scholarly journals Continuous and Real-Time In Vivo Autobioluminescent Imaging in a Mouse Model

2019 ◽  
pp. 191-201
Author(s):  
Derek Yip ◽  
Andrew Kirkpatrick ◽  
Tingting Xu ◽  
Tom Masi ◽  
Stacy Stephenson ◽  
...  
Keyword(s):  
Mouse Model ◽  
Real Time

scholarly journals Real-time in vivo imaging of regional lung function in a mouse model of cystic fibrosis on a laboratory X-ray source

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Rhiannon P. Murrie ◽  
Freda Werdiger ◽  
Martin Donnelley ◽  
Yu-wei Lin ◽  
Richard P. Carnibella ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Lung Function ◽  
Mouse Model ◽  
Real Time ◽  
In Vivo Imaging ◽  
X Ray ◽  
Regional Lung Function ◽  
Regional Lung

scholarly journals Visualizing ATP Dynamics in Live Mice

2020 ◽  
Author(s):  
Norimichi Koitabashi ◽  
Riki Ogasawara ◽  
Ryuto Yasui ◽  
Yuki Sugiura ◽  
Hinako Matsuda ◽  
...  
Keyword(s):  
Mouse Model ◽  
Real Time ◽  
Biological Activities ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Spatiotemporal Resolution ◽  
Physiological Mechanisms ◽  
Multiple Organs ◽  
Atp Levels

ABSTRACTAnalysis of the dynamics of adenosine triphosphate (ATP) is vital to quantitatively define the actual roles of ATP in biological activities. Here, we applied a genetically encoded Förster resonance energy transfer biosensor “GO-ATeam” and created a transgenic mouse model that allows systemic ATP levels to be quantitatively, sensitively, noninvasively, and spatiotemporally measured under physiological and pathological conditions. We used this model to readily conduct intravital imaging of ATP dynamics under three different conditions: during exercise, in all organs and cells; during myocardial infarction progression; and in response to the application of cardiotoxic drugs. These findings provide compelling evidence that the GO-ATeam mouse model is a powerful tool to investigate the multifarious functions of cellular ATP in vivo with unprecedented spatiotemporal resolution in real-time. This will inform predictions of molecular and morphological responses to perturbations of ATP levels, as well as the elucidation of physiological mechanisms that control ATP homeostasis.One Sentence SummaryIntravital real-time imaging of ATP dynamics in multiple organs using GO-ATeam mice, can be used to quantitatively, sensitively, noninvasively, and spatiotemporally measure systemic ATP levels and provide a platform for preclinical pharmacological studies.

scholarly journals In Vivo Optical Imaging of Myelination Events in a Myelin Basic Protein Promoter-Driven Luciferase Transgenic Mouse Model

ASN NEURO ◽  
2018 ◽  
Vol 10 ◽  
pp. 175909141877732 ◽  
Author(s):  
James Cao ◽  
Yanping Hu ◽  
Mohammed Salman Shazeeb ◽  
Carlos E. Pedraza ◽  
Nilesh Pande ◽  
...  
Keyword(s):  
Mouse Model ◽  
Myelin Basic Protein ◽  
Real Time ◽  
Transgenic Mouse ◽  
Basic Protein ◽  
Neuronal Cell ◽  
Transgenic Mouse Model ◽  
Luciferase Reporter ◽  
Transcriptional Level

The compact myelin sheath is important for axonal function, and its loss can lead to neuronal cell death and irreversible functional deficits. Myelin is vulnerable to a variety of metabolic, toxic, and autoimmune insults. In diseases like multiple sclerosis, there is currently no therapy to stop myelin loss, underscoring the need for neuroprotective and remyelinating therapies. Noninvasive, robust techniques are also needed to confirm the effect of such therapies in animal models. This article describes the generation, characterization, and potential uses for a myelin basic protein-luciferase (MBP-luci) transgenic mouse model, in which the firefly luciferase reporter gene is selectively controlled by the MBP promoter. In vivo bioluminescence imaging can be used to visualize and quantify demyelination and remyelination at the transcriptional level, noninvasively, and in real time. Transgenic mice were assessed in the cuprizone-induced model of demyelination, and luciferase activity highly correlated with demyelination and remyelination events as confirmed by both magnetic resonance imaging and postmortem histological analysis. Furthermore, MBP-luci mice demonstrated enhanced luciferase signal and remyelination in the cuprizone model after treatment with a peroxisome proliferator activated receptor-delta selective agonist and quetiapine. Imaging sensitivity was further enhanced by using CycLuc 1, a luciferase substrate, which has greater blood–brain barrier penetration. We demonstrated the utility of MBP-luci model in tracking myelin changes in real time and supporting target and therapeutic validation efforts.

scholarly journals Rapid Direct Method for Monitoring Antibiotics in a Mouse Model of Bacterial Biofilm Infection

2003 ◽  
Vol 47 (10) ◽  
pp. 3130-3137 ◽  
Author(s):  
Jagath L. Kadurugamuwa ◽  
Lin V. Sin ◽  
Jun Yu ◽  
Kevin P. Francis ◽  
Richard Kimura ◽  
...  
Keyword(s):  
Mouse Model ◽  
Real Time ◽  
Direct Method ◽  
Bacterial Biofilm ◽  
Dependent Manner ◽  
Chronic Infections ◽  
Biophotonic Imaging ◽  
Biofilm Infection

ABSTRACT We have developed a rapid, continuous method for monitoring the effectiveness of several antibacterial agents in real time, noninvasively, by using a recently described mouse model of chronic biofilm infection (J. L. Kadurugamuwa et al., Infect. Immun. 71:882-890, 2003), which relies on biophotonic imaging of bioluminescent bacteria. To facilitate real-time monitoring of infection, we used a Staphylococcus aureus isolate that was made bioluminescent by inserting a modified lux operon into the bacterial chromosome. This bioluminescent reporter bacterium was used to study the antimicrobial effects of several antibiotics belonging to different molecular families. Treatment with rifampin, tobramycin, and ciprofloxacin was started 7 days after subcutaneous implantation of catheters precolonized with 104 CFU of S. aureus. Three different doses of antibiotics were administered twice a day for 4 consecutive days. The number of metabolically active bacteria in untreated mice and the tobramycin- and ciprofloxacin-treated groups remained relatively unchanged over the 4-week observation period, indicating poor efficacies for tobramycin and ciprofloxacin. A rapid dose-dependent decline in metabolic activity in rifampin-treated groups was observed, with almost a 90% reduction after two doses and nearly undetectable levels after three doses. The disappearance of light emission correlated with colony counts. After the final treatment, cell numbers rebounded as a function of concentration in a time-dependent manner. The staphylococci isolated from the catheters of mice treated with rifampin were uniformly resistant to rifampin but retained their in vitro susceptibilities to tobramycin and ciprofloxacin. Since the metabolic activities of viable cells and a postantibiotic effect could be detected directly on the support matrix nondestructively and noninvasively, the methodology is specifically appealing for investigating the effects of antibiotics on biofilms in vivo. Moreover, our study points to the possible use of biophotonic imaging for the detection of the development of resistance to therapeutic agents during treatment of chronic infections in vivo.

scholarly journals Optical nanosensors for in vivo physiological chloride detection for monitoring cystic fibrosis treatment

2020 ◽  
Vol 12 (11) ◽  
pp. 1441-1448 ◽  
Author(s):  
Wenjun Di ◽  
Heather A. Clark
Keyword(s):  
Cystic Fibrosis ◽  
Mouse Model ◽  
Real Time ◽  
Pharmacological Treatment ◽  
Cystic Fibrosis Mouse ◽  
Optical Nanosensors

Endogenous chloride fluctuations were successfully monitored in real-time using optode-based nanosensors during pharmacological treatment in a cystic fibrosis mouse model.

Monitoring of tumor burden in vivo by optical imaging in a xenograft SCID mouse model: evaluation of two fluorescent proteins of the GFP-superfamily

2018 ◽  
Vol 60 (3) ◽  
pp. 315-326 ◽  
Author(s):  
Ingrid Böhm ◽  
Stephan Gehrke ◽  
Beate Kleb ◽  
Martin Hungerbühler ◽  
Rolf Müller ◽  
...  
Keyword(s):  
Mouse Model ◽  
Real Time ◽  
Fluorescent Protein ◽  
Fluorescent Proteins ◽  
Severe Combined Immunodeficiency ◽  
Liver Parenchyma ◽  
Human Malignant Melanoma ◽  
Signal Intensities

Background Mouse models of human-malignant-melanoma (MM) are important tools to study tumor dynamics. The enhanced green fluorescent protein (EGFP) is widely used in molecular imaging approaches, together with optical scanners, and fluorescence imaging. Purpose Currently, there are no data available as to whether other fluorescent proteins are more suitable. The goal of this preclinical study was to analyze two fluorescent proteins of the GFP superfamily under real-time in vivo conditions using fluorescence reflectance imaging (FRI). Material and Methods The human melanoma cell line MeWo was stable transfected with one plasmid: pEGFP-C1 or pDsRed1-N1. We investigated two severe combined immunodeficiency (SCID)-mice groups: A (solid xenografts) and B (xenografts as metastases). After three weeks, the animals were weekly imaged by FRI. Afterwards the mice were euthanized and metastases were imaged in situ: to quantify the cutis-dependent reduction of emitted light, we compared signal intensities obtained by metastases in vivo with signal intensities obtained by in situ liver parenchyma preparations. Results More than 90% of cells were stable transfected. EGFP-/DsRed-xenograft tumors had identical growth kinetics. In vivo the emitted light by DsRed tumors/metastases was much brighter than by EGFP. DsRed metastases were earlier (3 vs. 5 weeks) and much more sensitive detectable than EGFP metastases. Cutis-dependent reduction of emitted light was greater in EGFP than in DsRed mice (tenfold). Autofluorescence of DsRed was lower than of EGFP. Conclusion We established an in vivo xenograft mouse model (DsRed-MeWo) that is reliable, reproducible, and superior to the EGFP model as a preclinical tool to study innovative therapies by FRI under real-time in vivo conditions.

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