Experimental Detection of Plesiomonas shigelloides Antigen in Feces by ELISA and Reversed Passive Latex Agglutination Test as a Model for Campylobacter

Author(s):  
S. Yamamoto ◽  
K. Tagata ◽  
Y. Isayama ◽  
M. Fukuyama
Author(s):  
Muhammad Shoaib ◽  
Kokab Furqan ◽  
Sajjad ur Rahman ◽  
Ahsan Naveed ◽  
Amjad Islam Aqib ◽  
...  

1989 ◽  
Vol 3 (1) ◽  
pp. 35-40 ◽  
Author(s):  
P. Morgan-Capner ◽  
P. Hudson ◽  
J.M. Best ◽  
A.J. Tilzey ◽  
S.A. Woodward ◽  
...  

1970 ◽  
Vol 56 (6) ◽  
pp. 1253 ◽  
Author(s):  
J. F. Williams ◽  
U. Prezioso

1970 ◽  
Vol 9 (4) ◽  
pp. 216-222 ◽  
Author(s):  
Md Abdus Salam ◽  
Dinesh Mondal ◽  
Mamun Kabir ◽  
Rashidul Haque

Background: A new unique latex agglutination test (KAtex) that detects a stable, nonprotein, disease specific parasite antigen in the freshly voided urine of patients suffering from active kala-azar has been introduced by Kalon Biological Ltd. UK. This is absolutely non-invasive method of diagnosis for visceral leishmaniasis and suitable for implementation as a rapid diagnostic tool at the point of care. Objective: Diagnostic potential of KAtex was evaluated among clinically suspected kala-azar patients in an endemic zone of Bangladesh. Methodology: KAtex was done using freshly voided urine according to the manufacturer’s instructions for sixty (60) clinically suspected patients of kala-azar admitted in Rajshahi Medical College Hospital (RMCH), Bangladesh and forty (40) healthy controls during December 2005 to June 2006. Leishmania nested Polymerase Chain Reaction (Ln-PCR) using peripheral blood buffy coat was performed for all study population (100) and Ln-PCR positive cases were considered as confirmed cases of kalaazar. Results: Out of 60 clinically suspected kala-azar patients, 56 were Ln-PCR positive and 53 of 56 Ln-PCR positive cases were KAtex positive (sensitivity, 94.64%; Mantel-Haenszel Chi sq. 79.66, p= 0.0000, confidence interval [CI], >95 to 100%). None of the healthy controls was found positive by Ln-PCR but 2 of 40 were KAtex positive (specificity, 95%; confidence interval [CI], >95 to 100%). The positive and negative predictive values of KAtex were noted as 98.10% and 92.85% respectively. Conclusion: This limited prospective study suggests that KAtex is an absolutely non-invasive urinebased antigen detection test with high sensitivity and specificity and may be useful for screening active kala-azar patients, particularly suitable for field use. Key words: Visceral leishmaniasis; Kala-azar; KAtex; Ln-PCR; sensitivity; specificity. DOI: 10.3329/bjms.v9i4.6688Bangladesh Journal of Medical Science Vol.09 No.4 July 2010 pp.216-222


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