Physical Mapping of DNA Sequences on Plant Chromosomes by Light Microscopy and High Resolution Scanning Electron Microscopy

1991 ◽  
pp. 277-284 ◽  
Author(s):  
Harald Lehfer ◽  
Gerhard Wanner ◽  
Reinhold G. Herrmann
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
High Resolution ◽  
Light Microscopy ◽  
Physical Mapping ◽  
Dna Sequences ◽  
Plant Chromosomes ◽  
Scanning Electron

Examination of Schistosome Cercariae and Schistosomules by Scanning Electron Microscopy

1969 ◽  
Vol 27 ◽  
pp. 50-51
Author(s):  
D. Johnson ◽  
P. Moriearty
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
High Resolution ◽  
Light Microscopy ◽  
Surface Structure ◽  
Extensive Study ◽  
Scanning Microscopy ◽  
Host Parasite ◽  
Conventional Electron Microscopy ◽  
Scanning Electron

Since several species of Schistosoma, or blood fluke, parasitize man, these trematodes have been subjected to extensive study. Light microscopy and conventional electron microscopy have yielded much information about the morphology of the various stages; however, scanning electron microscopy has been little utilized for this purpose. As the figures demonstrate, scanning microscopy is particularly helpful in studying at high resolution characteristics of surface structure, which are important in determining host-parasite relationships.

Efficient preparation of plant chromosomes for high-resolution scanning electron microscopy

1994 ◽  
Vol 2 (5) ◽  
pp. 411-415 ◽  
Author(s):  
R. Martin ◽  
W. Busch ◽  
R. G. Herrmann ◽  
G. Wanner
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
High Resolution ◽  
Plant Chromosomes ◽  
Scanning Electron

scholarly journals High-resolution imaging by scanning electron microscopy of semithin sections in correlation with light microscopy

Microscopy ◽  
2015 ◽  
Vol 64 (6) ◽  
pp. 387-394 ◽  
Author(s):  
Daisuke Koga ◽  
Satoshi Kusumi ◽  
Ryusuke Shodo ◽  
Yukari Dan ◽  
Tatsuo Ushiki
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
High Resolution ◽  
Light Microscopy ◽  
High Resolution Imaging ◽  
Semithin Sections ◽  
Resolution Imaging ◽  
Scanning Electron

Characterization of a peg-like terminal NOR structure with light microscopy and high-resolution scanning electron microscopy

Chromosoma ◽  
2005 ◽  
Vol 115 (1) ◽  
pp. 50-59 ◽  
Author(s):  
Elizabeth Schroeder-Reiter ◽  
Andreas Houben ◽  
Jürke Grau ◽  
Gerhard Wanner
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
High Resolution ◽  
Light Microscopy ◽  
Scanning Electron

High resolution scanning electron microscopy of plant chromosomes

Chromosoma ◽  
1991 ◽  
Vol 100 (2) ◽  
pp. 103-109 ◽  
Author(s):  
G. Wanner ◽  
H. Formanek ◽  
R. Martin ◽  
R. G. Herrmann
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
High Resolution ◽  
Plant Chromosomes ◽  
Scanning Electron

Preparing plant chromosomes for scanning electron microscopy

Genome ◽  
1990 ◽  
Vol 33 (3) ◽  
pp. 333-339 ◽  
Author(s):  
John E. Dillé ◽  
Douglas C. Bittel ◽  
Kathleen Ross ◽  
J. Perry Gustafson
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
In Situ Hybridization ◽  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Light Microscopy ◽  
Plant Chromosomes ◽  
Cellular Debris ◽  
Scanning Electron

The scanning electron microscope may be useful in the analysis of plant chromosomes treated with in situ hybridization, especially when the probes and (or) chromosomes are near or beyond the resolution of the light microscope. Usual methods of plant chromosome preparation are unsuitable for scanning electron microscope observation as a result of cellular debris, which also interferes with probe hybridization. A method is described whereby protoplasts are obtained from fixed root tips by enzymatic digestion and applied to slides in a manner that produces little or no cellular debris overlying the chromosomes. The slides were examined by scanning electron microscopy and light microscopy after C-banding and in situ hybridization with a rye nucleolus organizer region spacer probe. This technique, which allows for scanning electron microscope visualization of bands and probes not easily identified with light microscopy, should prove useful in the physical mapping of low copy number or unique DNA sequences.Key words: protoplasts, rice, wheat, rye, physical maps, in situ hybridization.

Two- or three-way observations of the identical cell elements within the same sections by LM, TEM and/or SEM

1978 ◽  
Vol 36 (2) ◽  
pp. 82-83 ◽  
Author(s):  
Nakazo Watari ◽  
Yasuaki Hotta ◽  
Yoshio Mabuchi
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Fine Structure ◽  
Light Microscopy ◽  
Thin Sections ◽  
Transmission Electron ◽  
Identical Cell ◽  
Electron Microscopes ◽  
Scanning Electron

It is very useful if we can observe the identical cell elements within the same sections by light microscopy (LM), transmission electron microscopy (TEM) and/or scanning electron microscopy (SEM) sequentially, because, the cell fine structure can not be indicated by LM, while the color is; on the other hand, the cell fine structure can be very easily observed by EM, although its color properties may not. However, there is one problem in that LM requires thick sections of over 1 μm, while EM needs very thin sections of under 100 nm. Recently, we have developed a new method to observe the same cell elements within the same plastic sections using both light and transmission (conventional or high-voltage) electron microscopes.In this paper, we have developed two new observation methods for the identical cell elements within the same sections, both plastic-embedded and paraffin-embedded, using light microscopy, transmission electron microscopy and/or scanning electron microscopy (Fig. 1).

Sign in / Sign up

Export Citation Format

Share Document