Reassembly of Functional Human Stem/Progenitor Cells in 3D Culture

2018 ◽  
pp. 19-32 ◽  
Author(s):  
Danielle Wu ◽  
Patricia Chapela ◽  
Mary C. Farach-Carson
Keyword(s):  
Progenitor Cells ◽  
3D Culture

scholarly journals A 3D culture system enhances the ability of human bone marrow stromal cells to support the growth of limbal stem/progenitor cells

2016 ◽  
Vol 16 (2) ◽  
pp. 358-364 ◽  
Author(s):  
Sheyla González ◽  
Hua Mei ◽  
Martin N. Nakatsu ◽  
Elfren R. Baclagon ◽  
Sophie X. Deng
Keyword(s):  
Bone Marrow ◽  
Progenitor Cells ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Culture System ◽  
3D Culture ◽  
Marrow Stromal Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
3D Culture System

scholarly journals Comparative study of visible light polymerized gelatin hydrogels for 3D culture of hepatic progenitor cells

2016 ◽  
Vol 134 (11) ◽  
Author(s):  
Tanja Greene ◽  
Tsai-Yu Lin ◽  
Ourania M. Andrisani ◽  
Chien-Chi Lin
Keyword(s):  
Comparative Study ◽  
Visible Light ◽  
Progenitor Cells ◽  
3D Culture ◽  
Hepatic Progenitor Cells

scholarly journals Keratin Profiling by Single-Cell RNA-Sequencing Identifies Human Prostate Stem Cell Lineage Hierarchy and Cancer Stem-Like Cells

2021 ◽  
Vol 22 (15) ◽  
pp. 8109
Author(s):  
Wen-Yang Hu ◽  
Dan-Ping Hu ◽  
Lishi Xie ◽  
Larisa Nonn ◽  
Ranli Lu ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Stem Cells ◽  
Stem Cell ◽  
Single Cell ◽  
Progenitor Cells ◽  
3D Culture ◽  
Cell Lineage ◽  
Gene Set Enrichment Analysis ◽  
Human Prostate ◽  
Stem Cell Lineage

Single prostate stem cells can generate stem and progenitor cells to form prostaspheres in 3D culture. Using a prostasphere-based label retention assay, we recently identified keratin 13 (KRT13)-enriched prostate stem cells at single-cell resolution, distinguishing them from daughter progenitors. Herein, we characterized the epithelial cell lineage hierarchy in prostaspheres using single-cell RNA-seq analysis. Keratin profiling revealed three clusters of label-retaining prostate stem cells; cluster I represents quiescent stem cells (PSCA, CD36, SPINK1, and KRT13/23/80/78/4 enriched), while clusters II and III represent active stem and bipotent progenitor cells (KRT16/17/6 enriched). Gene set enrichment analysis revealed enrichment of stem and cancer-related pathways in cluster I. In non-label-retaining daughter progenitor cells, three clusters were identified; cluster IV represents basal progenitors (KRT5/14/6/16 enriched), while clusters V and VI represent early and late-stage luminal progenitors, respectively (KRT8/18/10 enriched). Furthermore, MetaCore analysis showed enrichment of the “cytoskeleton remodeling–keratin filaments” pathway in cancer stem-like cells from human prostate cancer specimens. Along with common keratins (KRT13/23/80/78/4) in normal stem cells, unique keratins (KRT10/19/6C/16) were enriched in cancer stem-like cells. Clarification of these keratin profiles in human prostate stem cell lineage hierarchy and cancer stem-like cells can facilitate the identification and therapeutic targeting of prostate cancer stem-like cells.

Maintenance and Expansion of Hematopoietic Stem/Progenitor Cells in a Biomimetic Hematopoietic Cells Niche.

Blood ◽  
2007 ◽  
Vol 110 (11) ◽  
pp. 4115-4115
Author(s):  
Ting Liu ◽  
Jing Tan ◽  
Li Hou ◽  
Wentong Meng ◽  
Yuchun Wang
Keyword(s):  
Progenitor Cells ◽  
Culture System ◽  
Three Dimensional ◽  
3D Culture ◽  
Hematopoietic Progenitor Cells ◽  
Hematopoietic Progenitor ◽  
Hematopoietic Stem ◽  
Cd34 Cells ◽  
3D Culture System ◽  
2D System

Abstract As main hematopoietic organ, bone marrow have three dimensional microenvironment for hematopoietic stem/progenitor cells grow in, so call “hematopoietic cells niche”, which are composed by stromal cells and extracelluar matrix. The interactions of cell to cell and cell to matrix between stem/progenitor cells with hematopoietic niche are facilitated by its three dimensional conformation. The biology behaviors of hematopoietic stem cells are mediate by many signal transductions between stem/progenitor cells with their corresponding microenvironment. Now there are strong evidence from animal model study suggests that osteoblasts might play an essential role in creation of a hematopoietic stem cell niche and thereby regulation of stem cell maintenance, proliferation, and maturation. In light of the structure-function relationship of bone marrow topography, we conceived a biomimetic culture system (3D culture system) with bio-derived bone as framework, composited with human marrow mesenchymal stem cells, and induced the cells into osteoblasts to simulate the effects of hematopoietic osteobalst niche. CD 34+ cells or mononuclear cells separated from umbilical cord blood were cultured for 2∼5 weeks in the 3D culture system and also in a conventional 2D culture system as control without additional cytokine supplement. Based on our results, higher expression of extracelluar matrix and N-cadherin were observed in 3D culture system compared to 2D system. At 2 weeks culture, 3D culture system showed higher number of CD34+ cells and CD34+/CD38- cells when compared with the input (P<0.05), the increased cells were predominant CD34+/CD38-cells. Although CD34+ cells were decreased at 5 weeks culture; nevertheless, CD34+/CD38- cells were still maintained at high level. We also observed that imbedding MNCs with a higher percentage of CD34+/CD38-cells cultured in 3D system (P<0.05), which may represent a down regulation of CD38 phenotype during culture. The function of cultured cells was evaluated in colony forming unit (CFU) assay and long term culture initial cell (LTC-IC) assay. 3D system produced higher expansion of CFU progenitors than 2D system (7.13–8.89 times vs. 1.22–1.31times) after 2 weeks culture. Of note, the colony distribution of 3D system manifested higher percentage of BFU-E and CFU-GEMM, while 2D system showed mainly CFU-GM. LTC-IC represents the primitive progenitor, 3D system showed a 6.2 times increment over input after 2 weeks culture. Furthermore, it was competent to maintain the immaturation of hematopoietic progenitor cells (HPCs) over 5 weeks. This study demonstrated that our 3D culture system constructed with the bio-derived bone composited with induced osteoblast is capable to allow maintenance and expansion of primitive hematopoietic progenitor cells in vitro. It may open a new avenue to study HSCs/HPCs behaviors and to achieve sustained primitive progenitor cell expansion.

scholarly journals Activation of the RhoA-YAP-β-catenin signaling axis promotes the expansion of inner ear progenitor cells in 3D culture

Stem Cells ◽  
2020 ◽  
Author(s):  
Mingyu Xia ◽  
Yan Chen ◽  
Yingzi He ◽  
Huawei Li ◽  
Wenyan Li
Keyword(s):  
Inner Ear ◽  
Progenitor Cells ◽  
3D Culture ◽  
Signaling Axis

Abstract 3435: Profiling miRNAs in breast epithelial progenitor cells during branching morphogenesis and EMT in 3D culture

2017 ◽  
Author(s):  
Eirikur Briem ◽  
Bylgja Hilmarsdottir ◽  
Zuzana Budkova ◽  
Saevar Ingthorsson ◽  
Magnus K. Magnusson ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
3D Culture ◽  
Branching Morphogenesis ◽  
Breast Epithelial ◽  
Epithelial Progenitor Cells

PS21 - 103. Multipotent pancreatic progenitor cells in 3D culture of human pancreatic ductal tissue: potential relevance for beta cell therapy in Type 1 diabetes

2012 ◽  
Vol 10 (3) ◽  
pp. 172-172
Author(s):  
Nerys A. Williams ◽  
Cindy J.M. Loomans ◽  
Leon C.A. van Gurp ◽  
Femke C.A.S. Ringnalda ◽  
Toshiro Sato ◽  
...  
Keyword(s):  
Type 1 Diabetes ◽  
Cell Therapy ◽  
Beta Cell ◽  
Progenitor Cells ◽  
3D Culture ◽  
Pancreatic Progenitor ◽  
Pancreatic Progenitor Cells ◽  
Ductal Tissue

Regulatory effect of dimethylarginine dimethylaminohydrolase on endothelial progenitor cells differentiation and function

2010 ◽  
Vol 34 (8) ◽  
pp. S42-S42
Author(s):  
Qiong Yuan ◽  
Chang‑Ping Hu ◽  
Si‑Yu Liu ◽  
Xu‑Meng Chen ◽  
Jun Peng ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Regulatory Effect ◽  
Dimethylarginine Dimethylaminohydrolase ◽  
Endothelial Progenitor ◽  
And Function
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