Infectious Diseases, Vibrational Spectroscopic Approaches to Rapid Diagnostics

PO 8460 PANDORA-ID NET (PAN-AFRICAN NETWORK FOR RAPID RESEARCH, RESPONSE, RELIEF AND PREPAREDNESS FOR INFECTIOUS DISEASES EPIDEMICS)

BMJ Global Health ◽

10.1136/bmjgh-2019-edc.105 ◽

2019 ◽

Vol 4 (Suppl 3) ◽

pp. A40.2-A40

Author(s):

Francine Ntoumi ◽

Francine Zumla ◽

Giuseppe Ippolito ◽

Francesco Vairo

Keyword(s):

Public Health ◽

Infectious Diseases ◽

Animal Health ◽

Disease Outbreaks ◽

Control Measures ◽

Rapid Diagnostics ◽

Public Health Response ◽

Effective Response ◽

Zoonotic Infections ◽

Pan African

BackgroundNew and re-emerging infectious disease outbreaks continue to cause much human suffering and loss of life worldwide. Since Africa has experienced repeated outbreaks of zoonotic infections, an important need exists to improve local and regional capacities to identify and respond to zoonotic outbreaks. PANDORA ID-NET is an EDCTP-supported ‘ONE Human and Animal HEALTH’ multidisciplinary consortium of 24 partner institutions (15 African and 9 European) in 9 African and 4 European countries.MethodsOur overall aim is to strengthen regional and pan-African capacities and systems for enabling a rapid and effective response to infectious diseases with epidemic potential, arising from within Africa or imported from overseas. We aim to build laboratory and public health capabilites for rapid detection and surveillance of pathogens from human and animal sources. This will include obtaining accelerated evidence for optimal clinical management of patients, infection control measures, and public health response during outbreaks. Capacities will be built: a) for performing multisite clinical trials (evaluating rapid diagnostics, biomarkers, a range of treatments, vaccines and operational research studies) and, b) for timely collection, analysis and communication of information.ConclusionOur activities will be aligned to EDCTP regional Networks of Excellence, Africa CDC and other relevant global and regional initiatives, thus maximizing complementarity and achieving a multiplier effect, facilitating rapid policy implementation of outputs.

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Rapid Electronic Diagnostics of Ebola Virus with Synthetic Nanobody-Conjugated Gold Nanoparticles

10.1101/2021.05.09.443341 ◽

2021 ◽

Author(s):

Xiahui Chen ◽

Shoukai Kang ◽

MD Ashif Ikbal ◽

Zhi Zhao ◽

Jiawei Zuo ◽

...

Keyword(s):

Gold Nanoparticles ◽

Infectious Diseases ◽

Ebola Virus ◽

Semiconductor Device ◽

Point Of Care ◽

Colorimetric Detection ◽

Emerging Infectious Diseases ◽

Rapid Diagnostics ◽

Point Of Care Testing ◽

Training Requirement

The success of controlling emerging infectious diseases relies on the fast development of robust, quantitative assays for point-of-care testing. Here a generalizable strategy is demonstrated for developing inexpensive, simple-to-use, and rapid diagnostics within a few weeks upon the identification of a new viral antigen. Using Ebola virus secreted glycoprotein (sGP) as a target, we design a new assay featuring nanobody-conjugated nanoparticles for rapid, electronic detection (Nano2RED). Nanobodies with the high affinity and specificity were generated by phage display screening of a high-quality combinatorial library (> 109) and site-specifically conjugated to gold nanoparticles (AuNPs) for in-solution colorimetric detection. Our assay can robustly detect the sGP protein from 10 pM to 100 nM in diluted serum and distinguish it from a membrane-anchored isoform, GP1,2, allowing the diagnosis of the viral infection stage. Additionally, a rapid assay protocol was established to decrease the assay time to a few minutes without compromising the accuracy. Lastly, this assay has been integrated with a portable semiconductor device with a digital readout and minimal training requirement for end users. Our method can be widely applied to the point-of-care testing of other infectious diseases.

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Systematic Evolution of Ligands by Exponential Enrichment Technologies and Aptamer-Based Applications: Recent Progress and Challenges in Precision Medicine of Infectious Diseases

Frontiers in Bioengineering and Biotechnology ◽

10.3389/fbioe.2021.704077 ◽

2021 ◽

Vol 9 ◽

Author(s):

Yixin Xu ◽

Xin Jiang ◽

Yanhong Zhou ◽

Ming Ma ◽

Minjin Wang ◽

...

Keyword(s):

Infectious Diseases ◽

Targeted Drug Delivery ◽

Early Recognition ◽

Global Public Health ◽

Rapid Diagnostics ◽

Comprehensive Overview ◽

Precision Therapy ◽

Exciting Potential ◽

Systematic Evolution ◽

Exponential Enrichment

Infectious diseases are considered as a pressing challenge to global public health. Accurate and rapid diagnostics tools for early recognition of the pathogen, as well as individualized precision therapy are essential for controlling the spread of infectious diseases. Aptamers, which were screened by systematic evolution of ligands by exponential enrichment (SELEX), can bind to targets with high affinity and specificity so that have exciting potential in both diagnosis and treatment of infectious diseases. In this review, we provide a comprehensive overview of the latest development of SELEX technology and focus on the applications of aptamer-based technologies in infectious diseases, such as targeted drug-delivery, treatments and biosensors for diagnosing. The challenges and the future development in this field of clinical application will also be discussed.

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