Feline Adult Adipose Tissue-Derived Multipotent Stromal Cell Isolation and Differentiation

2018 ◽  
pp. 173-188
Author(s):  
Carmel Fargason ◽  
Nan Zhang ◽  
Mandi J. Lopez
Keyword(s):  
Adipose Tissue ◽  
Stromal Cell ◽  
Cell Isolation ◽  
Multipotent Stromal Cell

A Porcine Model for Adipose Tissue-Derived Endothelial Cell Transplantation

1992 ◽  
Vol 1 (4) ◽  
pp. 293-298 ◽  
Author(s):  
Carlton Young ◽  
Bruce E. Jarrell ◽  
James B. Hoying ◽  
Stuart K. Williams
Keyword(s):  
Endothelial Cells ◽  
Adipose Tissue ◽  
Endothelial Cell ◽  
Cell Transplantation ◽  
Animal Studies ◽  
Porcine Model ◽  
Cell Isolation ◽  
Microvascular Endothelial Cell ◽  
Microvascular Endothelium ◽  
Microvascular Endothelial

The transplantation of endothelial cells represents a technology which has been suggested for applications ranging from improvement in function of implanted vascular devices to genetic therapy. The use of microvascular endothelial cell transplantation has seen increased use both in animal studies as well as clinical use. This report describes our techniques for the isolation and establishment of initial cultures of microvascular endothelial cells derived from porcine fat. A variety of anatomic sites within the pig were evaluated to determine the appropriateness of different sources of fat for endothelial cell isolation. The properitoneal fat was determined to be optimal due to the predominance of endothelium in this tissue and the ease of isolation of microvascular endothelium following collagenase digestion. The study of endothelial cell transplantation in the porcine model is now possible using the methods described for adipose tissue-derived micro vessel endothelial cell isolation.

scholarly journals Successful Use of Human AB Serum to Support the Expansion of Adipose Tissue-Derived Mesenchymal Stem/Stromal Cell in a Microcarrier-Based Platform

2020 ◽  
Vol 8 ◽  
Author(s):  
Francisco Moreira ◽  
Amanda Mizukami ◽  
Lucas Eduardo Botelho de Souza ◽  
Joaquim M. S. Cabral ◽  
Cláudia L. da Silva ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Stromal Cell

scholarly journals Changes in adipose tissue of the rat due to early undernutrition followed by rehabilitation

1980 ◽  
Vol 43 (1) ◽  
pp. 33-43 ◽  
Author(s):  
Janet Kirtland ◽  
Patricia M. Harris
Keyword(s):  
Adipose Tissue ◽  
Stromal Cell ◽  
Specific Activity ◽  
Tritiated Thymidine ◽  
Cell Replication ◽  
Fat Cell ◽  
Finite Period ◽  
Cell Fractions ◽  
Specific Radio ◽  
Deep Body

1. Well-nourished rats were injected with tritiated thymidine at 15, 22, 28 or 84 d of age. At 1, 6, 11 and 16 d after injection animals from each group were killed, samples of adipose tissue were removed from two subcutanecus sites (abdominal and scapular) and separated, using collagenase (EC 3.4.24.3), into ‘fat cell’ and ‘stromal cell’ fractions. The specific (radio)activity of DNA isolated from each fraction was measured. The specific activity of DNA isolated from two ‘deep body’ sites (perirenal and epididymal) was measured only in the animals injected at 84 d of age.2. Animals undernourished from birth up to 84 d of age were injected with tritiated thymidine at 22, 28 or 84 d of age. Animals were killed 1 and 11 d after injection, adipose tissue removed, and the specific activity of DNA measured. Other undernourished animals were rehabilitated from 84 to 107 d and injected at 91 d of age with tritiated thymidine. The animals were killed 1, 6, 11 and 16 d after injection, adipose tissue was removed from the subcutaneous and deep body sites and the specific activity of DNA determined as before.3. In well-nourished animals fat cell replication had largely ceased by 12 weeks of age in the subcutaneous depots. There were differences between the various sites of adipose tissue regarding the period of hyperplastic growth, its timing or rate of replication or both.4. In undernourished animals replication was slow in the subcutaneous depots compared with well-nourished animals of the same age. Rehabilitation from undernutrition stimulated replication which resulted in higher rates in all four depots examined compared with those in well-nourished animals.5. The findings are discussed in relation to the concept of a finite period of hyperplasia for adipose tissue.

scholarly journals Peptide Nanofiber Scaffolds for Multipotent Stromal Cell Culturing

2013 ◽  
pp. 61-76
Author(s):  
Seher Ustun ◽  
Samet Kocabey ◽  
Mustafa O. Guler ◽  
Ayse B. Tekinay
Keyword(s):  
Stromal Cell ◽  
Multipotent Stromal Cell ◽  
Cell Culturing ◽  
Nanofiber Scaffolds

scholarly journals Human Multipotent Stromal Cell Secreted Effectors Accelerate Islet Regeneration

Stem Cells ◽  
2019 ◽  
Vol 37 (4) ◽  
pp. 516-528 ◽  
Author(s):  
Miljan Kuljanin ◽  
Ruth M. Elgamal ◽  
Gillian I. Bell ◽  
Anargyros Xenocostas ◽  
Gilles A. Lajoie ◽  
...  
Keyword(s):  
Stromal Cell ◽  
Islet Regeneration ◽  
Multipotent Stromal Cell

scholarly journals New Mechanical Fat Separation Technique: Adjustable Regenerative Adipose-tissue Transfer (ARAT) and Mechanical Stromal Cell Transfer (MEST)

2020 ◽  
Vol 2 (4) ◽  
Author(s):  
H Eray Copcu ◽  
Sule Oztan
Keyword(s):  
Adipose Tissue ◽  
Stromal Cells ◽  
Stromal Cell ◽  
Retention Rate ◽  
High Rate ◽  
Cell Transfer ◽  
Fat Tissue ◽  
Cell Counts ◽  
Tissue Transfer ◽  
Regenerative Cells

Abstract Background Adipose tissue is not only a very important source of filler but also the body’s greatest source of regenerative cells. Objectives In this study, adipose tissue was cut to the desired dimensions using ultra-sharp blade systems to avoid excessive blunt pressure and applied to various anatomical areas—a procedure known as adjustable regenerative adipose-tissue transfer (ARAT). Mechanical stromal cell transfer (MEST) of regenerative cells from fat tissue was also examined. Methods ARAT, MEST, or a combination of these was applied in the facial area of a total of 24 patients who were followed for at least 24 months. The integrity of the fat tissue cut with different diameter blades is shown histopathologically. The number and viability of the stromal cells obtained were evaluated and secretome analyses were performed. Patient and surgeon satisfaction were assessed with a visual analog scale. Results With the ARAT technique, the desired size fat grafts were obtained between 4000- and 200-micron diameters and applied at varying depths to different aesthetic units of the face, and a guide was developed. In MEST, stromal cells were obtained from 100 mL of condensed fat using different indication-based protocols with 93% mean viability and cell counts of 28.66 to 88.88 × 106. Conclusions There are 2 main complications in fat grafting: visibility in thin skin and a low retention rate. The ARAT technique can be used to prevent these 2 complications. MEST, on the other hand, obtains a high rate of fat and viable stromal cells without applying excessive blunt pressure. Level of Evidence: 4

scholarly journals Th17 Pathway As a Target for Multipotent Stromal Cell Therapy in Dogs: Implications for Translational Research

PLoS ONE ◽  
2016 ◽  
Vol 11 (2) ◽  
pp. e0148568 ◽  
Author(s):  
A. Kol ◽  
N. J. Walker ◽  
M. Nordstrom ◽  
D. L. Borjesson
Keyword(s):  
Cell Therapy ◽  
Translational Research ◽  
Stromal Cell ◽  
Multipotent Stromal Cell
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