Genome Size Determination Using Flow Cytometry of Propidium Iodide-Stained Nuclei

2011 ◽  
pp. 3-12 ◽  
Author(s):  
Emily E. Hare ◽  
J. Spencer Johnston
Keyword(s):  
Flow Cytometry ◽  
Genome Size ◽  
Propidium Iodide ◽  
Size Determination

scholarly journals Evolution of Genome Size in Duckweeds (Lemnaceae)

2011 ◽  
Vol 2011 ◽  
pp. 1-9 ◽  
Author(s):  
Wenqin Wang ◽  
Randall A. Kerstetter ◽  
Todd P. Michael
Keyword(s):  
Flow Cytometry ◽  
Genome Size ◽  
Propidium Iodide ◽  
Dna Content ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Continuous Increase ◽  
Spirodela Polyrhiza ◽  
Wolffia Arrhiza ◽  
Basic Reference

To extensively estimate the DNA content and to provide a basic reference for duckweed genome sequence research, the nuclear DNA content for 115 different accessions of 23 duckweed species was measured by flow cytometry (FCM) stained with propidium iodide as DNA stain. The 1C-value of DNA content in duckweed family varied nearly thirteen-fold, ranging from 150 megabases (Mbp) in Spirodela polyrhiza to 1,881 Mbp in Wolffia arrhiza. There is a continuous increase of DNA content in Spirodela, Landoltia, Lemna, Wolffiella, and Wolffia that parallels a morphological reduction in size. There is a significant intraspecific variation in the genus Lemna. However, no such variation was found in other studied species with multiple accessions of genera Spirodela, Landoltia, Wolffiella, and Wolffia.

scholarly journals Application of flow cytometry for genome size determination inGeosmithiafungi: A comparison of methods

2014 ◽  
Vol 85 (10) ◽  
pp. 854-861 ◽  
Author(s):  
Tereza Veselská ◽  
Jan Svoboda ◽  
Žaneta Růžičková ◽  
Miroslav Kolařík
Keyword(s):  
Flow Cytometry ◽  
Genome Size ◽  
Comparison Of Methods ◽  
Size Determination

scholarly journals Nuclear Genome Size Determination Of Christia Vespertilionis Via Flow Cytometry

2020 ◽  
Vol 4 (2) ◽  
pp. 72-75
Author(s):  
Mohd Razik Midin ◽  
Muhammad Irfan Fikri ◽  
Siti Sarah Zailani
Keyword(s):  
Flow Cytometry ◽  
Genome Size ◽  
Nuclear Genome ◽  
Size Determination ◽  
Size Estimation ◽  
Butterfly Wing ◽  
External Reference ◽  
Fcm Analysis ◽  
Suspension Preparation

AbstractChristia vespertilionis (butterfly wing plant) is an ornamental plant originated from South East Asia with reported usage in traditional medicine practice and potential as an anticancer and antitumor. This research aims to estimate the genome size of C. vespertilionis via flow cytometry (FCM) method. The research was conducted with the optimisation of nuclear suspension preparation followed by the genome size estimation. Two chopping techniques [manual chopping (MC) and BDTM Medimachine (MM)] and two lysis buffers (Otto and LBO1) were tested. Otto buffer with manual chopping was found to be the most suitable method, generated fine DNA peak with minimum debris background, and coefficient of variation (CV) value less than 3%. Five replicates of the FCM analysis were made for the genome size determination. The estimated genome size of C. vespertilionis was found to be 3.22 pg by using Glycine max cv. Polanka (2C=2.5pg) as an external reference standard. Further comparison with other Christia species was not possible due to the lack of data on genome size. The genome size data of C. vespertilionis can be useful for future morphology and genetics studies of Christia species.

GENOME SIZE DETERMINATION IN CHAGAS DISEASE TRANSMITTING BUGS (HEMIPTERA-TRIATOMINAE) BY FLOW CYTOMETRY

2007 ◽  
Vol 76 (3) ◽  
pp. 516-521 ◽  
Author(s):  
FRANCISCO PANZERA ◽  
RUBEN PÉREZ ◽  
JANINE RAMSEY ◽  
JOSÉ E. O’CONNOR ◽  
PAZ M. SALAZAR-SCHETTINO ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Genome Size ◽  
Chagas Disease ◽  
Size Determination

scholarly journals Genome size determination of Eclipta alba and Aloe barbadensis

2015 ◽  
Vol 10 (3) ◽  
pp. 697
Author(s):  
Anggana Roy ◽  
Yasir Bashir ◽  
Irfan Ahmad Rather ◽  
Bolin Kumar Konwar
Keyword(s):  
Genetic Diversity ◽  
Flow Cytometry ◽  
Genome Size ◽  
Nuclear Dna ◽  
Genetic Material ◽  
Size Determination ◽  
Reference Standard ◽  
Aloe Barbadensis ◽  
Eclipta Alba

<p class="Abstract">There is abundant genetic diversity of pharmacologically important plants around the globe and this pool of genetic variation serves as the base for selection as well as for plant improvement. The major cause of such genetic diversity is the variation in their genetic material, as called genome. In the present study, an attempt was made to determine the genome size of <em>Eclipta alba</em> and <em>Aloe barbadensis</em> by flow cytometry using Pisum sativum as a reference standard. The nuclear DNA was calculated as 8.7 pg for <em>E. alba</em> and 9.0 pg for <em>A. barbadensis</em>. The genome size of <em>E. alba</em> and <em>A. barbadensis</em> was estimated to be 4.27 x 10<sup>9</sup> bp and 4.42 x 10<sup>9</sup> bp, respectively. Information on genome size and DNA content of these two pharmacologically important plants would provide a useful tool for future molecular biological investigations.</p><p> </p>

First estimates of genome size in ribbon worms (phylum Nemertea) using flow cytometry and Feulgen image analysis densitometry

2014 ◽  
Vol 92 (10) ◽  
pp. 847-851 ◽  
Author(s):  
Kelly L. Mulligan ◽  
Terra C. Hiebert ◽  
Nicholas W. Jeffery ◽  
T. Ryan Gregory
Keyword(s):  
Flow Cytometry ◽  
Image Analysis ◽  
Body Size ◽  
Genome Size ◽  
Positive Relationship ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Size Estimation ◽  
Size Diversity ◽  
Size Estimates

Ribbon worms (phylum Nemertea) are among several animal groups that have been overlooked in past studies of genome-size diversity. Here, we report genome-size estimates for eight species of nemerteans, including representatives of the major lineages in the phylum. Genome sizes in these species ranged more than fivefold, and there was some indication of a positive relationship with body size. Somatic endopolyploidy also appears to be common in these animals. Importantly, this study demonstrates that both of the most common methods of genome-size estimation (flow cytometry and Feulgen image analysis densitometry) can be used to assess genome size in ribbon worms, thereby facilitating additional efforts to investigate patterns of variability in nuclear DNA content in this phylum.

Comparative genome size estimation of different life stages of grey mullet, Mugil cephalus Linnaeus, 1758 by flow cytometry

2021 ◽  
Author(s):  
Jani Angel J. Raymond ◽  
Mudagandur Shashi Shekhar ◽  
Vinaya Kumar Katneni ◽  
Ashok Kumar Jangham ◽  
Sudheesh Kommu Prabhudas ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Genome Size ◽  
Mugil Cephalus ◽  
Life Stages ◽  
Size Estimation ◽  
Grey Mullet ◽  
Comparative Genome

scholarly journals Coconut genome size determined by flow cytometry: Tall versus Dwarf types

2016 ◽  
Vol 15 (1) ◽  
Author(s):  
M. Freitas Neto ◽  
T.N.S. Pereira ◽  
I.G.C. Geronimo ◽  
A.O.N. Azevedo ◽  
S.R.R. Ramos ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Genome Size
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