Optical imaging using an Hsp70 NIR probe to monitor the therapeutic efficiency of hyperthermia in vitro and in vivo

2015 ◽  
Vol 187 (S 01) ◽  
Author(s):  
J Grandke ◽  
S Rosenhain ◽  
U Teichgräber ◽  
I Hilger
Keyword(s):  
Optical Imaging ◽  
Therapeutic Efficiency

scholarly journals Optical imaging-guided cancer therapy with fluorescent nanoparticles

2009 ◽  
Vol 7 (42) ◽  
pp. 3-18 ◽  
Author(s):  
Shan Jiang ◽  
Muthu Kumara Gnanasammandhan ◽  
Yong Zhang
Keyword(s):  
Cancer Therapy ◽  
Optical Imaging ◽  
Critical Role ◽  
Therapeutic Agents ◽  
Fluorescent Nanoparticles ◽  
Highly Sensitive ◽  
Imaging Probes ◽  
Recent Developments

The diagnosis and treatment of cancer have been greatly improved with the recent developments in nanotechnology. One of the promising nanoscale tools for cancer diagnosis is fluorescent nanoparticles (NPs), such as organic dye-doped NPs, quantum dots and upconversion NPs that enable highly sensitive optical imaging of cancer at cellular and animal level. Furthermore, the emerging development of novel multi-functional NPs, which can be conjugated with several functional molecules simultaneously including targeting moieties, therapeutic agents and imaging probes, provides new potentials for clinical therapies and diagnostics and undoubtedly will play a critical role in cancer therapy. In this article, we review the types and characteristics of fluorescent NPs, in vitro and in vivo imaging of cancer using fluorescent NPs and multi-functional NPs for imaging-guided cancer therapy.

scholarly journals Fluorinated PLGA-PEG-Mannose Nanoparticles for Tumor-Associated Macrophage Detection by Optical Imaging and MRI

2021 ◽  
Vol 8 ◽  
Author(s):  
Giorgia Zambito ◽  
Siyuan Deng ◽  
Joost Haeck ◽  
Natasa Gaspar ◽  
Uwe Himmelreich ◽  
...  
Keyword(s):  
Magnetic Resonance ◽  
Optical Imaging ◽  
Tumor Development ◽  
Mannose Receptor ◽  
The Body ◽  
Mri Contrast Agent ◽  
Therapeutic Interventions ◽  
Magnetic Resonance Imaging Mri

Tumor-associated macrophages (TAMs) promote cancer growth and metastasis, but their role in tumor development needs to be fully understood due to the dynamic changes of tumor microenvironment (TME). Here, we report an approach to visualize TAMs by optical imaging and by Fluorine-19 (19F) magnetic resonance imaging (MRI) that is largely applied to track immune cells in vivo. TAMs are targeted with PLGA-PEG-mannose nanoparticles (NPs) encapsulating perfluoro-15-crown-5-ether (PFCE) as MRI contrast agent. These particles are preferentially recognized and phagocytized by TAMs that overexpress the mannose receptor (MRC1/CD206). The PLGA-PEG-mannose NPs are not toxic and they were up-taken by macrophages as confirmed by in vitro confocal microscopy. At 48 h after intravenous injection of PLGA-PEG-mannose NPs, 4T1 xenograft mice were imaged and fluorine-19 nuclear magnetic resonance confirmed nanoparticle retention at the tumor site. Because of the lack of 19F background in the body, observed 19F signals are robust and exhibit an excellent degree of specificity. In vivo imaging of TAMs in the TME by 19F MRI opens the possibility for detection of cancer at earlier stage and for prompt therapeutic interventions in solid tumors.

scholarly journals The Development and Applications of a Dual Optical Imaging System for Studying Glioma Stem Cells

2019 ◽  
Vol 18 ◽  
pp. 153601211987089 ◽  
Author(s):  
Po-An Tai ◽  
Yen-Lin Liu ◽  
Ya-Ting Wen ◽  
Chien-Min Lin ◽  
Thanh-Tuan Huynh ◽  
...  
Keyword(s):  
Stem Cells ◽  
Brain Tumor ◽  
Optical Imaging ◽  
Fluorescent Protein ◽  
Imaging System ◽  
High Rate ◽  
Glioma Stem Cells ◽  
Optical Imaging System

Glioblastoma multiforme represents one of the deadliest brain tumor types, manifested by a high rate of recurrence and poor prognosis. The presence of glioma stem cells (GSCs) can repopulate the tumor posttreatment and resist therapeutics. A better understanding of GSC biology is essential for developing more effective interventions. We established a CD133 promoter-driven dual reporter, expressing green fluorescent protein (GFP) and firefly luciferase (CD133-LG), capable for in vitro and in vivo imaging of CD133+ GSCs. We first demonstrated the reporter enabled in vitro analyses of GSCs. DBTRG-05MG (Denver Brain Tumor Research Group 05) carrying CD133-LG (DBTRG-05MG-CD133-LG) system reported increased GFP/luciferase activities in neurospheres. Additionally, we identified and isolated CD133+/GFP+ cells with increased tumorigenic properties, stemness markers, Notch1, β-catenin, and Bruton’s tyrosine kinase (Btk). Furthermore, prolonged temozolomide (TMZ) treatment enriched GSCs (reflected by increased percentage of CD133+ cells). Subsequently, Btk inhibitor, ibrutinib, suppressed GSC generation and stemness markers. Finally, we demonstrated real-time evaluation of anti-GSC function of ibrutinib in vivo with TMZ-enriched GSCs. Tumorigenesis was noninvasively monitored by bioluminescence imaging and mice that received ibrutinib showed a significantly lower tumor burden, indicating ibrutinib as a potential GSC inhibitor. In conclusion, we established a dual optical imaging system which enables the identification of CD133+ GSCs and screening for anti-GSC drugs.

In vitro and in vivo trackable titanocene-based complexes using optical imaging or SPECT

2017 ◽  
Vol 46 (42) ◽  
pp. 14548-14555 ◽  
Author(s):  
Océane Florès ◽  
Audrey Trommenschlager ◽  
Souheila Amor ◽  
Fernanda Marques ◽  
Francisco Silva ◽  
...  
Keyword(s):  
Optical Imaging

Two unprecedented titanocene-based theranostics have been synthesized, characterized, and tracked either in vitro (BODIPY probe) or in vivo (111In-DOTA probe).

CD44-overexpressed gastric cancer imaging by near IR using HA-based supramolecular hydrogels.

2014 ◽  
Vol 32 (3_suppl) ◽  
pp. 34-34
Author(s):  
Jungmin Park
Keyword(s):  
Gastric Cancer ◽  
Optical Imaging ◽  
Cancer Cells ◽  
Cancer Cell ◽  
Gastric Cancer Cell ◽  
Gastric Cancer Cell Line ◽  
Cancer Cell Line ◽  
Gastric Cancer Cells

34 Background: To establish NIR optical probe based on the HA-based supramolecular hydrogels (HASHs) conjugated with Cy5.5 for CD44-overexpressed gastric cancer imaging. Methods: To establish HASHs, Cy5.5 NHS ester was conjugated with polyethyleneimine (PEI, 25k Da) and mixed with hyaluronic acid (HA, 1M Da) by an electric interaction. The optimazed ideal molar ratio of PEI to HA was confirmed by DLS and gel electrophoresis. The CD44-expression level for various gastric cancer cell lInes (MKN1, MKN28, MKN45, MKN72, AGS, and N87 cells) was evaluated by FACS analysis. For establishment of the gastric cancer xenograft model, CD44-overexpressed gastric cancer cells were implanted into the BALB/c nude mouse's proximal thigh region. For in vitro targeting study, the cellular affinity of HASHs for CD44-low expressed gastric cancer cell line and CD44-overexpressed gastric cancer cell line was verified by confocal microscopy and IHC staing. For in vivo NIR imaging, HASHs were injected into established xenograft mouse via tail vein and NIR optical imaging was conducted time-dependently. Results: The colloidal size of HASHs was 1.4 micrometer and their morphology was confirmed by electron microscopy. CD44-expression level of MKN45 cells was 92.53% that was higher than MKN28 cells (2.66%). After the treatment of HASHs, the endocytosis into the cytosol was examined for MKN45 cells, but not observed in MKN28 cells due to the deficiency of CD44. 30 days after the transplantion of MKN45 cells, for in vivo imaging study, the prepared HASHs were intraveneously injected into tumor-bearing mouse model. By NIR optical imaging, the optical intensity at tumor site was enhanced upto 3 hours and the maximum intensity was 350 times larger than normal tissue. Conclusions: HASHs was established using supramolecular HA and Cy5.5-conjugated PEI for the targeted imaging of CD44-overexpressed gastric cancer cells. In vitro and in vivo studies demonstrated that SHAHs can visualize the individualized CD44-overexpressed gastric cancer cells by non-invasive optical imaging.

Synthesis, in vitro, and in vivo characterization of an integrin αvβ3-targeted molecular probe for optical imaging of tumor

2005 ◽  
Vol 13 (11) ◽  
pp. 3763-3771 ◽  
Author(s):  
Christopher A. Burnett ◽  
Jianwu Xie ◽  
Jade Quijano ◽  
Zhimin Shen ◽  
Finie Hunter ◽  
...  
Keyword(s):  
Optical Imaging ◽  
Integrin Αvβ3 ◽  
Molecular Probe ◽  
In Vivo Characterization

In vitro and In vivo Optical Imaging Using Water-Dispersible, Noncytotoxic, Luminescent, Silica-Coated Quantum Rods

2010 ◽  
Vol 22 (7) ◽  
pp. 2261-2267 ◽  
Author(s):  
Rajiv Kumar ◽  
Hong Ding ◽  
Rui Hu ◽  
Ken-Tye Yong ◽  
Indrajit Roy ◽  
...  
Keyword(s):  
Optical Imaging ◽  
Water Dispersible ◽  
Quantum Rods ◽  
In Vivo Optical Imaging

Water-soluble Zn–Ag–In–Se quantum dots with bright and widely tunable emission for biomedical optical imaging

RSC Advances ◽  
2015 ◽  
Vol 5 (108) ◽  
pp. 88583-88589 ◽  
Author(s):  
Jie Wang ◽  
Rong Zhang ◽  
Fangjian Bao ◽  
Zhihao Han ◽  
Yueqing Gu ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Optical Imaging ◽  
Water Soluble ◽  
Biomedical Optical Imaging ◽  
Tunable Emission

In this work, we synthesized water-soluble quaternary cadmium-free Zn–Ag–In–Se quantum dots with bright and widely tunable emission, and explored their potential in tumor-specific imaging in vitro and in vivo.

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