scholarly journals FURTHER CHARACTERIZATION OF THE ADENOVIRUS ERYTHROCYTE RECEPTOR-MODIFYING FACTOR

1961 ◽  
Vol 114 (5) ◽  
pp. 717-728 ◽  
Author(s):  
Julius A. Kasel ◽  
Wallace P. Rowe ◽  
John L. Nemes
Keyword(s):  
Infectious Virus ◽  
Rabbit Antiserum ◽  
Human Erythrocytes ◽  
Red Cells ◽  
Tissue Cultures ◽  
Test Virus

Agglutinability of human erythrocytes for 3 hemagglutinating adenoviruses was markedly reduced by pretreatment of red cells with a factor present in tissue cultures which had been infected with adenovirus types 1, 2,4, or 15. The factor responsible for erythrocyte receptor modification was non-dialyzable and unaffected by the action of ribonuclease, desoxyribonuclease, trypsin, chymotrypsin, or ether. The factor was smaller, more thermostable, and separable from the infectious virus. Erythrocyte receptor modification was found to be a function of time and temperature. Titers of erythrocyte receptor-modifying activity were not diminished by successive exposures to fresh erythrocytes. Erythrocytes treated with erythrocyte receptor-modifying factor suspensions failed to significantly adsorb test virus hemagglutinin. Inhibition of erythrocyte receptor modifying-activity of the adenovirus suspensions by rabbit antiserum was type-specific.

scholarly journals CHARACTERIZATION OF A FACTOR FORMED IN THE COURSE OF ADENOVIRUS INFECTION OF TISSUE CULTURES CAUSING DETACHMENT OF CELLS FROM GLASS

1958 ◽  
Vol 108 (5) ◽  
pp. 713-729 ◽  
Author(s):  
Wallace P. Rowe ◽  
Janet W. Hartley ◽  
Bernard Roizman ◽  
Hilton B. Levy
Keyword(s):  
Tissue Culture ◽  
Infectious Virus ◽  
Neutralizing Antibody ◽  
Cell Detachment ◽  
Tissue Cultures ◽  
Kb Cells ◽  
Temperature Dependent ◽  
Reproducible Method ◽  
Glass Surfaces

Infectious tissue culture fluids of the majority of serotypes of adenovirus at low dilutions detach HeLa or KB cells from glass surfaces within a few hours after inoculation. A reproducible method for testing cell detachment was devised. The factor present in infectious tissue culture fluids and responsible for cell detachment is trypsin-sensitive and non-dialyzable; it is smaller and more resistant to the effect of heat or ultraviolet light than the infectious virus particle. Cell detachment activity was found to be temperature-dependent, and the cell-detaching titer of infectious tissue culture fluids was not affected by repeated exposure to HeLa cells. Inhibition of cell detachment by human or rabbit sera was observed only when other antibodies to adenovirus antigens were also present, but the antibody inhibiting cell detachment could not be correlated quantitatively with complement-fixing or homologous neutralizing antibody.

scholarly journals Modelling Degradation and Replication Kinetics of the Zika Virus In Vitro Infection

Viruses ◽  
2020 ◽  
Vol 12 (5) ◽  
pp. 547
Author(s):  
Veronika Bernhauerová ◽  
Veronica V. Rezelj ◽  
Marco Vignuzzi
Keyword(s):  
Mathematical Model ◽  
Viral Infection ◽  
Host Cell ◽  
Decay Time ◽  
Zika Virus ◽  
Infectious Virus ◽  
Numerical Characterization ◽  
The Mathematical Model

Mathematical models of in vitro viral kinetics help us understand and quantify the main determinants underlying the virus–host cell interactions. We aimed to provide a numerical characterization of the Zika virus (ZIKV) in vitro infection kinetics, an arthropod-borne emerging virus that has gained public recognition due to its association with microcephaly in newborns. The mathematical model of in vitro viral infection typically assumes that degradation of extracellular infectious virus proceeds in an exponential manner, that is, each viral particle has the same probability of losing infectivity at any given time. We incubated ZIKV stock in the cell culture media and sampled with high frequency for quantification over the course of 96 h. The data showed a delay in the virus degradation in the first 24 h followed by a decline, which could not be captured by the model with exponentially distributed decay time of infectious virus. Thus, we proposed a model, in which inactivation of infectious ZIKV is gamma distributed and fit the model to the temporal measurements of infectious virus remaining in the media. The model was able to reproduce the data well and yielded the decay time of infectious ZIKV to be 40 h. We studied the in vitro ZIKV infection kinetics by conducting cell infection at two distinct multiplicity of infection and measuring viral loads over time. We fit the mathematical model of in vitro viral infection with gamma distributed degradation time of infectious virus to the viral growth data and identified the timespans and rates involved within the ZIKV-host cell interplay. Our mathematical analysis combined with the data provides a well-described example of non-exponential viral decay dynamics and presents numerical characterization of in vitro infection with ZIKV.

Characterization of the glucose transporter from human erythrocytes

1978 ◽  
Vol 8 (4) ◽  
pp. 447-453 ◽  
Author(s):  
David C. Sogin ◽  
Peter C. Hinkle
Keyword(s):  
Glucose Transporter ◽  
Human Erythrocytes

Characterization of the intermediate (10 nm) filaments of cultured cells using an autoimmune rabbit antiserum

Cell ◽  
1978 ◽  
Vol 13 (2) ◽  
pp. 249-261 ◽  
Author(s):  
William E. Gordon ◽  
Anne Bushnell ◽  
Keith Burridge
Keyword(s):  
Cultured Cells ◽  
Rabbit Antiserum ◽  
10 Nm Filaments

scholarly journals Thrombospondin mediates the cytoadherence of Plasmodium falciparum- infected red cells to vascular endothelium in shear flow conditions

Blood ◽  
1988 ◽  
Vol 71 (1) ◽  
pp. 71-75
Author(s):  
EP Rock ◽  
EF Jr Roth ◽  
RR Rojas-Corona ◽  
JA Sherwood ◽  
RL Nagel ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Cerebral Malaria ◽  
Ex Vivo ◽  
Rabbit Antiserum ◽  
Immune Serum ◽  
Red Cells ◽  
Specific Attachment ◽  
Infected Cells ◽  
Human Immune ◽  
Venular Endothelium

Cerebral malaria is thought to involve specific attachment of Plasmodium falciparum-infected knobby red cells to venular endothelium. The nature of surface ligands on host endothelial cells that may mediate cytoadherence is poorly understood. We have investigated the effects of soluble thrombospondin, rabbit antiserum raised against thrombospondin, and human immune serum on cytoadherence of parasitized erythrocytes in ex vivo mesocecum vasculature. Preincubation of infected red cells with soluble thrombospondin or human immune serum inhibits binding of infected red cells to rat venular endothelium. Infusion of the microcirculatory preparation with rabbit antithrombospondin antibodies before perfusion of parasitized erythrocytes also resulted in decreased cytoadherence. In addition, incubation of infected cells with human immune sera obtained from malaria patients significantly inhibited the observed cytoadherence. Our results indicate that thrombospondin mediates binding of infected red cells to venular endothelium and may thus be involved in the pathogenesis of cerebral malaria.

Purification and characterization of esterases D1 and D2 from human erythrocytes. Evidence that they are monomers

1985 ◽  
Vol 153 (2) ◽  
pp. 217-222 ◽  
Author(s):  
Kiyosato MATSUO ◽  
Kunihiko KOBAYASHI ◽  
Keiji HAGIWARA ◽  
Tadashi KAJII
Keyword(s):  
Human Erythrocytes ◽  
Purification And Characterization

scholarly journals Characterization of an MLP Homologue from Haemaphysalis longicornis (Acari: Ixodidae) Ticks

Pathogens ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 284
Author(s):  
Jin Luo ◽  
Hui Shen ◽  
Qiaoyun Ren ◽  
Guiquan Guan ◽  
Bo Zhao ◽  
...  
Keyword(s):  
Muscle Development ◽  
Developmental Stages ◽  
Rabbit Antiserum ◽  
Haemaphysalis Longicornis ◽  
Mrna Levels ◽  
Muscle Lim Protein ◽  
Rapid Amplification ◽  
Differentiation And Proliferation ◽  
Phosphate Buffered Saline

Members of the cysteine-rich protein (CRP) family are known to participate in muscle development in vertebrates. Muscle LIM protein (MLP) belongs to the CRP family and has an important function in the differentiation and proliferation of muscle cells. In this study, the full-length cDNA encoding MLP from Haemaphysalis longicornis (H. longicornis; HLMLP) ticks was obtained by 5′ rapid amplification of cDNA ends (RACE). To verify the transcriptional status of MLP in ticks, HLMLP gene expression was assessed during various developmental stages by real-time PCR (RT-PCR). Interestingly, HLMLP expression in the integument was significantly (P < 0.01) higher than that observed in other tested tissues of engorged adult ticks. In addition, HLMLP mRNA levels were significantly downregulated in response to thermal stress at 4 °C for 48 h. Furthermore, recombinant HLMLP was expressed in Escherichia coli, and Western blot analysis showed that rabbit antiserum against H. longicornis adults recognized HLMLP and MLPs from different ticks. Ten 3-month-old rabbits that had never been exposed to ticks were used for the immunization and challenge experiments. The rabbits were divided into two groups of five rabbits each, where rabbits in the first group were immunized with HLMLP, while those in the second group were immunized with phosphate-buffered saline (PBS) diluent as controls. The vaccination of rabbits with the recombinant HLMLP conferred partial protective immunity against ticks, resulting in 20.00% mortality and a 17.44% reduction in the engorgement weight of adult ticks. These results suggest that HLMLP is not ideal as a candidate for use in anti-tick vaccines. However, the results of this study generated novel information on the MLP gene in H. longicornis and provide a basis for further investigation of the function of this gene that could potentially lead to a better understanding of the mechanism of myofiber determination and transformation

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