Enzymic Analysis of Human Skeletal Muscle Biopsy Samples

1981 ◽  
Vol 60 (3) ◽  
pp. 19P-19P
Author(s):  
F. Martin ◽  
J. Levi ◽  
G. Slavin ◽  
T. J. Peters
Keyword(s):  
Skeletal Muscle ◽  
Muscle Biopsy ◽  
Human Skeletal Muscle ◽  
Skeletal Muscle Biopsy

Intramuscular triacylglycerol utilization in human skeletal muscle during exercise: is there a controversy?

2002 ◽  
Vol 93 (4) ◽  
pp. 1185-1195 ◽  
Author(s):  
Matthew J. Watt ◽  
George J. F. Heigenhauser ◽  
Lawrence L. Spriet
Keyword(s):  
Skeletal Muscle ◽  
Muscle Biopsy ◽  
Human Skeletal Muscle ◽  
Moderate Intensity ◽  
Resonance Spectroscopy ◽  
Moderate Exercise ◽  
Moderate Intensity Exercise ◽  
Trained Subjects ◽  
Skeletal Muscle Biopsy ◽  
Isotope Tracer

Intramuscular triacylglyerols (IMTGs) represent a potentially important energy source for contracting human skeletal muscle. Although the majority of evidence from isotope tracer and 1H-magnetic resonance spectroscopy (MRS) studies demonstrate IMTG utilization during exercise, controversy regarding the importance of IMTG as a metabolic substrate persists. The controversy stems from studies that measure IMTG in skeletal muscle biopsy samples and report no significant net IMTG degradation during prolonged moderate-intensity (55–70% maximal O2 consumption) exercise lasting 90–120 min. Although postexercise decrements in IMTG levels are often reported from direct muscle measurements, the marked between-biopsy variability (∼23%) that has been reported with this technique in untrained subjects is larger than the expected decrease in IMTG content, effectively precluding significant findings. In contrast, recent data obtained in endurance-trained subjects demonstrated reduced variability between duplicate biopsies (∼12%), and significant changes in IMTG were detected after 120 min of moderate-intensity exercise. Therefore, it is our contention that the muscle biopsy, isotope tracer, and 1H-MRS techniques report significant and energetically important oxidation of free fatty acids derived from IMTGs during prolonged moderate exercise.

Characterization of Human Skeletal Muscle Biopsy Samples Using Shotgun Proteomics†

2009 ◽  
Vol 8 (7) ◽  
pp. 3265-3277 ◽  
Author(s):  
Kenneth C. Parker ◽  
Ronan J. Walsh ◽  
Mohammad Salajegheh ◽  
Anthony A. Amato ◽  
Bryan Krastins ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Muscle Biopsy ◽  
Human Skeletal Muscle ◽  
Shotgun Proteomics ◽  
Skeletal Muscle Biopsy

Effects of delayed freezing on content of phosphagens in human skeletal muscle biopsy samples

1986 ◽  
Vol 61 (3) ◽  
pp. 832-835 ◽  
Author(s):  
K. Soderlund ◽  
E. Hultman
Keyword(s):  
Skeletal Muscle ◽  
Muscle Biopsy ◽  
Human Skeletal Muscle ◽  
Lactate Concentration ◽  
Energy Utilization ◽  
Gas Mixture ◽  
Rapid Freezing ◽  
Atp Content ◽  
Fresh Tissue ◽  
Skeletal Muscle Biopsy

The concentrations of ATP, phosphocreatine (PCr), creatine, and lactate were determined in muscle biopsy samples frozen immediately or after a delay of 1–6 min. During the delay the samples were exposed to normal air or a gas mixture of 6.5% CO2-93.5% O2. The ATP content was unchanged, but PCr increased significantly from 72 mmol after rapid freezing to 85 mmol X kg dry muscle-1 during the 1st min in air. The lactate concentration increased (2.8 to 5.2 mmol X kg-1). If muscles were made anoxic by circulatory occlusion for 4–6 min before sampling, no increase in PCr was observed. Direct homogenization of fresh tissue in perchloric acid gave the same ATP, PCr, and lactate contents as frozen samples. It is concluded that the ATP and PCr contents in muscle are unaffected by freezing but that the biopsy procedure activates the energy utilization processes resulting in PCr decrease. It is suggested that the muscle PCr content after a 1-min delay in tissue freezing corresponds to the level in resting fresh muscle.

scholarly journals Human Skeletal Muscle Biopsy Procedures Using the Modified Bergström Technique

10.3791/51812 ◽  
2014 ◽  
Author(s):  
R. Andrew Shanely ◽  
Kevin A. Zwetsloot ◽  
N. Travis Triplett ◽  
Mary Pat Meaney ◽  
Gerard E. Farris ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Muscle Biopsy ◽  
Human Skeletal Muscle ◽  
Skeletal Muscle Biopsy

Investigation of mitochondrial metabolism in small human skeletal muscle biopsy specimens. Improvement of preparation procedure

1985 ◽  
Vol 145 (1) ◽  
pp. 89-99 ◽  
Author(s):  
Johan C. Fischer ◽  
Wim Ruitenbeek ◽  
Ad M. Stadhouders ◽  
J.M.Frans Trijbels ◽  
Rob C.A. Sengers ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Muscle Biopsy ◽  
Human Skeletal Muscle ◽  
Mitochondrial Metabolism ◽  
Preparation Procedure ◽  
Skeletal Muscle Biopsy ◽  
Biopsy Specimens

Internalized capillaries in skeletal muscle biopsy

2015 ◽  
Vol 25 (1) ◽  
pp. 94-95
Author(s):  
Andreas Hawlik ◽  
Anette Wassner ◽  
Albert C. Ludolph ◽  
Jan Lewerenz ◽  
Angela Rosenbohm
Keyword(s):  
Skeletal Muscle ◽  
Muscle Biopsy ◽  
Skeletal Muscle Biopsy
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