Optical Imaging and In Vivo Microscopy

2001 ◽  
pp. 260-271
Author(s):  
Zuxing Kan ◽  
E. Edmund Kim
Keyword(s):  
Optical Imaging ◽  
In Vivo Microscopy

Optical imaging probes and their potential contribution to radiotracer development

2016 ◽  
Vol 55 (02) ◽  
pp. 51-62 ◽  
Author(s):  
S. Hermann ◽  
M. Schäfers ◽  
C. Höltke ◽  
A. Faust
Keyword(s):  
Optical Imaging ◽  
Fluorescent Dyes ◽  
Great Influence ◽  
Potential Contribution ◽  
Preclinical Evaluation ◽  
Guided Surgery ◽  
Fluorescence Guided Surgery ◽  
Imaging Probes ◽  
Unspecific Binding

SummaryOptical imaging has long been considered a method for histological or microscopic investigations. Over the last 15 years, however, this method was applied for preclinical molecular imaging and, just recently, was also able to show its principal potential for clinical applications (e.g. fluorescence-guided surgery). Reviewing the development and preclinical evaluation of new fluorescent dyes and target-specific dye conjugates, these often show characteristic patterns of their routes of excretion and biodistribution, which could also be interesting for the development and optimization of radiopharmaceuticals. Especially ionic charges show a great influence on biodistribution and netcharge and charge-distribution on a conjugate often determines unspecific binding or background signals in liver, kidney or intestine, and other organs.Learning from fluorescent probe behaviour in vivo and translating this knowledge to radio-pharmaceuticals might be useful to further optimize emerging and existing radiopharmaceuticals with respect to their biodistribution and thereby availability for binding to their targets.

Studies on Chick Embryo Thrombocytes

1963 ◽  
Vol 09 (02) ◽  
pp. 291-299 ◽  
Author(s):  
Helge Stalsberg ◽  
Hans Prydz
Keyword(s):  
Chick Embryo ◽  
Bleeding Time ◽  
In Vivo Microscopy ◽  
Cut Edge ◽  
Vessel Lumen ◽  
Cellular Constituent

SummaryThe formation of hemostatic plugs were studied in the chick embryo through in vivo microscopy, in sections of hemostatic plugs and by measurements of primary bleeding time. Thrombocytes were found to be their only cellular constituent. Ability to form adequate hemostatic plugs appeared rather abruptly in embryos of stages 16-17 and coincided with an increase in thrombocyte precursors (stages III and IV).The thrombocytes initially adhere to the cut edge of the vessel. The extension of the hemostatic plug into the vessel lumen is a secondary step in plug development.

scholarly journals Micelle-based activatable probe for in vivo near-infrared optical imaging of cancer biomolecules

2014 ◽  
Vol 10 (1) ◽  
pp. 187-195 ◽  
Author(s):  
Yoichi Shimizu ◽  
Takashi Temma ◽  
Isao Hara ◽  
Akira Makino ◽  
Ryo Yamahara ◽  
...  
Keyword(s):  
Optical Imaging ◽  
Near Infrared ◽  
Activatable Probe

scholarly journals Therapeutic Effects of Targeted PPARɣ Activation on Inflamed High-Risk Plaques Assessed by Serial Optical Imaging In Vivo

Theranostics ◽  
2018 ◽  
Vol 8 (1) ◽  
pp. 45-60 ◽  
Author(s):  
Jah Yeon Choi ◽  
Jiheun Ryu ◽  
Hyun Jung Kim ◽  
Joon Woo Song ◽  
Joo Hee Jeon ◽  
...  
Keyword(s):  
High Risk ◽  
Optical Imaging ◽  
Therapeutic Effects

In Vivo Microscopy Using the Tandem Scanning Microscope

1986 ◽  
Vol 483 (1 Recent Advanc) ◽  
pp. 440-447 ◽  
Author(s):  
M. PETRAN ◽  
M. HADRAVSKY ◽  
J. BENES ◽  
A. BOYDE
Keyword(s):  
In Vivo Microscopy ◽  
Scanning Microscope

scholarly journals Assessment of Endothelin-A Receptor Expression in Subcutaneous and Orthotopic Thyroid Carcinoma Xenografts in Vivo Employing Optical Imaging Methods

Endocrinology ◽  
2012 ◽  
Vol 153 (6) ◽  
pp. 2907-2918 ◽  
Author(s):  
Katrin Büther ◽  
Matthijs G. Compeer ◽  
Jo G. R. De Mey ◽  
Otmar Schober ◽  
Michael Schäfers ◽  
...  
Keyword(s):  
Thyroid Carcinoma ◽  
Optical Imaging ◽  
Near Infrared ◽  
Functional Performance ◽  
Thyroid Tumor ◽  
Receptor Expression ◽  
Imaging Methods ◽  
Carcinoma Cell Lines ◽  
Isolated Artery

Endothelin (ET) receptor dysregulation has been described in a number of pathophysiological processes, including cardiovascular disorders, renal failure, and cancer. The aim of this study was to evaluate the expression of the ET-A receptor (ETAR) in murine models of thyroid carcinoma using optical imaging methods. A recently developed near-infrared fluorescent tracer was first assessed in isolated artery preparations for its functional performance in comparison with known ETAR antagonists BQ123 and PD156707. Before evaluation of the tracer in vivo, different thyroid carcinoma cell lines were characterized with respect to their ET receptor expression by RT-PCR and autoradiography. In vivo, sc and orthotopic papillary thyroid tumor xenografts were clearly visualized by fluorescence reflectance imaging and fluorescence-mediated tomography up to 48 h after injection of the tracer. Binding specificity of the probe was demonstrated by predosing with PD156707 as a competing inhibitor. In conclusion, optical imaging with a fluorescent ETAR tracer allows the noninvasive imaging of tumor-associated ETAR expression in vivo. In the future, this technique may help surgeons to evaluate lesion dimensions in intraoperative settings (e.g. thyroidectomy).

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