Ethnicity and Ovarian Response: The Role of FSH Receptor Genotype

Abstract Because the ovarian response to FSH stimulation in assisted reproduction is variable, ranging from hyporesponse to hyperresponse, with the possible complication of ovarian hyperstimulation, it would be of great benefit to predict the response of the patients to FSH. To date, no clear-cut predictors of ovarian responsiveness to FSH have been identified. In this study, we investigated the role of two distinct FSH receptor (FSHR) variants, Thr307/Asn680 and Ala307/Ser680, in the response to FSH in women undergoing controlled ovarian stimulation. The FSHR polymorphism at position 680 was analyzed by restriction-fragment-length polymorphism in 161 ovulatory women below the age of 40 yr. With reference to the couple, infertility has been diagnosed as being attributable to male causes (76%), tubal factor (11%), or both (13%). The distribution was 29% for the Asn/Asn, 45% for the Asn/Ser, and 26% for the Ser/Ser FSHR variant. Peak estradiol levels, number of preovulatory follicles, and number of retrieved oocytes were similar in the 3 groups. However, basal FSH levels were significantly different among the 3 groups (6.4 ± 0.4 IU/L, 7.9 ± 0.3 IU/L, and 8.3 ± 0.6 IU/L for the Asn/Asn, Asn/Ser, and Ser/Ser groups, respectively, P < 0.01). The number of FSH ampoules required for successful stimulation was significantly different among the 3 groups (31.8 ± 2.4, 40.7 ± 2.3, and 46.8 ± 5.0 for the Asn/Asn, Asn/Ser, and Ser/Ser groups, respectively, P < 0.05). According to multiple linear regression analysis, the number of ampoules needed could be predicted from a linear combination of both the type of polymorphism and basal FSH levels (P < 0.001). These clinical findings demonstrate that the ovarian response to FSH stimulation depends on the FSHR genotype.

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The Role of FSHR SNPs and AMH in Follicular Fluid and Serum in Ovarian Response during COS: A Pilot Study

International Journal of Reproductive Medicine ◽

10.1155/2021/8685158 ◽

2021 ◽

Vol 2021 ◽

pp. 1-10

Author(s):

Elli Anagnostou ◽

Despina Mavrogianni ◽

Ilectra-Niki Prifti ◽

Evangelia Dimitroulia ◽

Athanasios Protopapas ◽

...

Keyword(s):

Follicular Fluid ◽

Oocyte Retrieval ◽

Ovarian Response ◽

Prognostic Indicator ◽

Fsh Receptor ◽

Oocyte Collection ◽

Significant Difference ◽

Fluid Levels

Background. Several studies have investigated on the polymorphism Ser680Asn of FSHR and its use as a predictive indicator of response to an IVF/ICSI protocol. Furthermore, measurement of AMH in serum and follicular fluid is a useful prognostic indicator for the outcome of an assisted reproduction attempt. The purpose of this study is to examine the FSH receptor Ser680Asn polymorphism in combination with AMH levels in both serum and follicular fluid, on the day of oocyte collection. Materials and Methods. A total of 32 women who underwent IVF/ICSI were included. Women were grouped into 2 groups: those who received rFSH (n=11) and those who received hMG (n=21). Serum AMH was measured on day 3 of the cycle, and AMH in the follicular fluid on the day of oocyte retrieval; the same day peripheral blood was collected for the genotyping of Ser680Asn. Results. No statistical significant difference was found between serum AMH and follicular fluid AMH regarding the FSH receptor genotype for the Ser680Asn polymorphism. Regarding the sAMH/ffAMH ratio in the 3 genotypes, the value was lower in Asn/Asn women than Ser/Ser and Ser/Asn, but no statistical difference was obtained. Women who carry the Ser allele have a higher number of follicles, retrieved oocytes, and mature oocytes than women who do not contain the Ser allele. Women withAMH<2.22 ng/mlpresented lower AMH follicular fluid levels and lower serum AMH/follicular fluid AMH ratio in a statistically significant manner. Concerning the genotype for the polymorphism Ser680Asn of FSHR in relation to AMH levels, no statistically significant differences were found. Conclusions. The identification of polymorphisms, such as Ser680Asn of FSHR, along with the determination of endocrine markers in the follicular fluid, such as AMH, could lead at some point, to the personalized therapy setting per woman.

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The role of maternal age in the assisted reproductive technologies

Reproductive Medicine Review ◽

10.1017/s0962279999000149 ◽

1999 ◽

Vol 7 (1) ◽

pp. 41-60 ◽

Cited By ~ 9

Author(s):

Mark A Damario ◽

Owen K Davis ◽

Zev Rosenwaks

Keyword(s):

Older Women ◽

Assisted Reproductive Technologies ◽

Clinical Importance ◽

Ovarian Response ◽

Reproductive Technologies ◽

Abortion Rate ◽

Western World ◽

Delayed Childbearing ◽

Art Outcome

Age is perhaps the most important single variable influencing outcome in the assisted reproductive technologies (ART). The effect of advancing age on clinical ART outcome is manifested not only in the pattern of ovarian response to stimulation regimens, but also in reduced implantation efficiency and an increased spontaneous abortion rate. The clinical importance of these factors is compounded by the fact that increasing numbers of older women are presenting for ART treatment. Delayed childbearing is becoming increasingly common in the western world. The availability of methods of birth control, educational and career priorities for women, and the increased rates of divorce and remarriage are some of the factors contributing to this phenomenon.

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Influence of FSH Receptor Genotype on Ovarian Response to FSH in Brazilian Women Undergoing Assisted Reproduction

10.1210/endo-meetings.2011.part2.p30.p2-205 ◽

2011 ◽

pp. P2-205-P2-205

Author(s):

Cintia Tusset ◽

Mariangela Badalotti ◽

Ericka Trarbach ◽

Mirian Yumie Nishi ◽

Berenice Bilharinho Mendonca ◽

...

Keyword(s):

Assisted Reproduction ◽

Ovarian Response ◽

Fsh Receptor

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The Exploration of Poor Ovarian Response–Related Risk Factors: A Potential Role of Growth Differentiation Factor 8 in Predicting Ovarian Response in IVF-ET Patient

Frontiers in Endocrinology ◽

10.3389/fendo.2021.708089 ◽

2021 ◽

Vol 12 ◽

Author(s):

Long Bai ◽

Huihui Pan ◽

Yinjun Zhao ◽

Qingqing Chen ◽

Yu Xiang ◽

...

Keyword(s):

Risk Factors ◽

Growth Factor ◽

Potential Role ◽

Transforming Growth Factor ◽

Ovarian Response ◽

Poor Ovarian Response ◽

Aberrant Expression ◽

Differentiation Factor ◽

Ivf Et

Controlled ovarian hyperstimulation (COH) is the most common therapeutic protocol to obtain a considerable number of oocytes in IVF-ET cycles. To date, the risk factors affecting COH outcomes remain elusive. Growth differentiation factor 8 (GDF-8), a member of transforming growth factor β (TGF-β) superfamily, has been long discerned as a crucial growth factor in folliculogenesis, and the aberrant expression of GDF-8 is closely correlated with the reproductive diseases. However, less is known about the level of GDF-8 in IVF-ET patients with different ovarian response. In the present study, the potential risk factors correlated with ovarian response were explored using logistic regression analysis methods. Meanwhile, the expression changes of GDF-8 and its responsible cellular receptors in various ovarian response patients were determined. Our results showed that several factors were intensely related to poor ovarian response (POR), including aging, obesity, endometriosis, surgery history, and IVF treatment, while irregular menstrual cycles and PCOS contribute to hyperovarian response (HOR). Furthermore, POR patients exhibited a decrease in numbers of MII oocytes and available embryos, thereby manifesting a lower clinical pregnancy rate. The levels of GDF-8, ALK5, and ACVR2B in POR patients were higher compared with those in control groups, whereas the expression level of ACVR2A decreased in poor ovarian response patients. In addition, clinical correlation analysis results showed that the concentration of GDF-8 was negatively correlated with LH and estradiol concentration and antral follicle count. Collectively, our observations provide a novel insight of ovarian response–associated risk factors, highlighting the potential role of GDF-8 levels in ovarian response during COH process.

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