scholarly journals Cultured human monocytes synthesize and secrete alpha2-macroglobulin.

1977 ◽  
Vol 145 (6) ◽  
pp. 1580-1589 ◽  
Author(s):  
T Hovi ◽  
D Mosher ◽  
A Vaheri
Keyword(s):  
Peripheral Blood ◽  
De Novo ◽  
Human Peripheral Blood ◽  
Polyacrylamide Gel Electrophoresis ◽  
Double Diffusion ◽  
Peripheral Blood Leukocyte ◽  
Mononuclear Leukocytes ◽  
Mononuclear Leukocyte ◽  
Peripheral Blood Mononuclear ◽  
Blood Leukocyte

alpha2-Macroglobulin levels in the supernates of cultures of different subpopulations of human peripheral blood mononuclear leukocytes were assayed by a radioimmunoassay. Unfractionated mononuclear leukocytes produced greater amounts of the macroglobulin (4.0 vs. 0.8 ng/10(6) cells) than did subpopulations enriched in T or B+T lymphocytes, by passage through nylon wool or cotton wool columns, respectively. Still higher concentrations of alpha2-macroglobulin (40 ng/10(6) cells) were measured in the supernates of glass-adherent mononuclear leukocyte cultures. These results suggest that cells of monocyte-macrophage lineage are mainly, if not exclusively, responsible for the appearance of alpha2- macroglobulin in the supernate of human peripheral blood leukocyte cultures. The de novo synthesis and release of alpha2-macroglobulin by cultured monocytes was demonstrated by immunoprecipitation of radioactivity from supernates of 32S-methionine-labeled glass-adherent cells. Antiserum against purified alpha2-macroglobulin was used in both Ouchterlony double diffusion and double antibody precipitation tests. SDS-polyacrylamide gel electrophoresis of immunoprecipitates showed that most of the radioactivity comigrated with authentic alpha2-macroglobulin subunit at about 160,000 daltons.

scholarly journals Avidin inhibits PHA-induced human peripheral blood mononuclear cell proliferation

2016 ◽  
Vol 25 (1) ◽  
pp. 19-24
Author(s):  
Cicia Firakania ◽  
Indra G. Mansur ◽  
Sri W.A. Jusman ◽  
Mohamad Sadikin
Keyword(s):  
Cell Cycle ◽  
Cell Proliferation ◽  
Peripheral Blood Mononuclear Cell ◽  
Mononuclear Cell ◽  
Peripheral Blood ◽  
De Novo ◽  
Human Peripheral Blood ◽  
Interleukin 2 ◽  
Peripheral Blood Mononuclear ◽  
Cell Proliferation Inhibition

Background: Cell proliferation occurs not only in normal but also in cancer cells. Most of cell proliferation inhibition can be done by inhibiting the DNA synthesis, notably by intervening the formation of purine or pyrimidine. In purine de novo synthesis, it was assumed that biotin plays a role as a coenzyme in carboxylation reaction, one of the pivotal steps in the purine de novo pathways. The aim of this study was to see the avidin potency to bind biotin and inhibit mitosis.Methods: Peripheral blood mononuclear cell (PBMC) was cultured in RPMI-1640 medium and stimulated by phytohemagglutinin (PHA) in the presence or absence of interleukin-2 (IL-2), with or without avidin. The effect of avidin addition was observed at 24, 48, and 72 hours for cell proliferation, viability, and cell cycle. Statistical analysis was done by one-way ANOVA.Results: Avidin inhibited cell proliferation and viability in culture under stimulation by PHA with and without IL-2. Cell cycle analysis showed that avidin arrested the progression of PBMC after 72 hours of culture. Most cells were found in G0/G1 phase.Conclusion: Inhibition of biotin utilization by avidin binding can halt cell proliferation.

scholarly journals Immunological imprint of COVID‐19 on human peripheral blood leukocyte populations

Allergy ◽  
2020 ◽  
Author(s):  
Bernhard Kratzer ◽  
Doris Trapin ◽  
Paul Ettel ◽  
Ulrike Körmöczi ◽  
Arno Rottal ◽  
...  
Keyword(s):  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Peripheral Blood Leukocyte ◽  
Blood Leukocyte ◽  
Leukocyte Populations

HUMAN PERIPHERAL BLOOD LEUKOCYTE-RECONSTITUTED SEVERE COMBINED IMMUNODEFICIENT MOUSE

1997 ◽  
Vol 63 (6) ◽  
pp. 818-823 ◽  
Author(s):  
Ryochi Shiroki ◽  
Bashoo Naziruddin ◽  
Seiichiro Shishido ◽  
Brian F. Duffy ◽  
Todd Howard ◽  
...  
Keyword(s):  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Peripheral Blood Leukocyte ◽  
Immunodeficient Mouse ◽  
Blood Leukocyte
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