Live-Cell Imaging of Human Pluripotent Stem Cells by a Novel Lectin Probe rBC2LCN

2014 ◽  
pp. 313-318 ◽  
Author(s):  
Hiroaki Tateno ◽  
Yasuko Onuma ◽  
Yuzuru Ito
Keyword(s):  
Stem Cells ◽  
Pluripotent Stem Cells ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Human Pluripotent Stem Cells ◽  
Live Cell

scholarly journals rBC2LCN, a new probe for live cell imaging of human pluripotent stem cells

2013 ◽  
Vol 431 (3) ◽  
pp. 524-529 ◽  
Author(s):  
Yasuko Onuma ◽  
Hiroaki Tateno ◽  
Jun Hirabayashi ◽  
Yuzuru Ito ◽  
Makoto Asashima
Keyword(s):  
Stem Cells ◽  
Pluripotent Stem Cells ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Human Pluripotent Stem Cells ◽  
Live Cell

scholarly journals Live-cell imaging of subcellular structures for quantitative evaluation of pluripotent stem cells

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Ken Nishimura ◽  
Hiroshi Ishiwata ◽  
Yuta Sakuragi ◽  
Yohei Hayashi ◽  
Aya Fukuda ◽  
...  
Keyword(s):  
Stem Cells ◽  
Quantitative Evaluation ◽  
Pluripotent Stem Cells ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell

scholarly journals ANGI-03OBSERVATION OF GLIOMA STEM CELLS TRANSFORM INTO ENDOTHELIAL CELLS VIA LIVE CELL IMAGING

2015 ◽  
Vol 17 (suppl 5) ◽  
pp. v41.3-v41
Author(s):  
Xin Mei ◽  
Yinsheng Chen ◽  
Zhongping Chen
Keyword(s):  
Stem Cells ◽  
Endothelial Cells ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Glioma Stem Cells

scholarly journals Live Cell Imaging of the Nascent Inactive X Chromosome during the Early Differentiation Process of Naive ES Cells towards Epiblast Stem Cells

PLoS ONE ◽  
2014 ◽  
Vol 9 (12) ◽  
pp. e116109 ◽  
Author(s):  
Aurélia Guyochin ◽  
Sylvain Maenner ◽  
Erin Tsi-Jia Chu ◽  
Asma Hentati ◽  
Mikael Attia ◽  
...  
Keyword(s):  
Stem Cells ◽  
X Chromosome ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Es Cells ◽  
Live Cell ◽  
Differentiation Process ◽  
Early Differentiation ◽  
Epiblast Stem Cells ◽  
Inactive X Chromosome

Live‐Cell Immunofluorescence Staining of Human Pluripotent Stem Cells

2011 ◽  
Vol 19 (1) ◽  
Author(s):  
Philip D. Manos ◽  
Sutheera Ratanasirintrawoot ◽  
Sabine Loewer ◽  
George Q. Daley ◽  
Thorsten M. Schlaeger
Keyword(s):  
Stem Cells ◽  
Pluripotent Stem Cells ◽  
Human Pluripotent Stem Cells ◽  
Live Cell ◽  
Immunofluorescence Staining

scholarly journals Development Of A Human Pluripotency Sensor For Real-Time Cell Imaging And Biomedical Applications

2020 ◽  
Vol 6 (6) ◽  
pp. 1-4
Author(s):  
Jeong Beom Kim ◽  
Keyword(s):  
Stem Cells ◽  
Regenerative Medicine ◽  
Clinical Research ◽  
Pluripotent Stem Cells ◽  
Live Cell Imaging ◽  
Biomedical Applications ◽  
Cell Imaging ◽  
Imaging Systems ◽  
Cell Based Therapy ◽  
Cell Source

uman Pluripotent Stem Cells (hPSCs) are an essential cell source for regenerative medicine. With the increasing importance of hPSCs for cell-based therapy, the need for hPSCs in basic and clinical research is to develop live-cell imaging systems that monitor hPSCs during reprogramming or differentiation processes

scholarly journals Dynamics of CTCF and cohesin mediated chromatin looping revealed by live-cell imaging

2021 ◽  
Author(s):  
Michele Gabriele ◽  
Hugo B Brandão ◽  
Simon Grosse-Holz ◽  
Asmita Jha ◽  
Gina M Dailey ◽  
...  
Keyword(s):  
Stem Cells ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Embryonic Stem ◽  
Super Resolution ◽  
Live Cell ◽  
Chromatin Looping ◽  
Topologically Associating Domains ◽  
Dynamic View ◽  
Animal Genomes

Animal genomes are folded into loops and topologically associating domains (TADs) by CTCF and cohesin, but whether these loops are stable or dynamic is unknown. Here, we directly visualize chromatin looping at the Fbn2 TAD in mouse embryonic stem cells using super-resolution live-cell imaging and quantify looping dynamics by Bayesian inference. Our results are consistent with cohesin-mediated loop extrusion in cells, and with CTCF both stopping and stabilizing cohesin. Surprisingly, the Fbn2 loop is both rare and dynamic, with a looped fraction of ~3-6.5% and a median loop lifetime of ~10-30 minutes. Instead of a stable loop, our results establish a highly dynamic view of TADs and loops where the Fbn2 TAD exists predominantly in a partially extruded conformation. This dynamic and quantitative view of TADs may facilitate a mechanistic understanding of their functions.

scholarly journals Sulfonated rhodamines as impermeable labelling substrates for cell surface protein visualization

2021 ◽  
Author(s):  
Ramona Birke ◽  
Julia Ast ◽  
Dorien A. Roosen ◽  
Bettina Mathes ◽  
Kilian Rossmann ◽  
...  
Keyword(s):  
Stem Cells ◽  
Pluripotent Stem Cells ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Surface Protein ◽  
Cell Surface Protein ◽  
Xanthene Dyes ◽  
Induced Pluripotent ◽  
Protein Tags ◽  
Human Ipsc

Sulfonated rhodamines that endow xanthene dyes with cellular impermeability are presented. We fuse charged sulfonates to red and far-red dyes to obtain Sulfo549 and Sulfo646, respectively, and further link these to SNAP- and Halo-tag substrates for protein self-labelling. Cellular impermeability is validated in live cell imaging experiments in transfected HEK cells and neurons derived from induced pluripotent stem cells (iPSCs). Lastly, we show that Sulfo646 is amenable to STED nanoscopy by recording membranes of SNAP/Halo-surface-labelled human iPSC-derived neuronal axons. We therefore provide an avenue for rendering dyes impermeable for exclusive extracellular visualization via self-labelling protein tags.

Sign in / Sign up

Export Citation Format

Share Document