Isolation and Characterization of Extracellular Vesicles from Mesenchymal Stromal Cells

2019 ◽  
pp. 15-23 ◽  
Author(s):  
Sally Yunsun Kim ◽  
Thanh Huyen Phan ◽  
Christina Limantoro ◽  
Bill Kalionis ◽  
Wojciech Chrzanowski
Keyword(s):  
Extracellular Vesicles ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Isolation And Characterization

scholarly journals Isolation and characterization of multipotent mesenchymal stromal cells from the gingiva and the periodontal ligament of the horse

2011 ◽  
Vol 7 (1) ◽  
pp. 42 ◽  
Author(s):  
Niels Mensing ◽  
Hagen Gasse ◽  
Nina Hambruch ◽  
Jan-Dirk Haeger ◽  
Christiane Pfarrer ◽  
...  
Keyword(s):  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Periodontal Ligament ◽  
Multipotent Mesenchymal Stromal Cells ◽  
Isolation And Characterization

scholarly journals Isolation and Characterization of Multipotential Mesenchymal Stromal Cells from Congenital Pseudoarthrosis of the Tibia: Case Report

2015 ◽  
Vol 298 (10) ◽  
pp. 1804-1814 ◽  
Author(s):  
Dylana Diaz-Solano ◽  
Olga Wittig ◽  
Jose D. Mota ◽  
Jose E. Cardier
Keyword(s):  
Case Report ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Isolation And Characterization

scholarly journals Isolation and Characterization of Mesenchymal Stromal Cells Derived from Paediatric Patients with B Acute Lymphoblastic Leukemia

Blood ◽  
2015 ◽  
Vol 126 (23) ◽  
pp. 4771-4771
Author(s):  
Erica Dander ◽  
Chiara Palmi ◽  
Federica Portale ◽  
Linda Beneforti ◽  
Andrea Biondi ◽  
...  
Keyword(s):  
Acute Lymphoblastic Leukemia ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Lymphoblastic Leukemia ◽  
Chromosomal Translocations ◽  
Leukemia Cells ◽  
Alizarin Red ◽  
Differentiation Potential ◽  
Isolation And Characterization

Abstract PURPOSE: Several studies reported the importance of tumor microenvironment in the development and progression of hematological disorders. The identification of key factors involved in the crosstalk between the malignant cells and the bone marrow Mesenchymal Stromal Cells (BM-MSCs) may provide a tool for interfering with the protective BM niche. The purpose of our work was to isolate and characterize morphologically, phenotypically and functionally MSCs derived from pediatric patients with B Acute Lymphoblastic Leukemia (B-ALL). METHODS: MSCs were isolated from BM-MNCs obtained from 10 B-ALL children (n=5 non-traslocated high risk patients and n=5 t(12;21) patients) and from 6 healthy donors (HDs) and cultured in DMEM 10% FCS. MSCs were characterized at fourth passage in terms of morphology, immunophenotype (FACS analysis) and in vitro adipogenic and osteogenic differentiation potential. Chromosomal translocations detected in leukemia cells were investigated in B-ALL-MSCs by fluorescence in situ hybridizations (FISH) or polymerase chain reaction (PCR). RESULTS: Both HD-MSCs and B-ALL-MSCs resulted comparable in terms of morphology. They both expressed the typical MSC markers CD73, CD90 and CD105, while lacked the expression of the hematopoietic markers CD14, CD34, CD45 and MHC-II. HD-MSCs as well as B-ALL-MSCs were able to differentiate, under appropriate stimuli, into adipogenic and osteogenic lineages as showed by Oil Red O liphophilic dye and Alizarin Red staining of calcium deposits. In addition, MSC from all investigated ALL patients did not present the chromosomal translocations that had been detected in leukemia cells (1 patient BCR-ABL p210, 5 patients TEL-AML1). CONCLUSIONS: We found that B-ALL-MSCs resulted similar in terms of morphology, phenotype and differentiation ability to HD-MSCs. Furthermore, MSCs from patients did not reveal the chromosomal translocations present in leukemia blasts. Functional characterization of MSCs in terms of soluble molecule production is needed to identify altered cellular pathways. Since emerging evidence supports the importance of the MSCs in the leukemic niche, we will focus on the potential functional alterations of ALL-MSCs. Our purpose is to understand the mechanisms underlying the support of leukemic cells by the BM microenvironment. The discovery of altered molecular pathways will pave the way for the development of new immunotherapy strategies for targeting the leukemic niche. Disclosures No relevant conflicts of interest to declare.

Isolation and characterization of CD146+ multipotent mesenchymal stromal cells

2008 ◽  
Vol 36 (8) ◽  
pp. 1035-1046 ◽  
Author(s):  
Antonio Sorrentino ◽  
Manuela Ferracin ◽  
Germana Castelli ◽  
Mauro Biffoni ◽  
Giada Tomaselli ◽  
...  
Keyword(s):  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Multipotent Mesenchymal Stromal Cells ◽  
Isolation And Characterization

scholarly journals Isolation and characterization of equine endometrial mesenchymal stromal cells

2017 ◽  
Vol 8 (1) ◽  
Author(s):  
B. Elisabeth Rink ◽  
Karin R. Amilon ◽  
Cristina L. Esteves ◽  
Hilari M. French ◽  
Elaine Watson ◽  
...  
Keyword(s):  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Isolation And Characterization
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