Monitoring the Cerebrospinal Fluid Cytokine Profile Using Membrane-Based Antibody Arrays

2019 ◽  
pp. 233-246
Author(s):  
Andrea González-Morales ◽  
Mercedes Lachén-Montes ◽  
Joaquín Fernández-Irigoyen ◽  
Enrique Santamaría
Keyword(s):  
Cerebrospinal Fluid ◽  
Cytokine Profile ◽  
Antibody Arrays

scholarly journals Cytokine Profiling of Macrophages Exposed to Porphyromonas gingivalis, Its Lipopolysaccharide, or Its FimA Protein

2005 ◽  
Vol 73 (2) ◽  
pp. 935-943 ◽  
Author(s):  
Qingde Zhou ◽  
Tesfahun Desta ◽  
Matthew Fenton ◽  
Dana T. Graves ◽  
Salomon Amar
Keyword(s):  
Porphyromonas Gingivalis ◽  
Peritoneal Macrophages ◽  
Cytokine Profile ◽  
Enzyme Linked Immunosorbent Assay ◽  
Necrosis Factor Alpha ◽  
Monocyte Chemoattractant Protein 1 ◽  
Antibody Arrays ◽  
Mouse Peritoneal Macrophages

ABSTRACT To characterize the roles of Porphyromonas gingivalis and its components in the disease processes, we investigated the cytokine profile induced by live P. gingivalis, its lipopolysaccharides (LPS), and its major fimbrial protein, fimbrillin (FimA). Using cytokine antibody arrays, we found that P. gingivalis LPS and FimA induced a similar profile of cytokine expression when exposed to mouse peritoneal macrophages but that this profile differed significantly in response to live P. gingivalis. In vitro, mouse peritoneal macrophages were stimulated to produce interleukin-6 (IL-6), granulocyte colony-stimulating factor, and lymphotactin by live P. gingivalis, but not by P. gingivalis LPS or FimA, while RANTES, gamma interferon, IL-17, vascular cell adhesion molecule 1 (VCAM-1), and vascular endothelial growth factor were induced by P. gingivalis LPS or FimA, but not by live P. gingivalis. In vivo, IL-6 mRNA was strongly induced only by live P. gingivalis while monocyte chemoattractant protein 1 mRNA was strongly induced only by P. gingivalis LPS and FimA in mouse calvarial scalp, further confirming the differences of cytokine profile induced in vitro. Cytokine antibody arrays using toll-like receptor 2 (TLR2)- and TLR4-deficient macrophages revealed that most of the cytokines induced by P. gingivalis LPS or FimA signal through TLR2, while most of cytokines induced by live P. gingivalis signal through both TLR2 and TLR4. Interestingly, the activation of TLR2 by live P. gingivalis inhibited the release of RANTES, VCAM-1, and IL-1α from mouse peritoneal macrophages. A tumor necrosis factor alpha enzyme-linked immunosorbent assay further confirmed that P. gingivalis LPS and FimA activate mouse peritoneal macrophages via TLR2. These results indicate that host immune cells sense live P. gingivalis and its components differently, which translates into the expression of different inflammatory cytokine profiles.

ALS: Cytokine profile in cerebrospinal fluid T‐cell clones

2006 ◽  
Vol 7 (3) ◽  
pp. 183-186 ◽  
Author(s):  
Trygve Holmøy ◽  
Per M. Roos ◽  
Espen Østhagen Kvale
Keyword(s):  
Cerebrospinal Fluid ◽  
T Cell ◽  
Cytokine Profile ◽  
T Cell Clones ◽  
Cell Clones

scholarly journals Intravenous Immunoglobulins in Refractory Childhood-Onset Epilepsy: Effects on Seizure Frequency, EEG Activity, and Cerebrospinal Fluid Cytokine Profile

Epilepsia ◽  
2007 ◽  
Vol 48 (9) ◽  
pp. 1739-1749 ◽  
Author(s):  
An D. Billiau ◽  
Peter Witters ◽  
Berten Ceulemans ◽  
Ahmad Kasran ◽  
Carine Wouters ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Seizure Frequency ◽  
Intravenous Immunoglobulins ◽  
Cytokine Profile ◽  
Childhood Onset ◽  
Eeg Activity

The Th1 versus Th2 cytokine profile in cerebrospinal fluid after severe traumatic brain injury in infants and children

2001 ◽  
Vol 2 (3) ◽  
pp. 260-264 ◽  
Author(s):  
Jonathan E. Amick ◽  
Kristin A. Yandora ◽  
Michael J. Bell ◽  
Stephen R. Wisniewski ◽  
P. David Adelson ◽  
...  
Keyword(s):  
Traumatic Brain Injury ◽  
Cerebrospinal Fluid ◽  
Brain Injury ◽  
Severe Traumatic Brain Injury ◽  
Cytokine Profile ◽  
Infants And Children ◽  
Th2 Cytokine ◽  
In Infants And Children

A pilot study of cytokine profile in cerebrospinal fluid of children with acute lymphocytic leukemia and neurotoxic side effects of chemotherapy

2021 ◽  
Vol 67 (4) ◽  
pp. 374-377
Author(s):  
V.V. Bazarnyi ◽  
O.P. Kovtun ◽  
O.V. Koryakina ◽  
L.G. Polushina ◽  
A.Yu. Maksimova
Keyword(s):  
Cerebrospinal Fluid ◽  
Side Effects ◽  
Acute Lymphocytic Leukemia ◽  
Comparison Group ◽  
Neurological Complications ◽  
Cytokine Profile ◽  
Lymphocytic Leukemia ◽  
Standard Chemotherapy ◽  
Chemokine Cxcl12 ◽  
Xmap Technology

In some cases standard chemotherapy of acute lymphocytic leukemia (ALL) leads to neurotoxicity; its mechanisms, methods of prognosis, and prevention are being actively studied. The aim of this study was to assess the cytokine profile in cerebrospinal fluid (CSF) of children with ALL and neurotoxic side effects of chemotherapy. This prospective study included 24 children with ALL aged from 3 to 17 years. Patients were further subdivided into ALL patients with (main group) and without neurological complications (comparison group). The level of cytokines in CSF was measured by Xmap technology (Luminex) using Invitrogen test systems (eBioscience) and the Luminex 200 system. The comparative analysis of the cytokine profile in the group of children with chemotherapy-induced neurotoxic complications revealed elevated levels of chemokine CXCL12 (SDF-1α) and stem cell factor (SCF). Increased level of these cytokines in CSF was characterized by a relatively risk for development of toxic peripheral neuropathy.

Serum and cerebrospinal fluid cytokine profile of patients with 2009 pandemic H1N1 influenza virus-associated encephalopathy

Cytokine ◽  
2011 ◽  
Vol 54 (2) ◽  
pp. 167-172 ◽  
Author(s):  
Shunji Hasegawa ◽  
Takeshi Matsushige ◽  
Hirofumi Inoue ◽  
Komei Shirabe ◽  
Reiji Fukano ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Influenza Virus ◽  
H1n1 Influenza ◽  
Cytokine Profile ◽  
Pandemic H1n1 ◽  
H1n1 Influenza Virus ◽  
Pandemic H1n1 Influenza
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