Bioluminescent Imaging and Tracking of Bacterial Transport in Soils

2019 ◽  
pp. 53-65
Author(s):  
Jie Zhuang ◽  
Weipeng Liu ◽  
Liqiong Yang ◽  
Jia Kang ◽  
Xiaoming Zhang
Keyword(s):  
Bacterial Transport ◽  
Bioluminescent Imaging

scholarly journals Rates of ligand binding to periplasmic proteins involved in bacterial transport and chemotaxis.

1983 ◽  
Vol 258 (22) ◽  
pp. 13665-13672 ◽  
Author(s):  
D M Miller ◽  
J S Olson ◽  
J W Pflugrath ◽  
F A Quiocho
Keyword(s):  
Ligand Binding ◽  
Bacterial Transport ◽  
Periplasmic Proteins

scholarly journals Real-Time In Vivo Bioluminescent Imaging for Evaluating the Efficacy of Antibiotics in a Rat Staphylococcus aureus Endocarditis Model

2005 ◽  
Vol 49 (1) ◽  
pp. 380-387 ◽  
Author(s):  
Yan Q. Xiong ◽  
Julie Willard ◽  
Jagath L. Kadurugamuwa ◽  
Jun Yu ◽  
Kevin P. Francis ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Real Time ◽  
Imaging System ◽  
Bioluminescent Imaging ◽  
Vancomycin Therapy ◽  
Treatment Groups ◽  
Highly Sensitive ◽  
Relevant Animal Model ◽  
And Control

ABSTRACT Therapeutic options for invasive Staphylococcus aureus infections have become limited due to rising antimicrobial resistance, making relevant animal model testing of new candidate agents more crucial than ever. In the present studies, a rat model of aortic infective endocarditis (IE) caused by a bioluminescently engineered, biofilm-positive S. aureus strain was used to evaluate real-time antibiotic efficacy directly. This strain was vancomycin and cefazolin susceptible but gentamicin resistant. Bioluminescence was detected and quantified daily in antibiotic-treated and control animals with IE, using a highly sensitive in vivo imaging system (IVIS). Persistent and increasing cardiac bioluminescent signals (BLS) were observed in untreated animals. Three days of vancomycin therapy caused significant reductions in both cardiac BLS (>10-fold versus control) and S. aureus densities in cardiac vegetations (P < 0.005 versus control). However, 3 days after discontinuation of vancomycin therapy, a greater than threefold increase in cardiac BLS was observed, indicating relapsing IE (which was confirmed by quantitative culture). Cefazolin resulted in modest decreases in cardiac BLS and bacterial densities. These microbiologic and cardiac BLS differences during therapy correlated with a longer time-above-MIC for vancomycin (>12 h) than for cefazolin (∼4 h). Gentamicin caused neither a reduction in cardiac S. aureus densities nor a reduction in BLS. There were significant correlations between cardiac BLS and S. aureus densities in vegetations in all treatment groups. These data suggest that bioluminescent imaging provides a substantial advance in the real-time monitoring of the efficacy of therapy of invasive S. aureus infections in live animals.

scholarly journals Tracking Early Autoimmune Disease by Bioluminescent Imaging of NF-κB Activation Reveals Pathology in Multiple Organ Systems

2009 ◽  
Vol 174 (4) ◽  
pp. 1358-1367 ◽  
Author(s):  
Michael Zangani ◽  
Harald Carlsen ◽  
Anders Kielland ◽  
Audun Os ◽  
Harald Hauglin ◽  
...  
Keyword(s):  
Autoimmune Disease ◽  
Multiple Organ ◽  
Bioluminescent Imaging ◽  
Organ Systems

Use of non-invasive bioluminescent imaging to assess mycobacterial dissemination in mice, treatment with bactericidal drugs and protective immunity

2009 ◽  
Vol 11 (14-15) ◽  
pp. 1114-1121 ◽  
Author(s):  
Frank Heuts ◽  
Berit Carow ◽  
Hans Wigzell ◽  
Martin E. Rottenberg
Keyword(s):  
Protective Immunity ◽  
Bioluminescent Imaging ◽  
Non Invasive

scholarly journals Noninvasive Bioluminescent Imaging Demonstrates Long-Term Multilineage Engraftment of Ex Vivo-Expanded CD34-Selected Umbilical Cord Blood Cells

Stem Cells ◽  
2009 ◽  
Vol 27 (8) ◽  
pp. 1932-1940 ◽  
Author(s):  
David Steiner ◽  
Juri Gelovani ◽  
Barbara Savoldo ◽  
Simon N. Robinson ◽  
William K. Decker ◽  
...  
Keyword(s):  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Blood Cells ◽  
Ex Vivo ◽  
Bioluminescent Imaging ◽  
Cord Blood Cells ◽  
Umbilical Cord Blood Cells

scholarly journals Bioluminescent Imaging of Pneumococcal Otitis Media in Chinchillas

2013 ◽  
Vol 122 (5) ◽  
pp. 344-352 ◽  
Author(s):  
Alan W. Johnson ◽  
James D. Sidman ◽  
Jizhen Lin
Keyword(s):  
Otitis Media ◽  
Bioluminescent Imaging

scholarly journals In vivo monitoring of fetoplacental Vegfr2 gene activity in a murine pregnancy model using a Vegfr2-luc reporter gene and bioluminescent imaging

2011 ◽  
Vol 9 (1) ◽  
pp. 51 ◽  
Author(s):  
Jonathan M Greene ◽  
Chad W Dunaway ◽  
Susan D Bowers ◽  
Brian J Rude ◽  
Jean M Feugang ◽  
...  
Keyword(s):  
Reporter Gene ◽  
Gene Activity ◽  
Bioluminescent Imaging ◽  
In Vivo Monitoring ◽  
Luc Reporter Gene ◽  
Murine Pregnancy

scholarly journals Lessons Learned from Bacterial Transport Research at the South Oyster Site

Ground Water ◽  
2011 ◽  
Vol 49 (5) ◽  
pp. 745-763 ◽  
Author(s):  
Timothy D. Scheibe ◽  
Susan S. Hubbard ◽  
Tullis C. Onstott ◽  
Mary F. DeFlaun
Keyword(s):  
Lessons Learned ◽  
Bacterial Transport ◽  
The South
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