scholarly journals Optimization of the pre-analytical stage of material processing for histochemical examination of skeletal muscle biopsies in the diagnosis of neuromuscular diseases

2019 ◽  
Vol 9 (2) ◽  
pp. 21-29
Author(s):  
A. M. Sycheva ◽  
V. D. Nazarova ◽  
S. V. Lapin ◽  
M. G. Rybakova ◽  
D. I. Rudenko
Keyword(s):  
Skeletal Muscle ◽  
Muscle Biopsy ◽  
Morphological Changes ◽  
Clinical Manifestations ◽  
Neuromuscular Diseases ◽  
Optimal Method ◽  
Tissue Samples ◽  
Important Place ◽  
Skeletal Muscle Biopsy ◽  
Muscle Biopsies

Diagnosis of neuromuscular diseases is complicated by the variety of clinical manifestations and requires the use of additional methods, an important place among which is the pathomorphological study of skeletal muscle biopsy. Despite the fact that the procedure for taking a muscle biopsy is not technically difficult, to obtain informative material a multitude of conditions must be observed at the stages of pre-analytical processing of the obtained tissue samples. Violation of the technology of taking, storing and fixing the material contributes to the formation of artifacts that limit the possibilities for further analysis of the morphological changes in tissue biopsy. A comparison was made of the effectiveness of various methods for cryoprocessing of muscle tissue samples and the manufacture of histological specimens with a subsequent assessment of morphological changes. As a result, the main causes of artifacts were identified. The optimal method for processing muscle biopsy specimens is indicated, which makes it possible to prevent the appearance of artifacts as much as possible and to ensure the preservation of tissue for research.

Internalized capillaries in skeletal muscle biopsy

2015 ◽  
Vol 25 (1) ◽  
pp. 94-95
Author(s):  
Andreas Hawlik ◽  
Anette Wassner ◽  
Albert C. Ludolph ◽  
Jan Lewerenz ◽  
Angela Rosenbohm
Keyword(s):  
Skeletal Muscle ◽  
Muscle Biopsy ◽  
Skeletal Muscle Biopsy

scholarly journals When should MERRF (myoclonus epilepsy associated with ragged-red fibers) be the diagnosis?

2014 ◽  
Vol 72 (10) ◽  
pp. 803-811 ◽  
Author(s):  
Paulo José Lorenzoni ◽  
Rosana Herminia Scola ◽  
Cláudia Suemi Kamoi Kay ◽  
Carlos Eduardo S. Silvado ◽  
Lineu Cesar Werneck
Keyword(s):  
Muscle Biopsy ◽  
Morphological Changes ◽  
Mitochondrial Disorder ◽  
Point Mutations ◽  
Clinical Manifestations ◽  
Strong Reaction ◽  
Brain Images ◽  
Ragged Red Fibers ◽  
Myoclonus Epilepsy ◽  
Generalized Epilepsy

Myoclonic epilepsy associated with ragged red fibers (MERRF) is a rare mitochondrial disorder. Diagnostic criteria for MERRF include typical manifestations of the disease: myoclonus, generalized epilepsy, cerebellar ataxia and ragged red fibers (RRF) on muscle biopsy. Clinical features of MERRF are not necessarily uniform in the early stages of the disease, and correlations between clinical manifestations and physiopathology have not been fully elucidated. It is estimated that point mutations in the tRNALys gene of the DNAmt, mainly A8344G, are responsible for almost 90% of MERRF cases. Morphological changes seen upon muscle biopsy in MERRF include a substantive proportion of RRF, muscle fibers showing a deficient activity of cytochrome c oxidase (COX) and the presence of vessels with a strong reaction for succinate dehydrogenase and COX deficiency. In this review, we discuss mainly clinical and laboratory manifestations, brain images, electrophysiological patterns, histology and molecular findings as well as some differential diagnoses and treatments.

Skeletal muscle biopsy

1982 ◽  
Vol 1 (3) ◽  
pp. 127-127 ◽  
Author(s):  
Victor J. Ojeda ◽  
Dominic V. Spagnolo ◽  
Keith Cole ◽  
Phillip F. Jacobsen
Keyword(s):  
Skeletal Muscle ◽  
Muscle Biopsy ◽  
Skeletal Muscle Biopsy

Comparison of Erythrocyte and Skeletal Muscle Creatine Accumulation Following Creatine Loading

2005 ◽  
Vol 15 (1) ◽  
pp. 84-93 ◽  
Author(s):  
David B. Preen ◽  
Brian T. Dawson ◽  
Carmel Goodman ◽  
John Beilby ◽  
Simon Ching
Keyword(s):  
Skeletal Muscle ◽  
Muscle Biopsy ◽  
Vastus Lateralis ◽  
Venous Blood ◽  
Strong Relationship ◽  
Reliable Measure ◽  
Tissue Concentrations ◽  
Muscle Creatine ◽  
The Relationship ◽  
Muscle Biopsies

This study attempted to determine the relationship between creatine (Cr) accumulation in human skeletal muscle and erythrocytes following Cr supplementation. If a strong relationship exists, a blood test might provide a practical, less invasive alternative than muscle biopsy for evaluating cellular Cr accumulation. Eighteen active, but not well-trained males were supplemented with Cr (4 × 5g/d) for 5 d. Muscle biopsies (vastus lateralis) were obtained pre- and post-loading and analyzed for Cr, phosphocreatine (PCr), and total Cr (TCr) content. Venous blood was also drawn at these times to determine erythrocyte Cr concentrations. Muscle Cr, PCr, and TCr concentrations were elevated (P < 0.05) by 39.8%, 7.5%, and 20.1% respectively following supplementation. Erythrocyte Cr concentrations were also elevated (P < 0.01) following the loading period, although to a greater relative degree than tissue concentrations (129.6%). Pre- and post-loading erythrocyte Cr concentrations were poorly and nonsignificantly correlated with that observed in skeletal muscle. Further, loading-mediated increases in erythrocyte Cr concentrations were poorly correlated with elevations in muscle Cr (r = 0.07), PCr (r = 0.06) or TCr (r = 0.04) concentrations. Erythrocyte Cr concentrations can be augmented by 5 d of Cr supplementation, however, this elevation does not reflect that observed in skeletal muscle obtained by muscle biopsy. Consequently, erythrocyte response to Cr loading is not a reliable measure of skeletal muscle Cr/TCr accumulation.

Enzymic Analysis of Human Skeletal Muscle Biopsy Samples

1981 ◽  
Vol 60 (3) ◽  
pp. 19P-19P
Author(s):  
F. Martin ◽  
J. Levi ◽  
G. Slavin ◽  
T. J. Peters
Keyword(s):  
Skeletal Muscle ◽  
Muscle Biopsy ◽  
Human Skeletal Muscle ◽  
Skeletal Muscle Biopsy

Role of repeat skeletal muscle biopsy: How useful is it?

2013 ◽  
Vol 47 (6) ◽  
pp. 835-839 ◽  
Author(s):  
Stephen A. Goutman ◽  
Richard A. Prayson
Keyword(s):  
Skeletal Muscle ◽  
Muscle Biopsy ◽  
Skeletal Muscle Biopsy

scholarly journals Gene expression profiling in human skeletal muscle during recovery from eccentric exercise

2008 ◽  
Vol 294 (6) ◽  
pp. R1901-R1910 ◽  
Author(s):  
D. J. Mahoney ◽  
A. Safdar ◽  
G. Parise ◽  
S. Melov ◽  
Minghua Fu ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Real Time ◽  
Muscle Damage ◽  
Muscle Biopsy ◽  
Muscle Growth ◽  
Lipid Synthesis ◽  
Rt Pcr ◽  
Protein Z ◽  
Skeletal Muscle Biopsy ◽  
Exercise Induced

We used cDNA microarrays to screen for differentially expressed genes during recovery from exercise-induced muscle damage in humans. Male subjects ( n = 4) performed 300 maximal eccentric contractions, and skeletal muscle biopsy samples were analyzed at 3 h and 48 h after exercise. In total, 113 genes increased 3 h postexercise, and 34 decreased. At 48 h postexercise, 59 genes increased and 29 decreased. On the basis of these data, we chose 19 gene changes and conducted secondary analyses using real-time RT-PCR from muscle biopsy samples taken from 11 additional subjects who performed an identical bout of exercise. Real-time RT-PCR analyses confirmed that exercise-induced muscle damage led to a rapid (3 h) increase in sterol response element binding protein 2 ( SREBP-2), followed by a delayed (48 h) increase in the SREBP-2 gene targets Acyl CoA:cholesterol acyltransferase ( ACAT)-2 and insulin-induced gene 1 ( insig-1). The expression of the IL-1 receptor, a known regulator of SREBP-2, was also elevated after exercise. Taken together, these expression changes suggest a transcriptional program for increasing cholesterol and lipid synthesis and/or modification. Additionally, damaging exercise induced the expression of protein kinase H11, capping protein Z alpha ( capZα), and modulatory calcineurin-interacting protein 1 ( MCIP1), as well as cardiac ankryin repeat protein 1 ( CARP1), DNAJB2, c-myc, and junD, each of which are likely involved in skeletal muscle growth, remodeling, and stress management. In summary, using DNA microarrays and RT-PCR, we have identified novel genes that respond to skeletal muscle damage, which, given the known biological functions, are likely involved in recovery from and/or adaptation to damaging exercise.

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