Paper Chromatography of Amino Acid

2020 ◽  
pp. 259-261
Author(s):  
Aakanchha Jain ◽  
Richa Jain ◽  
Sourabh Jain
Keyword(s):  
Amino Acid ◽  
Paper Chromatography

THE OPTICAL RESOLUTION OF DL-AMINO ACID MENTHYL ESTER DERIVATIVES BY PAPER CHROMATOGRAPHY

1972 ◽  
Vol 1 (4) ◽  
pp. 269-272
Author(s):  
Isamu Akiyama ◽  
Masaji Onaya ◽  
Akira Hayakawa ◽  
Yojiro Tsuzuki
Keyword(s):  
Amino Acid ◽  
Paper Chromatography ◽  
Optical Resolution

ISOLATION AND STRUCTURE OF URIDINE NUCLEOTIDE-PEPTIDES FROM AEROBACTER CLOACAE NRC 492

1963 ◽  
Vol 41 (1) ◽  
pp. 1065-1072 ◽  
Author(s):  
R. A. Anwar ◽  
C. Roy ◽  
R. W. Watson
Keyword(s):  
Lactic Acid ◽  
Amino Acid ◽  
Lithium Chloride ◽  
Paper Chromatography ◽  
High Voltage ◽  
Chloride Solutions ◽  
Uridine Nucleotide ◽  
Ethanol Extracts ◽  
Dowex 1 ◽  
High Voltage Electrophoresis

Four closely related uridine nucleotide-peptides isolated from ethanol extracts of penicillin-treated Aerobacter cloacae NRC 492 have been partially characterized. Fractionation of dialyzed extracts on Dowex-1-Cl columns with aqueous lithium chloride solutions, precipitation of the nucleotide-peptides with methanol–acetone, and further separation by paper chromatography and high voltage electrophoresis yielded (a) UDP-GNAc-lact.L-ala.D-glu.meso-dap.D-ala,* (b) UDP-GNAc-lact.L-ala.D-glu.meso-dap, (c) UDP-GNAc-lact.L-ala.D-glu., and (d) UDP-GNAc-lactic acid. The amino acid isomers were identified by micro-enzymic and chromatographic methods.

A RAPID ULTRAFILTRATION APPARATUS

1952 ◽  
Vol 30 (3) ◽  
pp. 246-249
Author(s):  
I. W. Coleman
Keyword(s):  
Heavy Metal ◽  
Amino Acid ◽  
Metal Ions ◽  
Paper Chromatography ◽  
Amino Acid Analysis ◽  
Heavy Metal Ions ◽  
High Pressures ◽  
Biological Fluids

An apparatus for the preparation of protein-free filtrates of biological fluids for subsequent amino-acid analysis by paper chromatography is described. The construction allows filtration to be carried out at high pressures, hence increasing the rate of filtration. Contamination by interfering heavy metal ions is avoided by a design in which solutions are exposed only to lucite.

Purification of Plant Amino Acid for Paper Chromatography

1959 ◽  
Vol 31 (6) ◽  
pp. 1028-1031 ◽  
Author(s):  
J. F. Thompson ◽  
C. J. Morris ◽  
R. K. Gering
Keyword(s):  
Amino Acid ◽  
Paper Chromatography

AMINO ACID COMPOSITION OF SOME INDIAN VEGETABLES AS DETERMINED BY PAPER CHROMATOGRAPHY

1956 ◽  
Vol 21 (4) ◽  
pp. 477-480 ◽  
Author(s):  
S. GHOSH MAJUMDER ◽  
RABINDRA NATH DUTTA ◽  
N. C. GANGULI
Keyword(s):  
Amino Acid ◽  
Amino Acid Composition ◽  
Acid Composition ◽  
Paper Chromatography

scholarly journals Studies on Monotreme Proteins II. Amino Acid Sequence of the a-Chain in Haemoglobin From the Echidna, Tachyglossus Aculeatus Aculeatus

1973 ◽  
Vol 26 (4) ◽  
pp. 877 ◽  
Author(s):  
RG Whittaker ◽  
WK Fisher ◽  
EOP Thompson
Keyword(s):  
Amino Acid ◽  
Amino Acid Sequence ◽  
Paper Chromatography ◽  
Gel Filtration ◽  
Tryptic Digestion ◽  
A Chain ◽  
Tachyglossus Aculeatus

The amino acid sequence of the 141 residues of the IX-chain of the major haemoglobin (Hb-IB) from the echidna has been determined. The soluble peptides formed by tryptic digestion were isolated by gel filtration, paper ionophoresis, and paper chromatography.

scholarly journals Haemoglobins of the Shark, Heterodontus Portusjacksoni II. Amino Acid Sequence of the a-Chain

1976 ◽  
Vol 29 (2) ◽  
pp. 73 ◽  
Author(s):  
AR Nash ◽  
WK Fisher ◽  
EOP Thompson
Keyword(s):  
Amino Acid ◽  
Amino Acid Sequence ◽  
Paper Chromatography ◽  
Cyanogen Bromide ◽  
Gel Filtration ◽  
Amino Acid Sequences ◽  
Tryptic Digestion ◽  
Amino Terminal ◽  
Core Peptide ◽  
A Chain

The amino acid sequence of the a-chain of the principal haemoglobin from the shark, H. portusjacksoni has been determined. The chain has 148 residues and is acetylated at the amino terminal. The soluble peptides obtained by tryptic and chymotryptic digestion of the protein or its cyanogen bromide fragments were isolated by gel filtration, paper ionophoresis and paper chromatography. The amino acid sequences were determined by the dansyl-Edman procedure. The insoluble 'core' peptide from the tryptic digestion contained 34 residues and required cleavage by several proteases before the sequence was established. Compared with human a-chain there are 88 amino acid differences including the additional seven residues which appear on the amino terminal of the shark chain. There is also one deletion and one insertion. The chain contains no tryptophan but has four cysteinyl residues which is the highest number of such residues recorded for a vertebrate globin.

Investigation of subtilisin digest of pepsin chain between half-cystines II and III

1981 ◽  
Vol 46 (3) ◽  
pp. 640-654
Author(s):  
Vladimír Kostka
Keyword(s):  
Amino Acid ◽  
Paper Chromatography ◽  
Sequential Analysis ◽  
Cysteine Residue ◽  
Amino Acid Sequences ◽  
Tryptic Digestion ◽  
Hydrolysis Of ◽  
Dowex 1 ◽  
Final Purification

Aminoethylated hog pepsin was subjected to tryptic digestion and the longest fragment, arising from cleavage at S-(β-aminoethyl)-cysteine residue No II and III was isolated from the digest. This fragment was subjected to additional cleavage with subtilisin and the digest resolved into crude fractions by chromatography on Dowex 1. The isolation and final purification of the peptides was carried out by paper electrophoreses and paper chromatography. By these methods 55 peptides were obtained which were subjected to sequential analysis by stepwise degradation. The amino acid sequences of these peptides and their position in the pepsin chain are given. These sequences provided overlaps for peptides obtained by hydrolysis of this part of the pepsin chain by other enzymes.

A Rapid and Simple Thin-Layer Chromatographic Method for Amino Acids in Blood

1969 ◽  
Vol 15 (9) ◽  
pp. 902-907 ◽  
Author(s):  
William J Culley
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Thin Layer ◽  
Paper Chromatography ◽  
Amino Acid Metabolism ◽  
Chromatographic Method ◽  
Acid Metabolism ◽  
Inborn Errors ◽  
Chromatographic Methods ◽  
Finger Tip

Abstract Simple and rapid thin-layer chromatographic methods, using heel or finger-tip blood, are described that are suitable for screening for inborn errors of amino acid metabolism. Serum is chromatographed without deproteinizing. A variation of this method utilizes blood impregnated on paper discs for chromatography. These methods are much faster, more sensitive, and provide better resolution of amino acids than paper chromatography. Because untreated serum- or blood-impregnated discs are placed directly on thin-layer chromatograms, these methods require considerably less working time than other thin-layer chromatographic methods.

The Amino Acid Compositions of Several Wool Fractions as Determined by Paper Chromatography

1955 ◽  
Vol 25 (4) ◽  
pp. 334-342 ◽  
Author(s):  
R.L. Golden ◽  
J.C. Whitwell ◽  
E.H. Mercer
Keyword(s):  
Amino Acid ◽  
Paper Chromatography ◽  
Amino Acid Compositions
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