scholarly journals Viral Expression of Epigenome Editing Tools in Rodent Brain Using Stereotaxic Surgery Techniques

2018 ◽  
pp. 205-214 ◽  
Author(s):  
Peter J. Hamilton ◽  
Carissa J. Lim ◽  
Eric J. Nestler ◽  
Elizabeth A. Heller
Keyword(s):  
Epigenome Editing ◽  
Stereotaxic Surgery ◽  
Viral Expression ◽  
Rodent Brain

scholarly journals Stereotaxic Surgery and Viral Delivery of Zinc-Finger Epigenetic Editing Tools in Rodent Brain

2018 ◽  
pp. 229-238 ◽  
Author(s):  
Peter J. Hamilton ◽  
Carissa J. Lim ◽  
Eric J. Nestler ◽  
Elizabeth A. Heller
Keyword(s):  
Zinc Finger ◽  
Stereotaxic Surgery ◽  
Rodent Brain ◽  
Epigenetic Editing

Progress of In Vivo Electroporation in the Rodent Brain

2014 ◽  
Vol 14 (3) ◽  
pp. 211-217 ◽  
Author(s):  
Xue-Feng Ding ◽  
De-Lin Ma ◽  
Qiang Zhang ◽  
Wei Peng ◽  
Ming Fan ◽  
...  
Keyword(s):  
In Vivo Electroporation ◽  
Rodent Brain

scholarly journals Lentiviral Vectors for Delivery of Gene-Editing Systems Based on CRISPR/Cas: Current State and Perspectives

Viruses ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 1288
Author(s):  
Wendy Dong ◽  
Boris Kantor
Keyword(s):  
Large Scale ◽  
Lentiviral Vector ◽  
Clinical Applications ◽  
Scale Production ◽  
Base Editing ◽  
Epigenome Editing ◽  
Vector Systems ◽  
Dividing Cells

CRISPR/Cas technology has revolutionized the fields of the genome- and epigenome-editing by supplying unparalleled control over genomic sequences and expression. Lentiviral vector (LV) systems are one of the main delivery vehicles for the CRISPR/Cas systems due to (i) its ability to carry bulky and complex transgenes and (ii) sustain robust and long-term expression in a broad range of dividing and non-dividing cells in vitro and in vivo. It is thus reasonable that substantial effort has been allocated towards the development of the improved and optimized LV systems for effective and accurate gene-to-cell transfer of CRISPR/Cas tools. The main effort on that end has been put towards the improvement and optimization of the vector’s expression, development of integrase-deficient lentiviral vector (IDLV), aiming to minimize the risk of oncogenicity, toxicity, and pathogenicity, and enhancing manufacturing protocols for clinical applications required large-scale production. In this review, we will devote attention to (i) the basic biology of lentiviruses, and (ii) recent advances in the development of safer and more efficient CRISPR/Cas vector systems towards their use in preclinical and clinical applications. In addition, we will discuss in detail the recent progress in the repurposing of CRISPR/Cas systems related to base-editing and prime-editing applications.

CRISPR/Cas9-Mediated Epigenome Editing of the IL-10 Gene for Targeted Whole Organ Gene Therapy for Lung Transplant

2021 ◽  
Vol 40 (4) ◽  
pp. S52
Author(s):  
K. Mesaki ◽  
S. Juvet ◽  
Z. Guan ◽  
J. Hu ◽  
A.R. Davidson ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Lung Transplant ◽  
Epigenome Editing

scholarly journals Histological characterization and quantification of newborn cells in the adult rodent brain

2021 ◽  
Vol 2 (3) ◽  
pp. 100614
Author(s):  
Charlotte M. Ermine ◽  
Carolina A. Chavez ◽  
Lachlan H. Thompson
Keyword(s):  
Rodent Brain

scholarly journals Wireless logging of extracellular neuronal activity in the telencephalon of free-swimming salmonids

2021 ◽  
Vol 9 (1) ◽  
Author(s):  
Susumu Takahashi ◽  
Takumi Hombe ◽  
Riku Takahashi ◽  
Kaoru Ide ◽  
Shinichiro Okamoto ◽  
...  
Keyword(s):  
Neuronal Activity ◽  
Environmental Cues ◽  
Fish Movement ◽  
Water Tank ◽  
Head Direction ◽  
Free Swimming ◽  
Rodent Brain ◽  
River Migration ◽  
Spike Waveforms ◽  
The Brain

Abstract Background Salmonids return to the river where they were born in a phenomenon known as mother-river migration. The underpinning of migration has been extensively examined, particularly regarding the behavioral correlations of external environmental cues such as the scent of the mother-river and geomagnetic compass. However, neuronal underpinning remains elusive, as there have been no biologging techniques suited to monitor neuronal activity in the brain of large free-swimming fish. In this study, we developed a wireless biologging system to record extracellular neuronal activity in the brains of free-swimming salmonids. Results Using this system, we recorded multiple neuronal activities from the telencephalon of trout swimming in a rectangular water tank. As proof of principle, we examined the activity statistics for extracellular spike waveforms and timing. We found cells firing maximally in response to a specific head direction, similar to the head direction cells found in the rodent brain. The results of our study suggest that the recorded signals originate from neurons. Conclusions We anticipate that our biologging system will facilitate a more detailed investigation into the neural underpinning of fish movement using internally generated information, including responses to external cues.

Changing concepts in the treatment of colloid cysts

1987 ◽  
Vol 66 (2) ◽  
pp. 186-191 ◽  
Author(s):  
Walter A. Hall ◽  
L. Dade Lunsford
Keyword(s):  
Third Ventricle ◽  
Ct Scanning ◽  
Morbidity Rate ◽  
Ct Guided ◽  
Content Type ◽  
Persistent Symptoms ◽  
The Third ◽  
Stereotaxic Surgery ◽  
Colloid Cysts ◽  
The University

✓ Since computerized tomography (CT) scanning became available at the University Health Center of Pittsburgh in July, 1975, 17 patients have undergone removal of colloid cysts of the third ventricle by transfrontal, transcallosal, or stereotaxic surgery. All patients presented with symptoms and signs of increased intracranial pressure; CT scanning proved to be the best neurodiagnostic test to define the colloid cysts. Since the development of CT-guided stereotaxic surgery, the authors have preferentially performed stereotaxic aspiration in seven patients; three of these subsequently required craniotomies to remove residual cysts producing persistent symptoms. The viscosity of the intracystic colloid material and/or displacement of the cyst away from the aspiration needle were reasons for unsuccessful aspiration; the CT appearance did not correlate with the ability to aspirate the lesion by the stereotaxic technique. Postoperative patency of the ventricular system was documented by intraoperative CT ventriculography performed during stereotaxic surgery. Removal of the cyst wall was not necessary. Because of the low associated morbidity rate, percutaneous stereotaxic aspiration is recommended as the initial treatment of choice for colloid cysts of the third ventricle. If stereotaxic aspiration fails and symptoms persist, craniotomy should be performed.

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