Growth and Characterization of Cell and Tissue Cultures for the Study of Drug Transport

1991 ◽  
pp. 15-25
Author(s):  
Glynn Wilson
Keyword(s):  
Drug Transport ◽  
Tissue Cultures

scholarly journals Characterization of Rhodamine-123 as a Tracer Dye for Use In In vitro Drug Transport Assays

PLoS ONE ◽  
2012 ◽  
Vol 7 (3) ◽  
pp. e33253 ◽  
Author(s):  
Samantha Forster ◽  
Alfred E. Thumser ◽  
Steve R. Hood ◽  
Nick Plant
Keyword(s):  
Drug Transport ◽  
Rhodamine 123

Microstructured scaffolds for liver tissue cultures of high cell density: Morphological and biochemical characterization of tissue aggregates

2005 ◽  
Vol 95 (2) ◽  
pp. 243-255 ◽  
Author(s):  
Erik Eschbach ◽  
Shyam S. Chatterjee ◽  
Michael Nöldner ◽  
Eric Gottwald ◽  
Hermann Dertinger ◽  
...  
Keyword(s):  
Cell Density ◽  
Liver Tissue ◽  
Biochemical Characterization ◽  
High Cell Density ◽  
Tissue Cultures ◽  
High Cell

scholarly journals Characterization of the Human Intestinal Drug Transport with Ussing Chamber System Incorporating Freshly Isolated Human Jejunum

2020 ◽  
Vol 49 (1) ◽  
pp. 84-93
Author(s):  
Kazuyoshi Michiba ◽  
Kazuya Maeda ◽  
Ko Kurimori ◽  
Tsuyoshi Enomoto ◽  
Osamu Shimomura ◽  
...  
Keyword(s):  
Drug Transport ◽  
Ussing Chamber ◽  
Chamber System ◽  
Intestinal Drug Transport

scholarly journals TENDOVAGINITIS WITH ARTHRITIS, A NEW SYNDROME OF CHICKENS: ISOLATION AND CHARACTERIZATION OF AN INFECTIOUS AGENT

1955 ◽  
Vol 102 (4) ◽  
pp. 489-498 ◽  
Author(s):  
J. G. Lecce ◽  
F. G. Sperling ◽  
L. Hayflick ◽  
W. Stinebring
Keyword(s):  
Antibiotic Sensitivity ◽  
Infectious Agent ◽  
Tissue Cultures ◽  
Tetracycline Antibiotics ◽  
Isolation And Characterization ◽  
Sensitivity Tests ◽  
Large Virus ◽  
Chick Heart ◽  
Cytopathogenic Effect

The findings with an infectious agent isolated from cases of tendovaginitis or arthritis of chickens were as follows:— Cultures, stains, and darkfield studies of material containing this agent for spirochetes, bacteria, and pleuropneumonia-like organisms were negative. The yolk sac was the preferred route of inoculation, all of the embryos dying in 4 to 12 days. The agent passed through a bacteria-retaining filter, but with a drop in titer. Antibiotic sensitivity tests revealed that the agent was most sensitive to the tetracycline antibiotics, less sensitive to chloromycetin, dihydrostreptomycin, and resistant to penicillin. From electron micrographs it was concluded that the agent is a rigid, dense, coccobacillus from 0.2 µ to 0.5 µ, in size. The agent produced a cytopathogenic effect in tissue cultures of chick heart fibroblasts. The agent had no gross effect on suckling or newly weaned mice, pigs, or guinea pigs. Reasons for suggesting that the agent is most similar to a rickettsia or possibly a large virus are discussed.

scholarly journals CHARACTERIZATION OF A FACTOR FORMED IN THE COURSE OF ADENOVIRUS INFECTION OF TISSUE CULTURES CAUSING DETACHMENT OF CELLS FROM GLASS

1958 ◽  
Vol 108 (5) ◽  
pp. 713-729 ◽  
Author(s):  
Wallace P. Rowe ◽  
Janet W. Hartley ◽  
Bernard Roizman ◽  
Hilton B. Levy
Keyword(s):  
Tissue Culture ◽  
Infectious Virus ◽  
Neutralizing Antibody ◽  
Cell Detachment ◽  
Tissue Cultures ◽  
Kb Cells ◽  
Temperature Dependent ◽  
Reproducible Method ◽  
Glass Surfaces

Infectious tissue culture fluids of the majority of serotypes of adenovirus at low dilutions detach HeLa or KB cells from glass surfaces within a few hours after inoculation. A reproducible method for testing cell detachment was devised. The factor present in infectious tissue culture fluids and responsible for cell detachment is trypsin-sensitive and non-dialyzable; it is smaller and more resistant to the effect of heat or ultraviolet light than the infectious virus particle. Cell detachment activity was found to be temperature-dependent, and the cell-detaching titer of infectious tissue culture fluids was not affected by repeated exposure to HeLa cells. Inhibition of cell detachment by human or rabbit sera was observed only when other antibodies to adenovirus antigens were also present, but the antibody inhibiting cell detachment could not be correlated quantitatively with complement-fixing or homologous neutralizing antibody.

scholarly journals Identification of Six Putative Novel Human Papillomaviruses (HPV) and Characterization of Candidate HPV Type 87

2001 ◽  
Vol 75 (23) ◽  
pp. 11913-11919 ◽  
Author(s):  
Stefano Menzo ◽  
Alessia Monachetti ◽  
Caterina Trozzi ◽  
Andrea Ciavattini ◽  
Guido Carloni ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Biological Data ◽  
Human Papillomaviruses ◽  
The Novel ◽  
Tissue Cultures ◽  
Coding Region ◽  
Coding Regions ◽  
Immunodeficiency Virus ◽  
E6 And E7

ABSTRACT Six putative novel human papillomavirus (HPV) types were detected by using general primers for a conserved L1 HPV region in patients examined in gynecologic centers. One of the isolates, detected in samples from 4 patients with koilocytic atypia at cervical cytology (3 of whom were also infected with human immunodeficiency virus type 1), was completely sequenced, identified as a new HPV genotype, and designated candidate HPV87 (candHPV87) by the Reference Center for Human Papillomavirus. candHPV87 shows the classic HPV genome organization and the absence of a functional E5 coding region. Phylogenetic analysis documented that thecandHPV87 genome clusters within the A3 group of HPVs, together with HPV61, HPV72, HPV83, HPV84 and candHPV86, which have been completely sequenced, and a number of other putative novel genotypes (two of which are described in this work), which have been partially characterized. To address the growth-enhancing potential of candHPV87, the E6 and E7 putative coding regions were cloned and expressed in tissue cultures. The data indicate that both proteins stimulate cell division in tissue cultures more than those of low-risk HPVs, though not as much as those of HPV16. Taken together, the clinical, molecular, and biological data suggest that the novel papillomavirus characterized in the present study is a low- to intermediate-risk HPV.

scholarly journals FURTHER CHARACTERIZATION OF THE ADENOVIRUS ERYTHROCYTE RECEPTOR-MODIFYING FACTOR

1961 ◽  
Vol 114 (5) ◽  
pp. 717-728 ◽  
Author(s):  
Julius A. Kasel ◽  
Wallace P. Rowe ◽  
John L. Nemes
Keyword(s):  
Infectious Virus ◽  
Rabbit Antiserum ◽  
Human Erythrocytes ◽  
Red Cells ◽  
Tissue Cultures ◽  
Test Virus

Agglutinability of human erythrocytes for 3 hemagglutinating adenoviruses was markedly reduced by pretreatment of red cells with a factor present in tissue cultures which had been infected with adenovirus types 1, 2,4, or 15. The factor responsible for erythrocyte receptor modification was non-dialyzable and unaffected by the action of ribonuclease, desoxyribonuclease, trypsin, chymotrypsin, or ether. The factor was smaller, more thermostable, and separable from the infectious virus. Erythrocyte receptor modification was found to be a function of time and temperature. Titers of erythrocyte receptor-modifying activity were not diminished by successive exposures to fresh erythrocytes. Erythrocytes treated with erythrocyte receptor-modifying factor suspensions failed to significantly adsorb test virus hemagglutinin. Inhibition of erythrocyte receptor modifying-activity of the adenovirus suspensions by rabbit antiserum was type-specific.

scholarly journals Formulation, optimization and characterization of Betaxolol hydrochloride proniosomes using 3-2 factorial design

2020 ◽  
Vol 1 (3) ◽  
pp. 89-97
Author(s):  
V. Viswanath ◽  
P. Tulasi
Keyword(s):  
Factorial Design ◽  
Drug Transport ◽  
Liquid Crystalline ◽  
Diffusion Mechanism ◽  
Optimization Technique ◽  
Independent Variables ◽  
Drug Content ◽  
Dependent Variables ◽  
Span 60

The revolution in nanotechnology has lead to the development of various dosage forms such as vesicular drug delivery and in particular liposomes, niosomes, proniosomes, aquasomes, bilosomes etc. The disad-vantages exhibited by the liposomes, niosomes can be overcome through introduction of proniosomes which are compact liquid crystalline structures and convert to niosomes upon hydration. The investigation is focused on development and optimization of Betaxolol proniosomes using three square factorial design technique with the aid of design expert 11.0 ® trial version. The optimization technique prefers choles-terol and span 60 as independent variables and drug content, vesicular size, and entrapment efficacy as dependent variables. The design generated total 13 formulations among which F10 exhibited 98.1% drug content and 97.3% of entrapment efficacy. In view of other parameters, F10 exhibits 6.5 pH, 3.8 ve-sicular size and follows diffusion mechanism with anomalous drug transport. Hence, the obtained results specify that F10 is optimized and can be opted for commercialization.

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