Immunoregulation by Lymphokines: Immune Interferon and Lymphotoxin Induction of Lymphokine Activity in Human Peripheral Blood Leukocyte Cultures

1983 ◽  
pp. 37-44 ◽  
Author(s):  
C. H. Robbins
Keyword(s):  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Peripheral Blood Leukocyte ◽  
Immune Interferon ◽  
Blood Leukocyte

scholarly journals Immunological imprint of COVID‐19 on human peripheral blood leukocyte populations

Allergy ◽  
2020 ◽  
Author(s):  
Bernhard Kratzer ◽  
Doris Trapin ◽  
Paul Ettel ◽  
Ulrike Körmöczi ◽  
Arno Rottal ◽  
...  
Keyword(s):  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Peripheral Blood Leukocyte ◽  
Blood Leukocyte ◽  
Leukocyte Populations

HUMAN PERIPHERAL BLOOD LEUKOCYTE-RECONSTITUTED SEVERE COMBINED IMMUNODEFICIENT MOUSE

1997 ◽  
Vol 63 (6) ◽  
pp. 818-823 ◽  
Author(s):  
Ryochi Shiroki ◽  
Bashoo Naziruddin ◽  
Seiichiro Shishido ◽  
Brian F. Duffy ◽  
Todd Howard ◽  
...  
Keyword(s):  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Peripheral Blood Leukocyte ◽  
Immunodeficient Mouse ◽  
Blood Leukocyte

Sequential production of cytokines by dengue virus-infected human peripheral blood leukocyte cultures

1999 ◽  
Vol 59 (3) ◽  
pp. 335-340 ◽  
Author(s):  
U.C. Chaturvedi ◽  
E.A. Elbishbishi ◽  
R. Agarwal ◽  
R. Raghupathy ◽  
R. Nagar ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Peripheral Blood Leukocyte ◽  
Blood Leukocyte

scholarly journals Cultured human monocytes synthesize and secrete alpha2-macroglobulin.

1977 ◽  
Vol 145 (6) ◽  
pp. 1580-1589 ◽  
Author(s):  
T Hovi ◽  
D Mosher ◽  
A Vaheri
Keyword(s):  
Peripheral Blood ◽  
De Novo ◽  
Human Peripheral Blood ◽  
Polyacrylamide Gel Electrophoresis ◽  
Double Diffusion ◽  
Peripheral Blood Leukocyte ◽  
Mononuclear Leukocytes ◽  
Mononuclear Leukocyte ◽  
Peripheral Blood Mononuclear ◽  
Blood Leukocyte

alpha2-Macroglobulin levels in the supernates of cultures of different subpopulations of human peripheral blood mononuclear leukocytes were assayed by a radioimmunoassay. Unfractionated mononuclear leukocytes produced greater amounts of the macroglobulin (4.0 vs. 0.8 ng/10(6) cells) than did subpopulations enriched in T or B+T lymphocytes, by passage through nylon wool or cotton wool columns, respectively. Still higher concentrations of alpha2-macroglobulin (40 ng/10(6) cells) were measured in the supernates of glass-adherent mononuclear leukocyte cultures. These results suggest that cells of monocyte-macrophage lineage are mainly, if not exclusively, responsible for the appearance of alpha2- macroglobulin in the supernate of human peripheral blood leukocyte cultures. The de novo synthesis and release of alpha2-macroglobulin by cultured monocytes was demonstrated by immunoprecipitation of radioactivity from supernates of 32S-methionine-labeled glass-adherent cells. Antiserum against purified alpha2-macroglobulin was used in both Ouchterlony double diffusion and double antibody precipitation tests. SDS-polyacrylamide gel electrophoresis of immunoprecipitates showed that most of the radioactivity comigrated with authentic alpha2-macroglobulin subunit at about 160,000 daltons.

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