Wheat Genetic Transformation Using Mature Embryos as Explants

2017 ◽  
pp. 153-167 ◽  
Author(s):  
Harsh Chauhan ◽  
Paramjit Khurana
Keyword(s):  
Genetic Transformation ◽  
Mature Embryos

scholarly journals Optimization of Mature Embryo-Based Tissue Culture and Agrobacterium-Mediated Transformation in Model Grass Brachypodium distachyon

2019 ◽  
Vol 20 (21) ◽  
pp. 5448 ◽  
Author(s):  
Yu ◽  
Wang ◽  
Miao ◽  
Xi ◽  
Wang ◽  
...  
Keyword(s):  
Genetic Transformation ◽  
Time Window ◽  
Brachypodium Distachyon ◽  
Immature Embryo ◽  
Mature Embryo ◽  
Mature Embryos ◽  
Root Regeneration ◽  
Development And Utilization ◽  
New Methodologies ◽  
Model Grass

Agrobacterium-mediated genetic transformation is well established in the model grass Brachypodium distachyon. However, most protocols employ immature embryos because of their better regenerative capacity. A major problem associated with the immature embryo system is that they are available only during a limited time window of growing plants. In this study, we have developed an optimized Agrobacterium-mediated genetic transformation protocol that utilizes mature embryos. We have adopted seed shearing and photoautotrophic rooting (PR) in callus induction and root regeneration, respectively, with evident significant improvement in these aspects. We have also revealed that the newly developed chemical inducer Fipexide (FPX) had the ability to induce callus, shoots, and roots. By comparison, we have demonstrated that FPX shows higher efficiency in shoot generation than other frequently used chemicals in our mature embryo-based system. In addition, we demonstrated that the age of embryogenetic callus severely affects the transformation efficiency (TE), with the seven-week-old embryogenetic callus having the highest TE reaching 52.6%, which is comparable with that in immature embryo transformation. The new methodologies reported here will advance the development and utilization of Brachypodium as a new model system for grass genomics.

scholarly journals Optimization of Agrobacterium tumefaciens mediated genetic transformation protocol for aromatic ric

1970 ◽  
Vol 7 (2) ◽  
pp. 235-240
Author(s):  
MR Hossain ◽  
L Hassan ◽  
AK Patwary ◽  
MI Ferdous
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Genetic Transformation ◽  
Aromatic Rice ◽  
Rice Varieties ◽  
Transformation Protocol ◽  
Mature Embryos ◽  
Gus Reporter ◽  
Established Plant ◽  
Hygromycin Resistance Gene ◽  
Almost All

A rapid, efficient and reproducible genetic transformation protocol was optimized for four aromatic rice varieties by using the established plant regeneration protocol. Mature embryos were inoculated with Agrobacterium tumefaciens strain EHA105 carrying a binary vector pIG121-Hm with GUS (reporter gene) and hpt (hygromycin resistance) gene and the transformation experiment was performed by optimizing two important parameters viz. infection times and co-cultivation periods. The highest response to GUS assay was showed by Kalizira (70% GUS positive) followed by Pusa Basmati 1 (66.67%) when the explants were inoculated for 25 minutes and co-cultivated for three days. Twenty five minutes infection time (9.44%) and three day co- cultivation period (8.06%) were found effective for percentage of transgenic shoot regeneration. The highest percentage of putative transgenic shoots was regenerated by variety Kalizira (33.33%) followed by Pusa Basmati 1 (20.0%) and Radhunipagol (13.33%). Among the varieties, Kalizira produced the highest percentage (60%) of rooted shoots. Kalizira also showed the highest survival rate in growth chamber (75%) and in field condition (60%). The performance of Tulsimala was poor for almost all the cases. Keywords: Agrobacterium; GUS; hpt gene; Transformation and Aromatic rice DOI: 10.3329/jbau.v7i2.4724 J. Bangladesh Agril. Univ. 7(2): 235-240, 2009

Genetic transformation of mature embryos of bread (T. aestivum) and pasta (T. durum) wheat genotypes

GM Crops ◽  
2010 ◽  
Vol 1 (2) ◽  
pp. 30-36 ◽  
Author(s):  
Reda E.A. Moghaieb ◽  
Nagwa I. El-Arabi ◽  
Osama A. Momtaz ◽  
Sawsan S. Youssef ◽  
Mohamed H. Soliman
Keyword(s):  
Genetic Transformation ◽  
Durum Wheat ◽  
Wheat Genotypes ◽  
Mature Embryos

Stable genetic transformation of Arabidopsis thaliana by Agrobacterium inoculation in planta

1994 ◽  
Vol 5 (4) ◽  
pp. 551-558 ◽  
Author(s):  
Seok So Chang ◽  
Soon Ki Park ◽  
Byung Chul Kim ◽  
Bong Joong Kang ◽  
Dal Ung Kim ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Genetic Transformation ◽  
In Planta

Genetic transformation of Ginkgo biloba by Agrobacterium tumefaciens

2000 ◽  
Vol 108 (4) ◽  
pp. 413-419
Author(s):  
Patricia Dupré ◽  
Jerôme Lacoux ◽  
Godfrey Neutelings ◽  
Dominique Mattar-Laurain ◽  
Marc-André Fliniaux ◽  
...  
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Genetic Transformation ◽  
Ginkgo Biloba

Construction of expression vectors of the melon resistance gene Fom-2 and genetic transformation

2018 ◽  
Vol 51 (1) ◽  
Author(s):  
Xiaomei Li ◽  
Liu Liu ◽  
Xiangyu Liu ◽  
Yan Hou ◽  
Bingyin Xu ◽  
...  
Keyword(s):  
Genetic Transformation ◽  
Resistance Gene ◽  
Expression Vectors

RNA Interference Vector Construction, Genetic Transformation and Function Analysis of Rice Transcription FactorOsMYB14

2013 ◽  
Vol 19 (6) ◽  
pp. 960
Author(s):  
Yanwen MIAO ◽  
Rong LI ◽  
Fangjie XIONG ◽  
Zhengming ZENG ◽  
Xiangli NIU ◽  
...  
Keyword(s):  
Rna Interference ◽  
Genetic Transformation ◽  
Function Analysis ◽  
Vector Construction ◽  
And Function

Construction and Genetic Transformation of TomatoARF4RNA Interference Expression Vector with Fruit Specific Promoter

2012 ◽  
Vol 18 (2) ◽  
pp. 206
Author(s):  
Yuanyuan FENG ◽  
Yingnan LI ◽  
Pei HOU ◽  
Jingrong ZHANG ◽  
Songhu WANG ◽  
...  
Keyword(s):  
Genetic Transformation ◽  
Expression Vector

Mitotic Activity Dynamics in Recalcitrant Seeds Acer sachharinum during Maturation and Germination

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 479c-479
Author(s):  
L. Kozeko ◽  
V. Troyan ◽  
L. Musatenko
Keyword(s):  
Cell Division ◽  
Mitotic Activity ◽  
Quiescent State ◽  
Activity Levels ◽  
Recalcitrant Seeds ◽  
Mature Embryos ◽  
Before And After ◽  
Orthodox Seeds ◽  
Mature Seeds ◽  
Activity Dynamics

In orthodox seeds the cell division within the embryo meristems arrests during maturation at embryo moisture content (MC) 65% to 47%, and the maturation completion and transition of seeds to quiescent state occurs at MC about 10%. The arrest of cycling happens asynchronously in different meristematic tissues during desiccation: first in shoot and then in root. The aim of this work was to define a mitotic activity dynamics in recalcitrant seeds with the high MC at maturation end and the absence of quiescent state characteristic of it. The object was seeds of Acer saccharinum, using widely for planting of greenery in Kiev city. The mitotic activity was determined in 0.5 mm of the embryo root pole (RP) and 0.5 mm of the shoot pole with embryo leaves (SP). The A. sachharinum seeds completed them maturation at MC 53% (FW basis). During maturation the mitotic index (MI) in RP decreased from 3.2% in immature seeds (at embryos MC 80%) to 0 in mature seeds and in SP–from 5.4% to 3.3%, respectively. Cell division in SP arrested by dehydration of mature embryos to MC 46% by PEG 6000 (30%). The seeds lost viability by desiccation to MC 34%. The mature seeds were able to germinate immediately after abscission. During seed germination the cell division reactived in RP and increased in SP already before root protrusion. In plantlets 10–15 mm long the MI increased to 8% in RP and 12% in SP. Thus, the strategy of immediate germination of recalcitrant A. sachharinum seeds includes a preservation of cell division in SP of mature embryos, in contrast with orthodox seeds, and high mitotic activity levels in meristems of germinating embryos before and after root protrusion.

Genetic transformation of commercial breeding lines of alfalfa (Medicago sativa)

1995 ◽  
Vol 42 (2) ◽  
pp. 129-140 ◽  
Author(s):  
R�jean Desgagn�s ◽  
Serge Laberge ◽  
Guy Allard ◽  
Habib Khoudi ◽  
Yves Castonguay ◽  
...  
Keyword(s):  
Genetic Transformation ◽  
Medicago Sativa ◽  
Breeding Lines ◽  
Commercial Breeding
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