Live Cell Imaging of Neural Stem Cells in the Drosophila Larval Brain

2019 ◽  
pp. 153-160
Author(s):  
Karolina Miszczak ◽  
Boris Egger
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Larval Brain

scholarly journals ANGI-03OBSERVATION OF GLIOMA STEM CELLS TRANSFORM INTO ENDOTHELIAL CELLS VIA LIVE CELL IMAGING

2015 ◽  
Vol 17 (suppl 5) ◽  
pp. v41.3-v41
Author(s):  
Xin Mei ◽  
Yinsheng Chen ◽  
Zhongping Chen
Keyword(s):  
Stem Cells ◽  
Endothelial Cells ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Glioma Stem Cells

scholarly journals Live Cell Imaging of the Nascent Inactive X Chromosome during the Early Differentiation Process of Naive ES Cells towards Epiblast Stem Cells

PLoS ONE ◽  
2014 ◽  
Vol 9 (12) ◽  
pp. e116109 ◽  
Author(s):  
Aurélia Guyochin ◽  
Sylvain Maenner ◽  
Erin Tsi-Jia Chu ◽  
Asma Hentati ◽  
Mikael Attia ◽  
...  
Keyword(s):  
Stem Cells ◽  
X Chromosome ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Es Cells ◽  
Live Cell ◽  
Differentiation Process ◽  
Early Differentiation ◽  
Epiblast Stem Cells ◽  
Inactive X Chromosome

scholarly journals Dynamics of CTCF and cohesin mediated chromatin looping revealed by live-cell imaging

2021 ◽  
Author(s):  
Michele Gabriele ◽  
Hugo B Brandão ◽  
Simon Grosse-Holz ◽  
Asmita Jha ◽  
Gina M Dailey ◽  
...  
Keyword(s):  
Stem Cells ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Embryonic Stem ◽  
Super Resolution ◽  
Live Cell ◽  
Chromatin Looping ◽  
Topologically Associating Domains ◽  
Dynamic View ◽  
Animal Genomes

Animal genomes are folded into loops and topologically associating domains (TADs) by CTCF and cohesin, but whether these loops are stable or dynamic is unknown. Here, we directly visualize chromatin looping at the Fbn2 TAD in mouse embryonic stem cells using super-resolution live-cell imaging and quantify looping dynamics by Bayesian inference. Our results are consistent with cohesin-mediated loop extrusion in cells, and with CTCF both stopping and stabilizing cohesin. Surprisingly, the Fbn2 loop is both rare and dynamic, with a looped fraction of ~3-6.5% and a median loop lifetime of ~10-30 minutes. Instead of a stable loop, our results establish a highly dynamic view of TADs and loops where the Fbn2 TAD exists predominantly in a partially extruded conformation. This dynamic and quantitative view of TADs may facilitate a mechanistic understanding of their functions.

scholarly journals Live-cell imaging of subcellular structures for quantitative evaluation of pluripotent stem cells

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Ken Nishimura ◽  
Hiroshi Ishiwata ◽  
Yuta Sakuragi ◽  
Yohei Hayashi ◽  
Aya Fukuda ◽  
...  
Keyword(s):  
Stem Cells ◽  
Quantitative Evaluation ◽  
Pluripotent Stem Cells ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell

Tracking Stem Cell Function with Computers Via Live Cell Imaging: Identifying Donor Variability in Human Stem Cells

2010 ◽  
Vol 20 (2) ◽  
pp. 127-135 ◽  
Author(s):  
Bridget M. Deasy ◽  
Steven M. Chirieleison ◽  
Ashley M. Witt ◽  
Matthew J. Peyton ◽  
Taylor A. Bissell
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Cell Function ◽  
Live Cell ◽  
Human Stem Cells ◽  
Donor Variability

scholarly journals rBC2LCN, a new probe for live cell imaging of human pluripotent stem cells

2013 ◽  
Vol 431 (3) ◽  
pp. 524-529 ◽  
Author(s):  
Yasuko Onuma ◽  
Hiroaki Tateno ◽  
Jun Hirabayashi ◽  
Yuzuru Ito ◽  
Makoto Asashima
Keyword(s):  
Stem Cells ◽  
Pluripotent Stem Cells ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Human Pluripotent Stem Cells ◽  
Live Cell
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