuya ◽  
S. Chiba ◽  
M. Hino ◽  
T. Kitamura ◽  
K. Miyazono ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Cell Lines ◽  
Bone Marrow Cells ◽  
Leukemic Cell ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Leukemic Cell Lines ◽  
Proliferation And Differentiation ◽  
Marrow Cells

scholarly journals Mitotic Indices of Human Bone Marrow Cells. I. Number and Cytologic Distribution of Mitoses

Blood ◽  
1962 ◽  
Vol 19 (6) ◽  
pp. 743-750 ◽  
Author(s):  
SVEN-ÅGE KILLMANN ◽  
EUGENE P. CRONKITE ◽  
T. M. FLIEDNER ◽  
VICTOR P. BOND
Keyword(s):  
Bone Marrow ◽  
Diurnal Variation ◽  
Mitotic Index ◽  
Cell Lines ◽  
Bone Marrow Cells ◽  
Cell Types ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Maturation Stages ◽  
Marrow Cells

Abstract 1. The diurnal variation of mitotic index of human bone marrow has been studied. A diurnal variation has not been demonstrated but remains subject for further study. 2. Data on the distribution of hone marrow mitoses on cell lines and maturation stages are presented. From this and the gross mitotic index of the bone marrow, the mitotic indices of various cell types are computed.

Utility of Quantum Dots for Labeling and Tracking Leukemic Cell Lines, Human Bone Marrow and CD 34+ Umbilical Cord Blood.

Blood ◽  
2005 ◽  
Vol 106 (11) ◽  
pp. 1729-1729
Author(s):  
Edward B. Garon ◽  
Gay M. Crooks ◽  
Oxana Tcherniantchouk ◽  
Quang T. Luong ◽  
Thanassis Papaioannou ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Quantum Dots ◽  
Cell Lines ◽  
Leukemic Cell ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Cell Divisions ◽  
Leukemic Cell Lines ◽  
Cd34 Cells ◽  
Normal Human

Abstract Quantum dots (QDs) are nanometer scale fluorescent semiconductors that have recently garnered much interest as agents for imaging. QDs have several advantages over conventional fluorescent imaging probes, including high quantum yield, broad absorption spectra and minimal photobleaching. QDs have been used little in hematology. We studied hematologic cells to determine whether they could be labeled with QDs, whether QDs could be seen after multiple cell divisions, whether QDs remained visible as cells differentiate and whether QDs could target specific antigens on the surface of cells. Using QDs attached to an endocytosed protein, we could intracellularly label all hematologic cells tested, including multiple leukemic cell lines (HL-60 and KG-1), normal human and murine bone marrow, leukemic human bone marrow (AML and CML) and normal human CD34+ cells. The QDs are easily detected by FACS and microscopy. By counting cell divisions using the cell membrane dye PKH26, we show that QDs can be seen in leukemic cell lines and primary cells through more than four cell divisions. Some cells from leukemic cell lines retain QDs for up to two weeks. We are also able to see QDs in cells through differentiation. We labeled HL-60 cells and cultured them with either 1,25 dihydroxy vitamin D or DMSO. QDs were seen in monocytic-like and neutrophil-like progeny of the labeled HL-60 cells. After labeling CD34+ cells and culturing them in appropriate cytokines, QDs were observed in the resultant monocytes. We were also able to target QDs specifically to cells expressing certain cell surface antigens. QDs complexed to streptavidin and a biotinylated anti-CD33 antibody homed to myelogenous leukemic cell lines but not lymphomatous cell lines. In summary, we show for the first time that QDs effectively label and track hematolgic cells, adding a new option when addressing cell fate questions in hematology.

scholarly journals Interleukin-3 is significantly more effective than other colony- stimulating factors in long-term maintenance of human bone marrow- derived colony-forming cells in vitro

Blood ◽  
1989 ◽  
Vol 73 (7) ◽  
pp. 1836-1841 ◽  
Author(s):  
M Kobayashi ◽  
BH Van Leeuwen ◽  
S Elsbury ◽  
ME Martinson ◽  
IG Young ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Cultured Cells ◽  
Bone Marrow Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Interleukin 3 ◽  
Gm Csf ◽  
Factor G ◽  
Marrow Cells

Abstract Human bone marrow cells cultured for 21 days in the presence of recombinant human interleukin-3 (IL-3) produced up to 28 times more colony-forming cells (CFC) than could be obtained from cultures stimulated with granulocyte colony stimulating factor (G-CSF) or granulocyte-macrophage CSF (GM-CSF). IL-3-cultured cells retained a multipotent response to IL-3 in colony assays but were restricted to formation of granulocyte colonies in G-CSF and granulocyte or macrophage colonies in GM-CSF. Culture of bone marrow cells in IL-3 also led to accumulation of large numbers of eosinophils and basophils. These data contrast with the effects of G-CSF, GM-CSF, and IL-3 in seven-day cultures. Here both GM-CSF and IL-3 amplified total CFC that had similar multipotential colony-forming capability in either factor. G-CSF, on the other hand, depleted IL-3-responsive colony-forming cells dramatically, apparently by causing these cells to mature into granulocytes. The data suggest that a large proportion of IL-3- responsive cells in human bone marrow express receptors for G-CSF and can respond to this factor, the majority becoming neutrophils. Furthermore, the CFC maintained for 21 days in IL-3 may be a functionally distinct population from that produced after seven days culture of bone marrow cells in either IL-3 or GM-CSF.

Enhancement of Adenovirus-Mediated Gene Transfer to Human Bone Marrow Cells

1998 ◽  
Vol 29 (5-6) ◽  
pp. 439-451 ◽  
Author(s):  
Tsutomu Watanabe ◽  
Linda Kelsey ◽  
Ana Ageitos ◽  
Charles Kuszynski ◽  
Kazuhiko Ino ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Gene Transfer ◽  
Bone Marrow Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Marrow Cells

scholarly journals The Effect of Influenza Vaccines on Maturation of Dendritic Cells Generated from Bone Marrow

2021 ◽  
Vol 5 (1) ◽  
Author(s):  
Kostinova AM ◽  
◽  
Yukhacheva DV ◽  
Akhmatova EA ◽  
Akhmatova NK ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Dendritic Cells ◽  
Bone Marrow Cells ◽  
Human Bone ◽  
Influenza Vaccines ◽  
Human Bone Marrow ◽  
Vitro Model ◽  
Cytokine Stimulation ◽  
Marrow Cells

Background: Possibility to control immune system by regulating the activity of Dendritic Cells (DC) with the help of vaccines or other immunobiological drugs opens great prospects for infectious, oncological and autoimmune control. The aim of this study was to evaluate in vitro the effect of adjuvant subunit and non-adjuvant split influenza vaccines on maturation of DCs from human bone marrow. Methods: From bone marrow cells of healthy volunteers, DCs were obtained using rGM-CSF and IL-4. On the 8th day of cultivation, 10μl of vaccines against influenza were introduced into the culture of Immature DCs (i-DCs): a non-adjuvant split vaccine (Vaxigripp, Sanofi Pasteur) and an immunoadjuvant subunit vaccine (Grippol plus, Petrovax), as well as immunomodulator Polyoxidonium. Results: Insertion of influenza vaccines into i-DC culture induced the acquisition by DCs typical morphological signs of maturation. DCs became large with eccentrically located of irregular shape nucleus, densified cytoplasm, numerous processes. By immunophenotypic examination decrease in monocyte/macrophage pool, cells with expression of CD34 immaturity marker, increase in expressing CD11c/CD86 costimulatory molecules and CD83 terminal differentiation molecules were observed. Although Polyoxidonium caused a decrease in number of CD11c/CD14 cells (18, 5%), but compared to vaccines, its activity was lower (p<0, 05). Grippol plus more actively induced differentiation of TLR2 and TLR8 expressing cells, whereas Vaxigripp-expression of TLR4 and TLR8 on DCs. Conclusion: The possibility of using in vitro model of DCs obtained from human bone marrow cells by cytokine stimulation for examination of the ability of influenza vaccines to induce DC maturation processes has been demonstrated.

scholarly journals A Model for Sequestration of the Transmission Stages of Plasmodium falciparum: Adhesion of Gametocyte-Infected Erythrocytes to Human Bone Marrow Cells

2000 ◽  
Vol 68 (6) ◽  
pp. 3455-3462 ◽  
Author(s):  
Nicola J. Rogers ◽  
Belinda S. Hall ◽  
Jacktone Obiero ◽  
Geoffrey A. T. Targett ◽  
Colin J. Sutherland
Keyword(s):  
Bone Marrow ◽  
Plasmodium Falciparum ◽  
Bone Marrow Cells ◽  
Marrow Cell ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Mature Stage ◽  
High Avidity ◽  
Necrosis Factor Alpha ◽  
Marrow Cells

ABSTRACT With the aim of developing an appropriate in vitro model of the sequestration of developing Plasmodium falciparumsexual-stage parasites, we have investigated the cytoadherence of gametocytes to human bone marrow cells of stromal and endothelial origin. Developing stage III and IV gametocytes, but not mature stage V gametocytes, adhere to bone marrow cells in significantly higher densities than do asexual-stage parasites, although these adhesion densities are severalfold lower than those encountered in classical CD36-dependent assays of P. falciparum cytoadherence. This implies that developing gametocytes undergo a transition from high-avidity, CD36-mediated adhesion during stages I and II to a lower-avidity adhesion during stages III and IV. We show that this adhesion is CD36 independent, fixation sensitive, stimulated by tumor necrosis factor alpha, and dependent on divalent cations and serum components. These data suggest that gametocytes and asexual parasites utilize distinct sets of receptors for adhesion during development in their respective sequestered niches. To identify receptors for gametocyte-specific adhesion of infected erythrocytes to bone marrow cells, we tested a large panel of antibodies for the ability to inhibit cytoadherence. Our results implicate ICAM-1, CD49c, CD166, and CD164 as candidate bone marrow cell receptors for gametocyte adhesion.

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