Three-Dimensional Spheroid Cell Culture Model for Target Identification Utilizing High-Throughput RNAi Screens

2016 ◽  
pp. 121-135 ◽  
Author(s):  
LaKesla R. Iles ◽  
Geoffrey A. Bartholomeusz
Keyword(s):  
Cell Culture ◽  
High Throughput ◽  
Target Identification ◽  
Three Dimensional ◽  
Cell Culture Model ◽  
Culture Model ◽  
Spheroid Cell Culture

Cell-free DNA in a three-dimensional spheroid cell culture model: A preliminary study

2017 ◽  
Vol 89 ◽  
pp. 182-192 ◽  
Author(s):  
Janine Aucamp ◽  
Carlemi Calitz ◽  
Abel J. Bronkhorst ◽  
Krzysztof Wrzesinski ◽  
Sias Hamman ◽  
...  
Keyword(s):  
Cell Culture ◽  
Three Dimensional ◽  
Cell Culture Model ◽  
Cell Free Dna ◽  
Free Dna ◽  
Culture Model ◽  
Preliminary Study ◽  
Spheroid Cell Culture

S1792 Effects of Ethanol and Acetaldehyde on Epithelial Integrity in a Three Dimensional (3D) Epithelial Cell Culture Model

2010 ◽  
Vol 138 (5) ◽  
pp. S-275
Author(s):  
Elhaseen Elamin ◽  
Daisy Jonkers ◽  
Freddy Troost ◽  
Kati Juuti-Uusitalo ◽  
Sven C. van IJzendoorn ◽  
...  
Keyword(s):  
Cell Culture ◽  
Epithelial Cell ◽  
Three Dimensional ◽  
Cell Culture Model ◽  
Epithelial Cell Culture ◽  
Epithelial Integrity ◽  
Culture Model

scholarly journals Effects of Ethanol and Acetaldehyde on Tight Junction Integrity: In Vitro Study in a Three Dimensional Intestinal Epithelial Cell Culture Model

PLoS ONE ◽  
2012 ◽  
Vol 7 (4) ◽  
pp. e35008 ◽  
Author(s):  
Elhaseen Elamin ◽  
Daisy Jonkers ◽  
Kati Juuti-Uusitalo ◽  
Sven van IJzendoorn ◽  
Freddy Troost ◽  
...  
Keyword(s):  
Cell Culture ◽  
Epithelial Cell ◽  
Intestinal Epithelial Cell ◽  
Three Dimensional ◽  
In Vitro Study ◽  
Intestinal Epithelial ◽  
Cell Culture Model ◽  
Epithelial Cell Culture ◽  
Culture Model

scholarly journals The Late-Stage Protective Effect of Mito-TEMPO against Acetaminophen-Induced Hepatotoxicity in Mouse and Three-Dimensional Cell Culture Models

Antioxidants ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 965
Author(s):  
Mohammad Abdullah-Al-Shoeb ◽  
Kenta Sasaki ◽  
Saori Kikutani ◽  
Nanami Namba ◽  
Keiichi Ueno ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cell Culture ◽  
Liver Injury ◽  
Protective Effect ◽  
Acute Liver Injury ◽  
Three Dimensional ◽  
3D Cell Culture ◽  
Cell Culture Model ◽  
Culture Model ◽  
Apap Hepatotoxicity

An overdose of acetaminophen (APAP), the most common cause of acute liver injury, induces oxidative stress that subsequently causes mitochondrial impairment and hepatic necroptosis. N-acetyl-L-cysteine (NAC), the only recognized drug against APAP hepatotoxicity, is less effective the later it is administered. This study evaluated the protective effect of mitochondria-specific Mito-TEMPO (Mito-T) on APAP-induced acute liver injury in C57BL/6J male mice, and a three dimensional (3D)-cell culture model containing the human hepatoblastoma cell line HepG2. The administration of Mito-T (20 mg/kg, i.p.) 1 h after APAP (400 mg/kg, i.p.) injection markedly attenuated the APAP-induced elevated serum transaminase activity and hepatic necrosis. However, Mito-T treatment did not affect key factors in the development of APAP liver injury including the activation of c-jun N-terminal kinases (JNK), and expression of the transcription factor C/EBP homologous protein (CHOP) in the liver. However, Mito-T significantly reduced the APAP-induced increase in the hepatic oxidative stress marker, nitrotyrosine, and DNA fragmentation. Mito-T markedly attenuated cytotoxicity induced by APAP in the HepG2 3D-cell culture model. Moreover, liver regeneration after APAP hepatotoxicity was not affected by Mito-T, demonstrated by no changes in proliferating cell nuclear antigen formation. Therefore, Mito-T was hepatoprotective at the late-stage of APAP overdose in mice.

A Three-dimensional Cell Culture Model for Bovine Endometrium: Regeneration of a Multicellular Spheroid Using Ascorbate

Placenta ◽  
2003 ◽  
Vol 24 (2-3) ◽  
pp. 258-269 ◽  
Author(s):  
N Yamauchi ◽  
O Yamada ◽  
T Takahashi ◽  
K Imai ◽  
T Sato ◽  
...  
Keyword(s):  
Cell Culture ◽  
Three Dimensional ◽  
Multicellular Spheroid ◽  
Cell Culture Model ◽  
Culture Model ◽  
Dimensional Cell

Cytocompatibility of Biodentine using a three-dimensional cell culture model

2015 ◽  
Vol 49 (6) ◽  
pp. 574-580 ◽  
Author(s):  
E. J. N. L. Silva ◽  
P. M. Senna ◽  
G. De-Deus ◽  
A. A. Zaia
Keyword(s):  
Cell Culture ◽  
Three Dimensional ◽  
Cell Culture Model ◽  
Culture Model ◽  
Dimensional Cell
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