Cross-Reactivities and Strain-Specificity of Monoclonal Antibodies to Campylobacter Jejuni and Helicobacter Pylori Flagellins

Background: The updated model for the mechanism of gastric carcinogenesis demonstrates that Helicobacter pylori (H. pylori) is a risk factor in every step of the process. The expression of certain gastric mucins is altered by H. pylori infection in adult patients. The aim of our research was to assess the impact of H. pylori infection on the expression of secretory mucins in the pediatric antral mucosa. Methods: Slides were stained with monoclonal antibodies for MUC5AC, MUC6 and MUC2, digitalized and scored using both a semiquantitative and a quantitative approach. Results: The expression of MUC5AC was significantly lower in infected children. Also, MUC2 expression was more pronounced in infected children. MUC6 expression did not differentiate between infected and noninfected children. Additionally, the presence of chronic inflammation significantly altered the expression of MUC6 and MUC2. The expression of MUC6 was significantly higher in patients with gastric atrophy. Conclusion: The minor differences in mucin expression at distinct ages might stem from different H. pylori exposure periods. Further research is needed to determine the particular patterns of expression according to age and to evaluate the effects of the interaction between H. pylori and mucins in the progression of the gastric carcinogenesis cascade.

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LINFOMI ASSOCIATI ALL’INFEZIONE DA Helicobacter pylori, Borrelia burgdorferi e Campylobacter jejuni

Microbiologia Medica ◽

10.4081/mm.2005.3421 ◽

2005 ◽

Vol 20 (3) ◽

Author(s):

C. Doglioni

Keyword(s):

Helicobacter Pylori ◽

Borrelia Burgdorferi ◽

Campylobacter Jejuni

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The Role of Endotoxin in Infection: Helicobacter pylori and Campylobacter jejuni

Subcellular Biochemistry - Endotoxins: Structure, Function and Recognition ◽

10.1007/978-90-481-9078-2_10 ◽

2010 ◽

pp. 209-240 ◽

Cited By ~ 10

Author(s):

Anthony P. Moran

Keyword(s):

Helicobacter Pylori ◽

Campylobacter Jejuni

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Monoclonal antibodies directed against the flagella ofCampylobacter jejuni: cross-reacting and serotypic specificity and potential use in diagnosis

Journal of Hygiene ◽

10.1017/s0022172400066134 ◽

1986 ◽

Vol 96 (3) ◽

pp. 377-384 ◽

Cited By ~ 26

Author(s):

D. G. Newell

Keyword(s):

Monoclonal Antibody ◽

Monoclonal Antibodies ◽

Campylobacter Jejuni ◽

Clinical Material ◽

Faecal Material ◽

Ofcampylobacter Jejuni ◽

Elisa Technique ◽

Flagellar Antigen ◽

Potential Use

SUMMARYNine monoclonal antibodies directed against the flagella ofCampylobacter jejunistrain 81116 have been investigated for serotypic and cross-reacting activity using a panel of 17 Penner serotype strains ofC. jejuni.Four monoclonal antibodies were exclusively specific for serotype-6 strains, which was the serotype ofC. jejunistrain 81116. Two monoclonal antibodies cross-reacted with all the flagellated strains ofC jejunitested. One of these cross-reacting monoclonal antibodies, CF5, was found to react with all otherCampylobacterspecies exceptC. sputorum bulbulusbut it did not react with other bacterial entcropathogens. An antigencapture ELISA technique was established, using this monoclonal antibody, which could detect flagellar antigen in human faecal material. These anti-flagella monoclonal antibodies therefore may be valuable in the diagnosis and serotyping ofC. jejuniin clinical material.

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Production and characterisation of monoclonal antibodies to heat-shock protein 60 of Helicobacter pylori

Journal of Medical Microbiology ◽

10.1099/00222615-46-10-819 ◽

1997 ◽

Vol 46 (10) ◽

pp. 819-824 ◽

Cited By ~ 13

Author(s):

H. YAMAGUCHI ◽

T. OSAKI ◽

H. TAGUCHI ◽

T. HANAWA ◽

T. YAMAMOTO ◽

...

Keyword(s):

Helicobacter Pylori ◽

Monoclonal Antibodies ◽

Heat Shock ◽

Heat Shock Protein ◽

Heat Shock Protein 60

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Comparison of the Serological Reactivity of Lipopolysaccharides from Japanese and Western Strains of Helicobacter pylori to Sera from H. pylori-Positive Humans

ISRN Microbiology ◽

10.5402/2012/162816 ◽

2012 ◽

Vol 2012 ◽

pp. 1-4 ◽

Cited By ~ 2

Author(s):

Ken-ichi Amano ◽

Shin-ichi Yokota ◽

Mario A. Monteiro

Keyword(s):

Helicobacter Pylori ◽

Monoclonal Antibodies ◽

Japanese Patients ◽

Lewis Antigens ◽

Lewis Antigen ◽

Serological Reactivity ◽

H Pylori ◽

Antigenic Epitopes

We compared the serological reactivity of lipopolysaccharides (LPS) isolated from Japanese and Western strains of Helicobacter pylori against anti-Lewis antigen monoclonal antibodies and H. pylori-positive Japanese sera. The two LPS from Western strains (26695 and O:2) did not react with any sera from Japanese patients, while all LPS from Japanese strains and the Sydney strain reacted with these sera. We propose that LPS of all Japanese smooth strains share either one of two epitopes, which are termed highly antigenic and weakly antigenic epitopes, present in the O-polysaccharide portion, and these epitopes are independent the Lewis antigens. The present findings indicated that the two Western strains lacked the two epitopes, which are shared by all Japanese strains.

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