scholarly journals The Role of FSHR SNPs and AMH in Follicular Fluid and Serum in Ovarian Response during COS: A Pilot Study

2021 ◽  
Vol 2021 ◽  
pp. 1-10
Author(s):  
Elli Anagnostou ◽  
Despina Mavrogianni ◽  
Ilectra-Niki Prifti ◽  
Evangelia Dimitroulia ◽  
Athanasios Protopapas ◽  
...  
Keyword(s):  
Follicular Fluid ◽  
Oocyte Retrieval ◽  
Ovarian Response ◽  
Prognostic Indicator ◽  
Fsh Receptor ◽  
Oocyte Collection ◽  
Significant Difference ◽  
Fluid Levels

Background. Several studies have investigated on the polymorphism Ser680Asn of FSHR and its use as a predictive indicator of response to an IVF/ICSI protocol. Furthermore, measurement of AMH in serum and follicular fluid is a useful prognostic indicator for the outcome of an assisted reproduction attempt. The purpose of this study is to examine the FSH receptor Ser680Asn polymorphism in combination with AMH levels in both serum and follicular fluid, on the day of oocyte collection. Materials and Methods. A total of 32 women who underwent IVF/ICSI were included. Women were grouped into 2 groups: those who received rFSH (n=11) and those who received hMG (n=21). Serum AMH was measured on day 3 of the cycle, and AMH in the follicular fluid on the day of oocyte retrieval; the same day peripheral blood was collected for the genotyping of Ser680Asn. Results. No statistical significant difference was found between serum AMH and follicular fluid AMH regarding the FSH receptor genotype for the Ser680Asn polymorphism. Regarding the sAMH/ffAMH ratio in the 3 genotypes, the value was lower in Asn/Asn women than Ser/Ser and Ser/Asn, but no statistical difference was obtained. Women who carry the Ser allele have a higher number of follicles, retrieved oocytes, and mature oocytes than women who do not contain the Ser allele. Women withAMH<2.22 ng/mlpresented lower AMH follicular fluid levels and lower serum AMH/follicular fluid AMH ratio in a statistically significant manner. Concerning the genotype for the polymorphism Ser680Asn of FSHR in relation to AMH levels, no statistically significant differences were found. Conclusions. The identification of polymorphisms, such as Ser680Asn of FSHR, along with the determination of endocrine markers in the follicular fluid, such as AMH, could lead at some point, to the personalized therapy setting per woman.

132 Influence of oocyte retrieval methods and maturation media on invitro development of polar body extrusion in pig oocytes

2021 ◽  
Vol 33 (2) ◽  
pp. 174
Author(s):  
K. M. Honneysett ◽  
M. L. Mphaphathi ◽  
A. M. Maqhashu ◽  
E. C. Webb
Keyword(s):  
Follicular Fluid ◽  
Cell Layer ◽  
Polar Body ◽  
Cumulus Cell ◽  
Oocyte Retrieval ◽  
Cumulus Cells ◽  
Reproductive Technology ◽  
Maturation Stage ◽  
Significant Difference ◽  
Polar Body Extrusion

Oocyte recovery is a reproductive technology that can be done by using two techniques, aspiration and slicing. Invitro maturation (IVM) is an additional reproductive technology used to advance an oocyte to a maturation stage; thereafter, it may be used during IVF. The objectives of the present study were (1) to compare two different oocyte retrieval methods (aspiration and slicing) from pig ovaries on oocyte quality and quantity, and (2) to compare three different IVM media [NCSU 37, TCM-199, and modified porcine follicular fluid (mpFF=porcine follicular fluid+FSH+LH] on oocytes’ polar body extrusion. During aspiration, an 18G needle was attached to a 10-mL syringe and all visible follicles were aspirated. During slicing, a surgical blade was used to slice the ovaries held in mDPBS. Follicular fluid collected from both methods was assessed for the presence of oocytes with the aid of a microscope. The collected oocytes were then categorized as Grade A, B, or C: Grade A=oocytes with compacted, multilayered cumulus cells and a homogeneous ooplasm; Grade B=oocytes with a compact cumulus cell layer with homogeneous ooplasm; Grade C=oocytes with a less compact cumulus cell layer with irregular ooplasm containing dark granules. The IVM media were placed in a four-well multidish; thereafter Grades A and B oocytes were allocated per treatment groups and matured for 44h. The treatment means were compared using the Fisher’s protected t-test least significant difference. The results showed significant differences between the grades of oocytes (P&lt;0.05) with Grade A and B oocytes accounting for 50.8% of total oocytes (193.8) for aspiration and 58.7% of total oocytes (488.6) for slicing. The oocytes polar body extrusion was recorded as 25.3, 84.2, and 73.8% for NCSU 37 (P&lt;0.05) and TCM-199 and mpFF respectively (P&gt;0.05). In conclusion, the slicing method proved to be better than aspiration with regards to the retrieval of Grades A and B oocytes as well as the total number of oocytes retrieved. The TCM-199 and mpFF media had a higher percentage of oocytes with polar body extrusion than NCSU 37.

scholarly journals miR-15a-5p levels correlate with poor ovarian response in human follicular fluid

Reproduction ◽  
2017 ◽  
Vol 154 (4) ◽  
pp. 483-496 ◽  
Author(s):  
Kaiyue Zhang ◽  
Wanxia Zhong ◽  
Wei-Ping Li ◽  
Zi-Jiang Chen ◽  
Cong Zhang
Keyword(s):  
Follicular Fluid ◽  
Quantitative Polymerase Chain Reaction ◽  
Young Patients ◽  
Oocyte Retrieval ◽  
Ovarian Response ◽  
Poor Ovarian Response ◽  
Human Follicular Fluid ◽  
Transfer Procedures ◽  
Response Group

Poor ovarian response is a significant problem encountered during in vitro fertilization and embryo transfer procedures. Many infertile women may suffer from poor ovarian response and its incidence tends to be increasing in young patients nowadays. It is a major cause of maternal infertility because it is associated with low pregnancy and live birth rates. However, the cause of poor ovarian response is not clear. In this study, we extracted microRNAs from human follicular fluid and performed miRNA sequencing to investigate a potential posttranscriptional mechanism underlying poor ovarian response. The results showed that many miRNAs were obviously different between the poor ovarian response and non-poor ovarian response groups. We then performed quantitative polymerase chain reaction, Western blot analysis and used an in vitro culture system to verify the sequencing results and to study the mechanism. Notably, we found that miRNA-15a-5p was significantly elevated in the young poor ovarian response group. Furthermore, we demonstrated that high levels of miR-15a-5p in the young poor ovarian response group repressed granulosa cell proliferation by regulating the PI3K-AKT-mTOR signaling pathway and promoted apoptosis through BCL2 and BAD. This could explain the reduced oocyte retrieval number seen in poor ovarian response patients.

scholarly journals Ovarian Response to Follicle-Stimulating Hormone (FSH) Stimulation Depends on the FSH Receptor Genotype*

2000 ◽  
Vol 85 (9) ◽  
pp. 3365-3369 ◽  
Author(s):  
Maritza Perez Mayorga ◽  
Jörg Gromoll ◽  
Hermann M. Behre ◽  
Claudia Gassner ◽  
Eberhard Nieschlag ◽  
...  
Keyword(s):  
Linear Regression Analysis ◽  
Ovarian Response ◽  
Multiple Linear Regression Analysis ◽  
Clinical Findings ◽  
Fsh Receptor ◽  
Clear Cut ◽  
Preovulatory Follicles ◽  
Tubal Factor ◽  
Estradiol Levels

Abstract Because the ovarian response to FSH stimulation in assisted reproduction is variable, ranging from hyporesponse to hyperresponse, with the possible complication of ovarian hyperstimulation, it would be of great benefit to predict the response of the patients to FSH. To date, no clear-cut predictors of ovarian responsiveness to FSH have been identified. In this study, we investigated the role of two distinct FSH receptor (FSHR) variants, Thr307/Asn680 and Ala307/Ser680, in the response to FSH in women undergoing controlled ovarian stimulation. The FSHR polymorphism at position 680 was analyzed by restriction-fragment-length polymorphism in 161 ovulatory women below the age of 40 yr. With reference to the couple, infertility has been diagnosed as being attributable to male causes (76%), tubal factor (11%), or both (13%). The distribution was 29% for the Asn/Asn, 45% for the Asn/Ser, and 26% for the Ser/Ser FSHR variant. Peak estradiol levels, number of preovulatory follicles, and number of retrieved oocytes were similar in the 3 groups. However, basal FSH levels were significantly different among the 3 groups (6.4 ± 0.4 IU/L, 7.9 ± 0.3 IU/L, and 8.3 ± 0.6 IU/L for the Asn/Asn, Asn/Ser, and Ser/Ser groups, respectively, P &lt; 0.01). The number of FSH ampoules required for successful stimulation was significantly different among the 3 groups (31.8 ± 2.4, 40.7 ± 2.3, and 46.8 ± 5.0 for the Asn/Asn, Asn/Ser, and Ser/Ser groups, respectively, P &lt; 0.05). According to multiple linear regression analysis, the number of ampoules needed could be predicted from a linear combination of both the type of polymorphism and basal FSH levels (P &lt; 0.001). These clinical findings demonstrate that the ovarian response to FSH stimulation depends on the FSHR genotype.

scholarly journals Mandibular Canine Index: A Reliable Sex Determinant –Original Research

2018 ◽  
Author(s):  
Nikita Rathi ◽  
shweta bhat ◽  
Rajiv Desai
Keyword(s):  
Sex Determination ◽  
Forensic Odontology ◽  
Original Research ◽  
City Population ◽  
Significant Difference ◽  
Mandibular Canine ◽  
The Right ◽  
Mumbai City

Introduction: Forensic odontology is a unique discipline dealing with evidence related to dental and oral structures. Mandibular canines are considered to be an important aid for sex determination. The aim of this study was to analyse the role of mandibular canine index in sex determination of Mumbai city population. Materials and Methodology: This was a retrospective study done on the study models of 50 patients aged between 17 to 25 years old and equally distributed among both the sexes, whose treatment has been taken place in The Department of Orthodontics, Nair Hospital Dental College, Mumbai. Mesio-distal width of mandibular canines were measured using a digital Vernier calliper and mandibular canine index was calculated using standardized equation. Independent sample t-test was used and P < 0.05 was considered statistically significant. Results: There was a significant difference (P < 0.05) observed between right and left mandibular canine width and a significant difference between the right and left mandibular canine index between males and females. Conclusion: The present study verifies the fact that mandibular canine index can be used for sex determination in the population of Mumbai city and can form basis for forensic investigations. Key-words: Canine index, Sex determination, Forensic odontology

scholarly journals Interleukin 15 and granulocytes colony stimulating factor levels in relation to ICSI outcome in subfertile PCOS women

2019 ◽  
Vol 10 (3) ◽  
pp. 1883-1890
Author(s):  
Zainab Obaid Jaddoa ◽  
Najah Shakir Al Khalil ◽  
Ahmed Abdul- Hassan Abbas
Keyword(s):  
Follicular Fluid ◽  
Ovarian Response ◽  
Control Group ◽  
Colony Stimulating Factor ◽  
Control Groups ◽  
Oocyte Number ◽  
Significant Difference ◽  
Median Level ◽  
Interleukin 15 ◽  
Stimulating Factor

Polycystic Ovary Syndrome constitutes a complex heterogeneous disorder, considered one of the leading cause of delayed conception in women. Serum and follicular fluid cytokines are subject of extensive studies to identify their role in subfertility with controversial results. This study was aimed to evaluate the relation of Granulocytes Colony Stimulating Factor (G-CSF) and Interleukin 15 (IL 15) levels to ovarian response and ICSI outcome in PCOS women undergoing ICSI. This cross-sectional study conducted at High Institute of Infertility Diagnosis and Assisted Reproductive Technique and Baghdad IVF Private Centre. Which include 80 women divided into two groups: 40 PCOS women without other causes of subfertility and 40 non PCOS women with other causes of subfertility. Ovarian hormonal stimulation conducted according to GnRH antagonist protocol, then serum & follicular fluid obtained at the day of oocyte retrieval for measurement of G-CSF & IL 15 concentration measured by ELISA method, then statistical analysis is used to correlate the concentration of the cytokine with follicle number, retrieved oocyte number, oocyte maturity, number of 2PN oocytes, number of grade1 embryones, biochemical pregnancies and clinical pregnancy rates. The results of currents study revealed that Serum estradiol (E2) on hCG day was (2238.70 ±468.95 vs. 1997.40 ±291.95 pg/ml), endometrial thickness (10.80 ±1.49 vs. 10.24 ±0.98 mm), total follicle number (15.53 ±3.86 versus 12.43 ±2.25), oocyte number (13.03 ±3.10 vs. 9.83 ±1.52), mature oocyte (MII) number (9.48 ±2.45 vs. 7.75 ±1.48) mean number of fertilized oocytes (2PN) was (6.25 ±1.41 vs. 5.35 ±1.08), being higher in PCOS women compared to the control group. On day of oocytes retrieval, both median level of serum G-CSF (54.30) (35.35) and follicular G-CSF (59.00) (38.72) levels were significantly higher in PCOS than control groups, while there was no significant difference in median level of serum IL15 (59.10) (17.75) and median level of follicular IL-15(61.40) (16.00) between PCOS and control groups.  The study concluded that in spite of higher levels of G-CSF and IL15 in serum and follicular fluid of PCOS women, a relation between G-CSF and IL15 with ovarian response could not be established in comparison to control group, however, both (G-CSF and IL15) may be a predictor of pregnancy among the same group of women both PCOS and controls.

The Correlation between Follicular Fluid Antimullerian Hormone Levels and Fertilization and Embryo Quality in ART Cycles

2012 ◽  
Vol 3 (3) ◽  
pp. 83-86
Author(s):  
Rutvij Jay Dalal ◽  
Akansha Mishra
Keyword(s):  
Granulosa Cells ◽  
Follicular Fluid ◽  
Embryo Quality ◽  
Hormone Levels ◽  
Antimullerian Hormone ◽  
Significant Difference ◽  
Antimüllerian Hormone ◽  
High Level

ABSTRACT Background Determination of oocyte and embryo quality are one of the most important goals in in vitro fertilization (IVF). Antimullerian hormone (AMH) is secreted by the ovarian granulosa cells into blood flow and follicular fluid. Follicular fluid (FF) AMH level is probably a marker of activity of granulosa cells. Objective To evaluate whether high level of FF AMH level is related to success of fertilization and better embryo quality. Materials and methods Sixty-two women, whose FF sample was obtained from a single follicle in each patient, underwent IVF with GnRH-agonist long protocol. Based on oocyte fertilization, the patients were divided into fertilized group (n = 42) and nonfertilized group (n = 20). FF AMH levels were measured in both groups and the quality of embryos was determined in fertilized group. Results Median of FF AMH level in fertilized group was higher than that in nonfertilized group (5.7 vs 2.7 ng/ml) and a statistically significant difference was observed between the two groups. There was a significant difference between FF AMH level and scores of embryos (p < 0.001). The medians levels of FF AMH were 6.7 ng/ml in good quality embryos and 3.80 ng/ml in fair quality embryos. Conclusion Our results indicate that FF AMH level has positive correlation with fertilization and embryo quality; therefore, it can be considered as a marker of IVF outcome. How to cite this article Dalal RJ, Mishra A. The Correlation between Follicular Fluid Antimullerian Hormone Levels and Fertilization and Embryo Quality in ART Cycles. Int J Infertility Fetal Med 2012;3(3):83-86.

scholarly journals Evaluation of Oxidative Stress as a Contributor of Anesthetic Agents' Effects on Embryo Quality and ICSI Outcome in Iraqi Patients

2021 ◽  
Vol 10 (2) ◽  
pp. 15-28
Author(s):  
Athraa Abd Alhussain ◽  
Raad Reshan ◽  
Hayder Mossa
Keyword(s):  
General Anesthesia ◽  
Follicular Fluid ◽  
Fluid Collection ◽  
Oocyte Retrieval ◽  
Unprotected Sex ◽  
The Other ◽  
Oocyte Maturity ◽  
Anesthetic Agents ◽  
Significant Difference ◽  
Before And After

Infertility is described as a couple's failure to conceive for at least a year, using unprotected sex. Even though all anesthetic chemicals have been revealed in the follicular fluids, general anesthesia is still used in many IVF centers for patients who want to get pregnant. This study included 60 infertile women randomly divided into two groups under general anesthesia. 30 patients were given a Ketamine dose of 0.5 mg/Kg and the other 30 patients were received Remifentanil dose of 0.5 µg/Kg for induction of anesthesia. We collected serum for Reactive Oxygen Species (ROS) levels assessment for all patients before and after starting general anesthesia. Routine ICSI procedures was performed on all participants, including clinical evaluation (history, examination, and investigation), controlled ovarian and ovulation stimulation, oocyte retrieval under general anesthesia, follicular fluid collection for postoperative anesthesia medication concentration (Remifentanil and Ketamine), oocyte stripping, oocyte maturity assessment, intra-oocyte sperm injection into mature cells (MII), fertilization and division evaluation and embryo categorization, embryo selection and transfer, luteal phase support, and beta-hCG determination. ROS concentrations were compared between the Remifentanil and Ketamine groups. There was no significant difference in embryo features between Remifentanil and Ketamine, indicating that neither one is superior to the other in this regard. When Remifentanil or Ketamine was taken, there was no significant difference in ROS levels in serum or follicular fluid.

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