Immuno-Laser Capture Microdissection of Rat Brain Neurons for Real Time Quantitative PCR

2009 ◽  
pp. 219-230 ◽  
Author(s):  
Denis G. Baskin ◽  
L. Scot Bastian
Keyword(s):  
Rat Brain ◽  
Real Time ◽  
Quantitative Pcr ◽  
Laser Capture Microdissection ◽  
Brain Neurons ◽  
Real Time Quantitative Pcr ◽  
Rat Brain Neurons ◽  
Laser Capture

scholarly journals Immunocytochemistry and Laser Capture Microdissection for Real-time Quantitative PCR Identify Hindbrain Neurons Activated by Interaction Between Leptin and Cholecystokinin

2007 ◽  
Vol 56 (3) ◽  
pp. 285-293 ◽  
Author(s):  
Diana L. Williams ◽  
Michael W. Schwartz ◽  
L. Scot Bastian ◽  
James E. Blevins ◽  
Denis G. Baskin
Keyword(s):  
Real Time ◽  
Quantitative Pcr ◽  
Laser Capture Microdissection ◽  
Neuronal Response ◽  
Current Evidence ◽  
Real Time Quantitative Pcr ◽  
Th Cell ◽  
Mrna Content ◽  
Catecholamine Neurons ◽  
Laser Capture

Current evidence suggests that leptin reduces food intake in part by enhancing the hindbrain neuronal response to meal-related gastrointestinal signals, including cholecystokinin (CCK), but the phenotypes of the relevant cells are not known. To identify neurons that participate in this interaction in the rat nucleus of the solitary tract (NTS), we induced c-Fos gene expression in NTS neurons with leptin and CCK. We focused on NTS catecholamine neurons because these cells have been implicated in the feeding response to CCK. Hindbrain sections from rats that received CCK with or without leptin pretreatment were immunostained for c-Fos and tyrosine hydroxylase (TH) by a double immunofluorescence procedure. Leptin pretreatment increased the number of NTS cells expressing c-Fos-like immunoreactivity (cFLI) 3-fold relative to CCK alone, but the number of TH-positive cells with cFLI was increased 6-fold. Next, cells detected by immunofluorescence for TH were collected by laser capture microdissection and pooled for real-time quantitative PCR of c-Fos mRNA. Here, neither leptin nor CCK alone affected the relative amount of mRNA in the TH cell–enriched samples, but leptin plus CCK substantially increased c-Fos mRNA content. These histochemical findings identify hindbrain catecholamine cells as potential mediators of the interaction between leptin and CCK.

Distribution and MK-801-induced expression of serine racemase mRNA in rat brain by real-time quantitative PCR

2004 ◽  
Vol 128 (1) ◽  
pp. 90-94 ◽  
Author(s):  
Masanobu Yoshikawa ◽  
Tomomi Kobayashi ◽  
Tetsuo Oka ◽  
Mitsuru Kawaguchi ◽  
Atsushi Hashimoto
Keyword(s):  
Rat Brain ◽  
Real Time ◽  
Quantitative Pcr ◽  
Serine Racemase ◽  
Induced Expression ◽  
Mk 801 ◽  
Real Time Quantitative Pcr

scholarly journals Quantification and Stability of Human Adenoviruses and Polyomavirus JCPyV in Wastewater Matrices

2006 ◽  
Vol 72 (12) ◽  
pp. 7894-7896 ◽  
Author(s):  
Silvia Bofill-Mas ◽  
Nestor Albinana-Gimenez ◽  
Pilar Clemente-Casares ◽  
Ayalkibet Hundesa ◽  
Jesus Rodriguez-Manzano ◽  
...  
Keyword(s):  
Real Time ◽  
Quantitative Pcr ◽  
High Stability ◽  
Wastewater Sludge ◽  
Human Polyomavirus ◽  
Sewage Effluent ◽  
Viral Contamination ◽  
Human Adenoviruses ◽  
Urban Sewage ◽  
Real Time Quantitative Pcr

ABSTRACT Human adenoviruses (HAdV) and human polyomavirus JCPyV have been previously proposed as indicators of fecal viral contamination in the environment. Different wastewater matrices have been analyzed by applying real-time quantitative PCR procedures for the presence, quantity, and stability of a wide diversity of excreted HAdV and JCPyV. High quantities of HAdV and JCPyV were detected in sewage, effluent wastewater, sludge, and biosolid samples. Both viruses showed high stability in urban sewage. These results confirm the suitability of both viruses as indicators of human fecal viral pollution.

Analysis of Caecal Microbiota in Rats Fed with Genetically Modified Rice by Real-Time Quantitative PCR

2011 ◽  
Vol 76 (1) ◽  
pp. M88-M93 ◽  
Author(s):  
Wentao Xu ◽  
Liting Li ◽  
Jiao Lu ◽  
YunBo Luo ◽  
Ying Shang ◽  
...  
Keyword(s):  
Real Time ◽  
Quantitative Pcr ◽  
Genetically Modified ◽  
Genetically Modified Rice ◽  
Real Time Quantitative Pcr ◽  
Caecal Microbiota
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