Detection of Proteins and Sialoglycoproteins in Polyacrylamide Gels Using Eosin Y Stain

SummaryThe presence of soluble fibrin complexes (SFC) measured by gel filtration of plasma on 4% agarose columns, fibrinogen heterogeneity on 3.5% SDS-polyacrylamide gels and the concentrations of several plasma proteins were evaluated in 39 patients with diabetes mellitus (DM) and 19 matched control subjects. A small but significant increase of SFC was found in DM (p<0.01). On individual basis 51.2% of the patients had increased SFC (>M + 2 SD of the controls). Polyacrylamide gel electrophoresis of the SFC showed no evidence of cross-linking or proteolysis. Plasma clots formed in the presence of EDTA and trasylol were analysed in SDS-polyacrylamide gels in a normal and two lower molecular weight fibrin bands (band I, II, III). The percentage of band I fibrinogen was in diabetics (65.3 ± 4.7%) lower than that of the controls (71.8 ± 4.5%) (p < 0.01). Fibrinogen levels, antithrombin III, α1-antitrypsin, α2-macroglobulin and plasminogen were significantly increased in DM. We suggest that in DM there is an enhancement of intravascular fibrin formation and accelerated fibrinogen degradation to lower molecular weight forms.

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A simple, rapid and high-resolution banding method in polyacrylamide gels

Hereditas (Beijing) ◽

10.3724/sp.j.1005.2008.00251 ◽

2008 ◽

Vol 30 (2) ◽

pp. 251-254 ◽

Cited By ~ 2

Author(s):

Yu-Shan ZHANG

Keyword(s):

High Resolution ◽

Polyacrylamide Gels ◽

Resolution Banding

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Rhodamines as Photocatalyst in Organic Synthesis

Current Green Chemistry ◽

10.2174/2213346107999201026201232 ◽

2020 ◽

Vol 07 ◽

Author(s):

Avik K. Bagdi ◽

Papiya Sikdar

Keyword(s):

Visible Light ◽

Organic Synthesis ◽

Organic Dyes ◽

Transition Metal Catalysts ◽

Visible Light Photocatalysis ◽

Eosin Y ◽

Organic Transformations ◽

The Sustainable Development ◽

Mechanistic Pathway ◽

Green Model

Abstract:: Organic synthesis under environment friendly conditions has great impact in the sustainable development. In this context, visible light photocatalysis has emerged as a green model as this offers an energy-efficient pathway towards the organic transformation. Different transition-metal catalysts (Ir-, Ru-, Cu- etc) and organic dyes (eosin Y, rose bengal, methylene blue etc) are well-known photocatalysts in organic synthesis. Apart from the well-known organophotoredox catalysts, rhodamines (Rhodamine B and Rhodamine 6G) have been also employed as efficient photocatalysts for different organic transformations. In this review, we will focus on the photocatalysis by rhodamines in organic synthesis. Mechanistic pathway of the methodologies will also be discussed. We believe this review will stimulate the employment of rhodamines in the visible light photocatalysis for efficient organic transformations in the future.

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Effect of agents affecting water structure on the uptake of saccharides by agar and polyacrylamide gels

Biopolymers ◽

10.1002/bip.1974.360131212 ◽

1974 ◽

Vol 13 (12) ◽

pp. 2553-2563 ◽

Cited By ~ 2

Author(s):

K. Sigler

Keyword(s):

Water Structure ◽

Polyacrylamide Gels

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Highly chemoselective deoxygenation of N-heterocyclic N-oxides under transition metal-free conditions

Organic & Biomolecular Chemistry ◽

10.1039/d1ob00260k ◽

2021 ◽

Vol 19 (16) ◽

pp. 3735-3742

Author(s):

Se Hyun Kim ◽

Ju Hyeon An ◽

Jun Hee Lee

Keyword(s):

Transition Metal ◽

Visible Light ◽

Eosin Y ◽

Metal Free ◽

Simple Protocol

Here, we provide an operationally simple protocol for the highly chemoselective deoxygenation of various functionalized N-heterocyclic N-oxides under visible light-mediated photoredox conditions with Na2-eosin Y as an organophotocatalyst.

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