Optimizing the Skeletal Muscle Biopsy

2014 ◽  
pp. 397-410
Author(s):  
Karen M. Weidenheim
Keyword(s):  
Skeletal Muscle ◽  
Muscle Biopsy ◽  
Skeletal Muscle Biopsy

Internalized capillaries in skeletal muscle biopsy

2015 ◽  
Vol 25 (1) ◽  
pp. 94-95
Author(s):  
Andreas Hawlik ◽  
Anette Wassner ◽  
Albert C. Ludolph ◽  
Jan Lewerenz ◽  
Angela Rosenbohm
Keyword(s):  
Skeletal Muscle ◽  
Muscle Biopsy ◽  
Skeletal Muscle Biopsy

Skeletal muscle biopsy

1982 ◽  
Vol 1 (3) ◽  
pp. 127-127 ◽  
Author(s):  
Victor J. Ojeda ◽  
Dominic V. Spagnolo ◽  
Keith Cole ◽  
Phillip F. Jacobsen
Keyword(s):  
Skeletal Muscle ◽  
Muscle Biopsy ◽  
Skeletal Muscle Biopsy

Enzymic Analysis of Human Skeletal Muscle Biopsy Samples

1981 ◽  
Vol 60 (3) ◽  
pp. 19P-19P
Author(s):  
F. Martin ◽  
J. Levi ◽  
G. Slavin ◽  
T. J. Peters
Keyword(s):  
Skeletal Muscle ◽  
Muscle Biopsy ◽  
Human Skeletal Muscle ◽  
Skeletal Muscle Biopsy

Role of repeat skeletal muscle biopsy: How useful is it?

2013 ◽  
Vol 47 (6) ◽  
pp. 835-839 ◽  
Author(s):  
Stephen A. Goutman ◽  
Richard A. Prayson
Keyword(s):  
Skeletal Muscle ◽  
Muscle Biopsy ◽  
Skeletal Muscle Biopsy

scholarly journals Optimization of the pre-analytical stage of material processing for histochemical examination of skeletal muscle biopsies in the diagnosis of neuromuscular diseases

2019 ◽  
Vol 9 (2) ◽  
pp. 21-29
Author(s):  
A. M. Sycheva ◽  
V. D. Nazarova ◽  
S. V. Lapin ◽  
M. G. Rybakova ◽  
D. I. Rudenko
Keyword(s):  
Skeletal Muscle ◽  
Muscle Biopsy ◽  
Morphological Changes ◽  
Clinical Manifestations ◽  
Neuromuscular Diseases ◽  
Optimal Method ◽  
Tissue Samples ◽  
Important Place ◽  
Skeletal Muscle Biopsy ◽  
Muscle Biopsies

Diagnosis of neuromuscular diseases is complicated by the variety of clinical manifestations and requires the use of additional methods, an important place among which is the pathomorphological study of skeletal muscle biopsy. Despite the fact that the procedure for taking a muscle biopsy is not technically difficult, to obtain informative material a multitude of conditions must be observed at the stages of pre-analytical processing of the obtained tissue samples. Violation of the technology of taking, storing and fixing the material contributes to the formation of artifacts that limit the possibilities for further analysis of the morphological changes in tissue biopsy. A comparison was made of the effectiveness of various methods for cryoprocessing of muscle tissue samples and the manufacture of histological specimens with a subsequent assessment of morphological changes. As a result, the main causes of artifacts were identified. The optimal method for processing muscle biopsy specimens is indicated, which makes it possible to prevent the appearance of artifacts as much as possible and to ensure the preservation of tissue for research.

scholarly journals Gene expression profiling in human skeletal muscle during recovery from eccentric exercise

2008 ◽  
Vol 294 (6) ◽  
pp. R1901-R1910 ◽  
Author(s):  
D. J. Mahoney ◽  
A. Safdar ◽  
G. Parise ◽  
S. Melov ◽  
Minghua Fu ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Real Time ◽  
Muscle Damage ◽  
Muscle Biopsy ◽  
Muscle Growth ◽  
Lipid Synthesis ◽  
Rt Pcr ◽  
Protein Z ◽  
Skeletal Muscle Biopsy ◽  
Exercise Induced

We used cDNA microarrays to screen for differentially expressed genes during recovery from exercise-induced muscle damage in humans. Male subjects ( n = 4) performed 300 maximal eccentric contractions, and skeletal muscle biopsy samples were analyzed at 3 h and 48 h after exercise. In total, 113 genes increased 3 h postexercise, and 34 decreased. At 48 h postexercise, 59 genes increased and 29 decreased. On the basis of these data, we chose 19 gene changes and conducted secondary analyses using real-time RT-PCR from muscle biopsy samples taken from 11 additional subjects who performed an identical bout of exercise. Real-time RT-PCR analyses confirmed that exercise-induced muscle damage led to a rapid (3 h) increase in sterol response element binding protein 2 ( SREBP-2), followed by a delayed (48 h) increase in the SREBP-2 gene targets Acyl CoA:cholesterol acyltransferase ( ACAT)-2 and insulin-induced gene 1 ( insig-1). The expression of the IL-1 receptor, a known regulator of SREBP-2, was also elevated after exercise. Taken together, these expression changes suggest a transcriptional program for increasing cholesterol and lipid synthesis and/or modification. Additionally, damaging exercise induced the expression of protein kinase H11, capping protein Z alpha ( capZα), and modulatory calcineurin-interacting protein 1 ( MCIP1), as well as cardiac ankryin repeat protein 1 ( CARP1), DNAJB2, c-myc, and junD, each of which are likely involved in skeletal muscle growth, remodeling, and stress management. In summary, using DNA microarrays and RT-PCR, we have identified novel genes that respond to skeletal muscle damage, which, given the known biological functions, are likely involved in recovery from and/or adaptation to damaging exercise.

Hindlimb Muscle Dissection, Permeabilization, and Respirometry v1

2021 ◽  
Author(s):  
Matthew D. Campbell ◽  
David J. Marcinek
Keyword(s):  
Skeletal Muscle ◽  
Muscle Biopsy ◽  
Mitochondrial Respiration ◽  
Gastrocnemius Muscle ◽  
Muscle Fibers ◽  
Mouse Muscle ◽  
Skeletal Muscle Biopsy ◽  
Cellular Environment ◽  
Permeabilized Muscle

The use of permeabilized muscle fibers (PMF) has emerged as a gold standard for assessing skeletal muscle mitochondrial function. PMF provide an intermediate approach between in vivo strategies and isolated mitochondria that allows the mitochondria to be maintained in close to their native morphology in the myofiber while allowing greater control of substrate and inhibitor concentrations. However, like mitochondrial isolation, the primary drawback to PMF is disruption of the cellular environment during the muscle biopsy and preparation. Despite all the benefits of permeabilized muscle fibers in evaluating mitochondrial respiration and dynamics one of the major drawbacks is increased variability introduced during a muscle biopsy as well as intrinsic variation that exists due to sex and age. This study was designed to evaluate how age, sex, and biopsy preparations affect mitochondrial respiration in extensor digitorum longus, soleus, and gastrocnemius muscle of mice. Here we detail a modified approach to skeletal muscle biopsy of the gastrocnemius muscle of mice focused on maintenance of intact fibers that results in greater overall respiration compared to cut fibers. The improved respiration of intact fibers is sex specific as are some of the changes in mitochondrial respiration with age. This study shows the need for standard practices when measuring mitochondrial respiration in permeabilized muscle and provides a protocol to control for variation introduced during a typical mouse muscle biopsy.

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