scholarly journals Optimization of Mature Embryo-Based Tissue Culture and Agrobacterium-Mediated Transformation in Model Grass Brachypodium distachyon

2019 ◽  
Vol 20 (21) ◽  
pp. 5448 ◽  
Author(s):  
Yu ◽  
Wang ◽  
Miao ◽  
Xi ◽  
Wang ◽  
...  
Keyword(s):  
Genetic Transformation ◽  
Time Window ◽  
Brachypodium Distachyon ◽  
Immature Embryo ◽  
Mature Embryo ◽  
Mature Embryos ◽  
Root Regeneration ◽  
Development And Utilization ◽  
New Methodologies ◽  
Model Grass

Agrobacterium-mediated genetic transformation is well established in the model grass Brachypodium distachyon. However, most protocols employ immature embryos because of their better regenerative capacity. A major problem associated with the immature embryo system is that they are available only during a limited time window of growing plants. In this study, we have developed an optimized Agrobacterium-mediated genetic transformation protocol that utilizes mature embryos. We have adopted seed shearing and photoautotrophic rooting (PR) in callus induction and root regeneration, respectively, with evident significant improvement in these aspects. We have also revealed that the newly developed chemical inducer Fipexide (FPX) had the ability to induce callus, shoots, and roots. By comparison, we have demonstrated that FPX shows higher efficiency in shoot generation than other frequently used chemicals in our mature embryo-based system. In addition, we demonstrated that the age of embryogenetic callus severely affects the transformation efficiency (TE), with the seven-week-old embryogenetic callus having the highest TE reaching 52.6%, which is comparable with that in immature embryo transformation. The new methodologies reported here will advance the development and utilization of Brachypodium as a new model system for grass genomics.

Regeneration of immature and mature embryos from diverse sets of wheat genotypes using media containing different auxins

2012 ◽  
Vol 60 (2) ◽  
pp. 97-108 ◽  
Author(s):  
R. Murín ◽  
K. Mészáros ◽  
P. Nemeček ◽  
R. Kuna ◽  
J. Faragó
Keyword(s):  
Plant Regeneration ◽  
Immature Embryo ◽  
Shoot Formation ◽  
Mature Embryo ◽  
Regeneration Ability ◽  
Wheat Embryo ◽  
Mature Embryos ◽  
Embryo Cultures ◽  
2,4 Dichlorophenoxyacetic Acid

The effect of explant type (immature vs. mature embryos) and two auxin types (2,4-dichlorophenoxyacetic acid vs. Dicamba) on the callogenesis and plant regeneration ability of 26 wheat cultivars was studied. In general, the callus induction, plant regeneration and shoot formation frequencies were higher in mature embryo-derived cultures as compared to immature ones on media originally developed for mature wheat embryo cultures. In both culture types, the auxin Dicamba was found to be more efficient, especially when mature embryos were cultured. The separation of means using Duncan’s multiple range test revealed the best in vitro response, in terms of the frequency of callus regeneration, in the cultivar Astella for both immature and mature embryo cultures. This cultivar gave very promising results, suggesting that it could be used in the future for further tissue culture investigations and as a donor material for genetic transformation experiments in wheat. Correlation analyses revealed significant similarities between the evaluated parameters within each group (immature and mature embryo-derived cultures). However, there were no significant correlations between these two groups for most of the parameters. This suggests that the mechanism of plant regeneration in the two in vitro regeneration systems (mature vs. immature embryo culture) may be different enough to hamper the development of an optimal plant regeneration protocol for use in both systems.

scholarly journals Grain dormancy and light quality effects on germination in the model grass Brachypodium distachyon

2011 ◽  
Vol 193 (2) ◽  
pp. 376-386 ◽  
Author(s):  
José M. Barrero ◽  
John V. Jacobsen ◽  
Mark J. Talbot ◽  
Rosemary G. White ◽  
Stephen M. Swain ◽  
...  
Keyword(s):  
Light Quality ◽  
Brachypodium Distachyon ◽  
Grain Dormancy ◽  
Model Grass

scholarly journals Immature embryo: A useful tool for oil palm (Elaeis guineensis Jacq.) genetic transformation studies

2005 ◽  
Vol 8 (1) ◽  
Author(s):  
Ruslan Abdullah ◽  
Alizah Zainal ◽  
Wee Yew Heng ◽  
Leaw Chui Li ◽  
Yeap Chee Beng ◽  
...  
Keyword(s):  
Genetic Transformation ◽  
Oil Palm ◽  
Elaeis Guineensis ◽  
Immature Embryo ◽  
Elaeis Guineensis Jacq

scholarly journals Centromeric DNA characterization in the model grass Brachypodium distachyon provides insights on the evolution of the genus

2018 ◽  
Vol 93 (6) ◽  
pp. 1088-1101 ◽  
Author(s):  
Yinjia Li ◽  
Sheng Zuo ◽  
Zhiliang Zhang ◽  
Zhanjie Li ◽  
Jinlei Han ◽  
...  
Keyword(s):  
Brachypodium Distachyon ◽  
Centromeric Dna ◽  
Dna Characterization ◽  
Model Grass

Embryo Development and Anatomy in Araucaria Juss

1983 ◽  
Vol 31 (2) ◽  
pp. 125 ◽  
Author(s):  
RJ Haines
Keyword(s):  
Embryo Development ◽  
Vascular Tissue ◽  
Mature Embryo ◽  
The State ◽  
Root Cap ◽  
The Other ◽  
Published Data ◽  
General Outline ◽  
Mature Embryos

Embryo development following suspensor elongation was studied in three species of Araucaria: A. cunninghamii, A. heterophylla and A. bidwillii. The mature embryos of these species were compared with those of A. hunsteinii and species for which published data are available. In general outline, the embryogeny of Araucaria resembles that described for a number of other conifers. Differences are evident among species of Araucaria with respect to the following features of the mature embryo: overall size; relative lengths of the cotyledons, hypocotyl and root cap; cotyledon number; the presence of stomates; the extent and arrangement of the vascular and secretory tissues; and the state of differentiation of the vascular tissue. Consideration of all these features lends some support to the generally recognized division of the genus into four sections, although there is some evidence to suggest a relationship between the Eutacta and Intermedia species, on one hand, and the Columbea and Bunya species, on the other.

QTLs for resistance to the false brome rust Puccinia brachypodii in the model grass Brachypodium distachyon L.

Genome ◽  
2012 ◽  
Vol 55 (2) ◽  
pp. 152-163 ◽  
Author(s):  
Mirko Barbieri ◽  
Thierry C. Marcel ◽  
Rients E. Niks ◽  
Enrico Francia ◽  
Marianna Pasquariello ◽  
...  
Keyword(s):  
Quantitative Resistance ◽  
Rust Fungus ◽  
Brachypodium Distachyon ◽  
Transgressive Segregation ◽  
Snp Markers ◽  
Growth Stages ◽  
Resistance Qtls ◽  
Ssr And Snp Markers ◽  
Model Grass ◽  
Genomic Regions

The potential of the model grass Brachypodium distachyon L. (Brachypodium) for studying grass–pathogen interactions is still underexploited. We aimed to identify genomic regions in Brachypodium associated with quantitative resistance to the false brome rust fungus Puccinia brachypodii . The inbred lines Bd3-1 and Bd1-1, differing in their level of resistance to P. brachypodii, were crossed to develop an F2 population. This was evaluated for reaction to a virulent isolate of P. brachypodii at both the seedling and advanced growth stages. To validate the results obtained on the F2, resistance was quantified in F2-derived F3 families in two experiments. Disease evaluations showed quantitative and transgressive segregation for resistance. A new AFLP-based Brachypodium linkage map consisting of 203 loci and spanning 812 cM was developed and anchored to the genome sequence with SSR and SNP markers. Three false brome rust resistance QTLs were identified on chromosomes 2, 3, and 4, and they were detected across experiments. This study is the first quantitative trait analysis in Brachypodium. Resistance to P. brachypodii was governed by a few QTLs: two acting at the seedling stage and one acting at both seedling and advanced growth stages. The results obtained offer perspectives to elucidate the molecular basis of quantitative resistance to rust fungi.

scholarly journals Brachypodium distachyon–Cochliobolus sativus Pathosystem is a New Model for Studying Plant–Fungal Interactions in Cereal Crops

2015 ◽  
Vol 105 (4) ◽  
pp. 482-489 ◽  
Author(s):  
Shaobin Zhong ◽  
Shaukat Ali ◽  
Yueqiang Leng ◽  
Rui Wang ◽  
David F. Garvin
Keyword(s):  
Molecular Mechanisms ◽  
Brachypodium Distachyon ◽  
Bipolaris Sorokiniana ◽  
Transgressive Segregation ◽  
Cochliobolus Sativus ◽  
Cereal Crops ◽  
New Model ◽  
Model Grass ◽  
Fungal Interactions ◽  
Important Disease

Cochliobolus sativus (anamorph: Bipolaris sorokiniana) causes spot blotch, common root rot, and kernel blight or black point in barley and wheat. However, little is known about the molecular mechanisms underlying the pathogenicity of C. sativus or the molecular basis of resistance and susceptibility in the hosts. This study aims to establish the model grass Brachypodium distachyon as a new model for studying plant–fungus interactions in cereal crops. Six B. distachyon lines were inoculated with five C. sativus isolates. The results indicated that all six B. distachyon lines were infected by the C. sativus isolates, with their levels of resistance varying depending on the fungal isolates used. Responses ranging from hypersensitive response-mediated resistance to complete susceptibility were observed in a large collection of B. distachyon (2n = 2x = 10) and B. hybridum (2n = 4x = 30) accessions inoculated with four of the C. sativus isolates. Evaluation of an F2 population derived from the cross between two of the B. distachyon lines, Bd1-1 and Bd3-1, with isolate Cs07-47-1 showed quantitative and transgressive segregation for resistance to C. sativus, suggesting that the resistance may be governed by quantitative trait loci from both parents. The availability of whole-genome sequences of both the host (B. distachyon) and the pathogen (C. sativus) makes this pathosystem an attractive model for studying this important disease of cereal crops.

Comparison of a high-density genetic linkage map to genome features in the model grass Brachypodium distachyon

2011 ◽  
Vol 123 (3) ◽  
pp. 455-464 ◽  
Author(s):  
Naxin Huo ◽  
David F. Garvin ◽  
Frank M. You ◽  
Stephanie McMahon ◽  
Ming-Cheng Luo ◽  
...  
Keyword(s):  
Linkage Map ◽  
Genetic Linkage ◽  
Genetic Linkage Map ◽  
Brachypodium Distachyon ◽  
High Density ◽  
Genome Features ◽  
Model Grass
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