Quantitative Analysis of Oxidized Guanine, 8-Oxoguanine, in Mitochondrial DNA by Immunofluorescence Method

2009 ◽  
pp. 199-212 ◽  
Author(s):  
Mizuki Ohno ◽  
Sugako Oka ◽  
Yusaku Nakabeppu
Keyword(s):  
Mitochondrial Dna ◽  
Quantitative Analysis ◽  
Immunofluorescence Method ◽  
Oxidized Guanine

scholarly journals THE METHOD OF MOLECULAR AND GENETIC DIAGNOSIS OF THE PROCESS OF CELL SENESCENCE BASED ON QUANTITATIVE ANALYSIS OF NUCLEAR AND MITOCHONDRIAL DNA GENES

2016 ◽  
pp. 46-50
Author(s):  
E. V. Voropayev ◽  
A. A. Ziatskov ◽  
O. V. Osipkina ◽  
O. Y. Baranov ◽  
N. V. Galinovskaya ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Quantitative Analysis ◽  
Genetic Diagnosis ◽  
Cell Senescence

scholarly journals Quantitative analysis of mitochondrial DNA deletions in the brains of patients with bipolar disorder and schizophrenia

2005 ◽  
Vol 8 (04) ◽  
pp. 515 ◽  
Author(s):  
Chihiro Kakiuchi ◽  
Mizuho Ishiwata ◽  
Mizue Kametani ◽  
Cheryl Nelson ◽  
Kazuya Iwamoto ◽  
...  
Keyword(s):  
Bipolar Disorder ◽  
Mitochondrial Dna ◽  
Quantitative Analysis ◽  
Dna Deletions

Quantitative analysis of leukocyte mitochondrial DNA deletion in affective disorders

1997 ◽  
Vol 42 (5) ◽  
pp. 311-316 ◽  
Author(s):  
Tadafumi Kato ◽  
George Winokur ◽  
Francis J. McMahon ◽  
J. Raymond DePaulo ◽  
Raymond R. Crowe
Keyword(s):  
Mitochondrial Dna ◽  
Quantitative Analysis ◽  
Affective Disorders ◽  
Mitochondrial Dna Deletion ◽  
Dna Deletion

scholarly journals Sirt3 protects mitochondrial DNA damage and blocks the development of doxorubicin-induced cardiomyopathy in mice

2016 ◽  
Vol 310 (8) ◽  
pp. H962-H972 ◽  
Author(s):  
Vinodkumar B. Pillai ◽  
Samik Bindu ◽  
Will Sharp ◽  
Yong Hu Fang ◽  
Gene Kim ◽  
...  
Keyword(s):  
Dna Damage ◽  
Mitochondrial Dna ◽  
Primary Cultures ◽  
Dna Glycosylase ◽  
Drug Induced ◽  
Mtdna Damage ◽  
Mitochondrial Dna Damage ◽  
Delayed Toxicity ◽  
Oxidized Guanine ◽  
Dose Dependent

Doxorubicin (Doxo) is a chemotherapeutic drug widely used to treat variety of cancers. One of the most serious side effects of Doxo is its dose-dependent and delayed toxicity to the heart. Doxo is known to induce cardiac mitochondrial damage. Recently, the mitochondrial sirtuin SIRT3 has been shown to protect mitochondria from oxidative stress. Here we show that overexpression of SIRT3 protects the heart from toxicity of Doxo by preventing the drug-induced mitochondrial DNA (mtDNA) damage. Doxo treatment caused depletion of Sirt3 levels both in primary cultures of cardiomyocytes and in mouse hearts, which led to massive acetylation of mitochondrial proteins. Doxo-induced toxicity to cardiomyocytes was associated with increased reactive oxygen species (ROS) production, mitochondrial fragmentation, and cell death. Overexpression of SIRT3 helped to attenuate Doxo-induced ROS levels and cardiomyocyte death. Sirt3 knockout (Sirt3.KO) mice could not endure the full dose of Doxo treatment, developed exacerbated cardiac hypertrophy, and died during the course of treatment, whereas Sirt3 transgenic (Sirt3.tg) mice were protected against Doxo-induced cardiotoxicity. Along with Sirt3, we also observed a concomitant decrease in levels of oxoguanine-DNA glycosylase-1 (OGG1), a major DNA glycosylase that hydrolyzes oxidized-guanine (8-oxo-dG) to guanine. Depletion of OGG1 levels was associated with increased mtDNA damage. Sirt3.KO mice and Doxo-treated mice showed increased 8-oxo-dG adducts in DNA and corresponding increase in mtDNA damage, whereas, 8-oxo-dG adducts and mtDNA damage were markedly reduced in Sirt3 overexpressing transgenic mice hearts. These results thus demonstrated that Sirt3 activation protects the heart from Doxo-induced cardiotoxicity by maintaining OGG1 levels and protecting mitochondria from DNA damage.

scholarly journals Quantitative analysis of mitochondrial DNA 4977-bp deletion in sporadic breast cancer and benign breast diseases

2007 ◽  
Vol 108 (3) ◽  
pp. 427-434 ◽  
Author(s):  
Chuanzhong Ye ◽  
Xiao-Ou Shu ◽  
Wanqing Wen ◽  
Larry Pierce ◽  
Regina Courtney ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Mitochondrial Dna ◽  
Quantitative Analysis ◽  
Sporadic Breast Cancer ◽  
Breast Diseases ◽  
Benign Breast Diseases

Quantitative Analysis of Somatic Mitochondrial DNA Mutations by Single-Cell Single-Molecule PCR

2009 ◽  
pp. 329-369 ◽  
Author(s):  
Yevgenya Kraytsberg ◽  
Natalya Bodyak ◽  
Susan Myerow ◽  
Alexander Nicholas ◽  
Konstantin Ebralidze ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Quantitative Analysis ◽  
Single Cell ◽  
Single Molecule ◽  
Mitochondrial Dna Mutations ◽  
Dna Mutations
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