Relative Expression Analysis of Target Genes by Using Reverse Transcription-Quantitative PCR

2019 ◽  
pp. 51-63
Author(s):  
Rocío Liliana Gómez ◽  
Lorena Noelia Sendín
Keyword(s):  
Quantitative Pcr ◽  
Expression Analysis ◽  
Reverse Transcription ◽  
Target Genes ◽  
Relative Expression ◽  
Reverse Transcription Quantitative Pcr

scholarly journals Reliable Gene Expression Analysis by Reverse Transcription-Quantitative PCR: Reporting and Minimizing the Uncertainty in Data Accuracy

2014 ◽  
Vol 26 (10) ◽  
pp. 3829-3837 ◽  
Author(s):  
Tony Remans ◽  
Els Keunen ◽  
Geert Jan Bex ◽  
Karen Smeets ◽  
Jaco Vangronsveld ◽  
...  
Keyword(s):  
Gene Expression ◽  
Quantitative Pcr ◽  
Expression Analysis ◽  
Reverse Transcription ◽  
Gene Expression Analysis ◽  
Data Accuracy ◽  
Reverse Transcription Quantitative Pcr

scholarly journals Quantitative Expression Analysis in Brassica napus by Northern Blot Analysis and Reverse Transcription-Quantitative PCR in a Complex Experimental Setting

PLoS ONE ◽  
2016 ◽  
Vol 11 (9) ◽  
pp. e0163679 ◽  
Author(s):  
Annekathrin Rumlow ◽  
Els Keunen ◽  
Jan Klein ◽  
Philip Pallmann ◽  
Anja Riemenschneider ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Quantitative Pcr ◽  
Expression Analysis ◽  
Reverse Transcription ◽  
Northern Blot ◽  
Blot Analysis ◽  
Northern Blot Analysis ◽  
Experimental Setting ◽  
Quantitative Expression ◽  
Reverse Transcription Quantitative Pcr

scholarly journals Establishment and Application of Rapid Diagnosis for Reverse Transcription-Quantitative PCR of Newly Emerging Goose-Origin Nephrotic Astrovirus in China

mSphere ◽  
2018 ◽  
Vol 3 (6) ◽  
Author(s):  
Xiaoyuan Yuan ◽  
Kai Meng ◽  
Yuxia Zhang ◽  
Lihong Qi ◽  
Wu Ai ◽  
...  
Keyword(s):  
Quantitative Pcr ◽  
Clinical Application ◽  
Reverse Transcription ◽  
Test Sample ◽  
Sensitivity Testing ◽  
Content Type ◽  
Reverse Transcription Quantitative Pcr ◽  
Emerging Virus ◽  
New Type ◽  
And Control

ABSTRACT In 2017, a new type of goose-origin astrovirus (GoAstV) that is completely different from previously identified avian astroviruses (which have only 30.0% to 50.5% homology with GoAstV) has been isolated from diseased geese in China. This disease can cause joint swelling in sick geese, and the anatomy shows a clear precipitation of urate in the kidney. The rate of death and culling can reach more than 30%, revealing the disease’s severe pathogenicity. To quickly and accurately diagnose the newly emerging disease, we established a highly specific reverse transcription-quantitative PCR (RT-qPCR) method of detecting GoAstV. Sensitivity testing showed that the minimum amount of test sample for this method is 52.5 copies/μl. Clinical application confirmed that this method can quickly and effectively detect GoAstV, providing a diagnostic platform for the prevention and control of goose disease. IMPORTANCE Goose-origin astrovirus (GoAstV), as a newly emerging virus in 2017, is different from previously known astroviruses in the genus Avastrovirus. So far, few studies have focused on the novel virus. Considering the infectious development of astrovirus (AstV), we established a reverse transcription-quantitative PCR (RT-qPCR) assay with a strong specificity to quickly and accurately diagnose GoAstV. Confirmed by clinical application, this method can quickly and accurately detect prevalent GoAstV. The assay is thus convenient for clinical operation and is applicable to the monitoring of GoAstV disease.

scholarly journals Selection of Valid Reference Genes for Reverse Transcription Quantitative PCR Analysis inHeliconius numata(Lepidoptera: Nymphalidae)

2016 ◽  
Vol 16 (1) ◽  
pp. 50 ◽  
Author(s):  
Florence Piron Prunier ◽  
Mathieu Chouteau ◽  
Annabel Whibley ◽  
Mathieu Joron ◽  
Violaine Llaurens
Keyword(s):  
Quantitative Pcr ◽  
Reverse Transcription ◽  
Reference Genes ◽  
Pcr Analysis ◽  
Reverse Transcription Quantitative Pcr ◽  
Selection Of

scholarly journals Diversity of Intestinal Clostridium coccoides Group in the Japanese Population, as Demonstrated by Reverse Transcription-Quantitative PCR

PLoS ONE ◽  
2015 ◽  
Vol 10 (5) ◽  
pp. e0126226 ◽  
Author(s):  
Takashi Kurakawa ◽  
Kiyohito Ogata ◽  
Kazunori Matsuda ◽  
Hirokazu Tsuji ◽  
Hiroyuki Kubota ◽  
...  
Keyword(s):  
Quantitative Pcr ◽  
Reverse Transcription ◽  
Japanese Population ◽  
Reverse Transcription Quantitative Pcr
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