Metabolic Flux Analysis for Escherichia coli by Flux Balance Analysis

2014 ◽  
pp. 237-260 ◽  
Author(s):  
Yu Matsuoka ◽  
Kazuyuki Shimizu
Keyword(s):  
Escherichia Coli ◽  
Flux Balance Analysis ◽  
Metabolic Flux Analysis ◽  
Metabolic Flux ◽  
Flux Analysis ◽  
Flux Balance ◽  
Balance Analysis

Synergy between 13C-metabolic flux analysis and flux balance analysis for understanding metabolic adaption to anaerobiosis in E. coli

2011 ◽  
Vol 13 (1) ◽  
pp. 38-48 ◽  
Author(s):  
Xuewen Chen ◽  
Ana P. Alonso ◽  
Doug K. Allen ◽  
Jennifer L. Reed ◽  
Yair Shachar-Hill
Keyword(s):  
Flux Balance Analysis ◽  
Metabolic Flux Analysis ◽  
Metabolic Flux ◽  
Flux Analysis ◽  
E Coli ◽  
13C Metabolic Flux Analysis ◽  
Flux Balance ◽  
Balance Analysis

scholarly journals Physicochemical and metabolic constraints for thermodynamics-based stoichiometric modelling under mesophilic growth conditions

2020 ◽  
Author(s):  
Claudio Tomi-Andrino ◽  
Rupert Norman ◽  
Thomas Millat ◽  
Philippe Soucaille ◽  
Klaus Winzer ◽  
...  
Keyword(s):  
Experimental Data ◽  
Metabolic Flux Analysis ◽  
In Silico ◽  
Physicochemical Parameters ◽  
Metabolic Flux ◽  
Living Systems ◽  
Flux Analysis ◽  
Flux Balance ◽  
Metabolic Fluxes ◽  
Balance Analysis

AbstractMetabolic engineering in the post-genomic era is characterised by the development of new methods for metabolomics and fluxomics, supported by the integration of genetic engineering tools and mathematical modelling. Particularly, constraint-based stoichiometric models have been widely studied: (i) flux balance analysis (FBA) (in silico), and (ii) metabolic flux analysis (MFA) (in vivo). Recent studies have enabled the incorporation of thermodynamics and metabolomics data to improve the predictive capabilities of these approaches. However, an in-depth comparison and evaluation of these methods is lacking. This study presents a thorough analysis of two different in silico methods tested against experimental data (metabolomics and 13C-MFA) for the mesophile Escherichia coli. In particular, a modified version of the recently published matTFA toolbox was created, providing a broader range of physicochemical parameters. Validating against experimental data allowed the determination of the best physicochemical parameters to perform the TFA (Thermodynamics-based Flux Analysis). An analysis of flux pattern changes in the central carbon metabolism between 13C-MFA and TFA highlighted the limited capabilities of both approaches for elucidating the anaplerotic fluxes. In addition, a method based on centrality measures was suggested to identify important metabolites that (if quantified) would allow to further constrain the TFA. Finally, this study emphasised the need for standardisation in the fluxomics community: novel approaches are frequently released but a thorough comparison with currently accepted methods is not always performed.Author summaryBiotechnology has benefitted from the development of high throughput methods characterising living systems at different levels (e.g. concerning genes or proteins), allowing the industrial production of chemical commodities. Recently, focus has been placed on determining reaction rates (or metabolic fluxes) in the metabolic network of certain microorganisms, in order to identify bottlenecks hindering their exploitation. Two main approaches are commonly used, termed metabolic flux analysis (MFA) and flux balance analysis (FBA), based on measuring and estimating fluxes, respectively. While the influence of thermodynamics in living systems was accepted several decades ago, its application to study biochemical networks has only recently been enabled. In this sense, a multitude of different approaches constraining well-established modelling methods with thermodynamics has been suggested. However, physicochemical parameters are generally not properly adjusted to the experimental conditions, which might affect their predictive capabilities. In this study, we have explored the reliability of currently available tools by investigating the impact of varying said parameters in the simulation of metabolic fluxes and metabolite concentration values. Additionally, our in-depth analysis allowed us to highlight limitations and potential solutions that should be considered in future studies.

scholarly journals How to Tackle Underdeterminacy in Metabolic Flux Analysis? A Tutorial and Critical Review

Processes ◽  
2021 ◽  
Vol 9 (9) ◽  
pp. 1577
Author(s):  
Philippe Bogaerts ◽  
Alain Vande Vande Wouwer
Keyword(s):  
Metabolic Flux Analysis ◽  
Metabolic Flux ◽  
Convex Polytope ◽  
Flux Analysis ◽  
Linear Algebraic System ◽  
Balance Equations ◽  
Flux Variability Analysis ◽  
Variability Analysis ◽  
Flux Balance ◽  
Balance Analysis

Metabolic flux analysis is often (not to say almost always) faced with system underdeterminacy. Indeed, the linear algebraic system formed by the steady-state mass balance equations around the intracellular metabolites and the equality constraints related to the measurements of extracellular fluxes do not define a unique solution for the distribution of intracellular fluxes, but instead a set of solutions belonging to a convex polytope. Various methods have been proposed to tackle this underdeterminacy, including flux pathway analysis, flux balance analysis, flux variability analysis and sampling. These approaches are reviewed in this article and a toy example supports the discussion with illustrative numerical results.

scholarly journals Investigation of useful carbon tracers for 13 C-metabolic flux analysis of Escherichia coli by considering five experimentally determined flux distributions

2016 ◽  
Vol 3 ◽  
pp. 187-195 ◽  
Author(s):  
Kousuke Maeda ◽  
Nobuyuki Okahashi ◽  
Yoshihiro Toya ◽  
Fumio Matsuda ◽  
Hiroshi Shimizu
Keyword(s):  
Escherichia Coli ◽  
Metabolic Flux Analysis ◽  
Metabolic Flux ◽  
Flux Analysis
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