Elicitation of Organized Pigmentation Patterns by a Chalcone Synthase Transgene

1993 ◽  
pp. 87-92 ◽  
Author(s):  
Richard A. Jorgensen
Keyword(s):  
Chalcone Synthase

Molecular Evolution of Chalcone Synthase Gene Superfamily in Plants

2015 ◽  
Vol 50 (1) ◽  
pp. 55
Author(s):  
Bao Ying ◽  
Guo Changfeng ◽  
Chen Shaohua ◽  
Liu Mei
Keyword(s):  
Molecular Evolution ◽  
Chalcone Synthase ◽  
Chalcone Synthase Gene

Genetic control of chalcone synthase activity in flowers of Antirrhinum majus

1982 ◽  
Vol 21 (9) ◽  
pp. 2231-2234 ◽  
Author(s):  
Regine Spribille ◽  
Gert Forkmann
Keyword(s):  
Genetic Control ◽  
Chalcone Synthase ◽  
Antirrhinum Majus

The nucleotide sequence of gene 1 of the soybean chalcone synthase multigene family

1991 ◽  
Vol 16 (4) ◽  
pp. 751-752 ◽  
Author(s):  
S. Akada ◽  
S. D. Kung ◽  
S. K. Dube
Keyword(s):  
Nucleotide Sequence ◽  
Multigene Family ◽  
Chalcone Synthase

scholarly journals Understanding Alstroemeria pallida Flower Colour: Links between Phenotype, Anthocyanins and Gene Expression

Plants ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 55
Author(s):  
Amanda Donoso ◽  
Constanza Rivas ◽  
Alan Zamorano ◽  
Álvaro Peña ◽  
Michael Handford ◽  
...  
Keyword(s):  
High Performance ◽  
Chalcone Synthase ◽  
Ornamental Plants ◽  
Flower Colour ◽  
Anthocyanin Content ◽  
Anthocyanidin Synthase ◽  
Visual Evaluation ◽  
Royal Horticultural Society ◽  
Polymerase Chain ◽  
Colour Chart

Flower colour is mainly due to the accumulation of flavonoids, carotenoids and betalains in the petals. Of these pigments, flavonoids are responsible for a wide variety of colours ranging from pale yellow (flavones, flavonols and flavanodiols) to blue-violet (anthocyanins). This character plays a crucial ecological role by attracting and guiding pollinators. Moreover, in the ornamental plants market, colour has been consistently identified as the main feature chosen by consumers when buying flowers. Considering the importance of this character, the aim of this study was to evaluate flower colour in the native Chilean geophyte Alstroemeria pallida, by using three different approaches. Firstly, the phenotype was assessed using both a colour chart and a colourimeter, obtaining CIELab parameters. Secondly, the anthocyanin content of the pigmented tepals was evaluated by high-performance liquid chromatography (HPLC), and finally, the expression of two key flavonoid genes, chalcone synthase (CHS) and anthocyanidin synthase (ANS) was analysed using real-time polymerase chain reaction (PCR). Visual evaluation of A. pallida flower colour identified 5 accessions, ranging from white (Royal Horticultural Society (RHS) N999D) to pink (RHS 68C). Moreover, this visual evaluation of the accessions correlated highly with the CIELab parameters obtained by colourimetry. An anthocyanidin corresponding to a putative 6-hydroxycyanidin was identified, which was least abundant in the white accession (RHS N999D). Although CHS was not expressed differentially between the accessions, the expression of ANS was significantly higher in the accession with pink flowers (RHS 68C). These results suggest a correlation between phenotype, anthocyanin content and ANS expression for determining flower colour of A. pallida, which could be of interest for further studies, especially those related to the breeding of this species with ornamental value.

Purification of Chalcone Synthase from Tulip Anthers and Comparison with the Synthase from Cosmos Petals

1981 ◽  
Vol 36 (1-2) ◽  
pp. 30-34 ◽  
Author(s):  
Rainer Sütfeld ◽  
Rolf Wiermann
Keyword(s):  
Chalcone Synthase ◽  
In Vitro Assay ◽  
Flavonoid Biosynthesis ◽  
Chalcone Isomerase ◽  
Gel Chromatography ◽  
Purification Procedure ◽  
Reaction Products ◽  
Complete Elimination ◽  
Vitro Assay

Abstract Chalcone synthase was isolated from both anthers of Tulipa cv. “Apeldoorn” and petals of Cosmos sulphureus Cav. After certain prepurification steps, the enzymes were further purified using gel chromatography on Sephadex G-200 followed by repeated hydroxylapatite absorption chromatography. Both the enzymes showed the same chromatographic properties. After gel chromatography as well as after the first hydroxylapatite fractionation, the reaction products appeared as flavanones. However, after the second hydroxylapatite step, production of chalcones was observed. Like the enzyme from tulip anthers, the synthase from Cosmos petals produced the correspondingly substituted chalcones when p-coumaroyl-CoA, caffeoyl-CoA and feruloyl-CoA, respectively, were used as substractes. In both the cases, the ratios of the different chalcones produced were found to be about the same. The appearance of chalcone synthesis in this in vitro assay is caused by the complete elimination of chalcone isomerase in the purification procedure. The importance of the isomerase for flavonoid biosynthesis, particularly in plant systems which are accumulating chalcones, is discussed.

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