scholarly journals Coconut genome size determined by flow cytometry: Tall versus Dwarf types

2016 ◽  
Vol 15 (1) ◽  
Author(s):  
M. Freitas Neto ◽  
T.N.S. Pereira ◽  
I.G.C. Geronimo ◽  
A.O.N. Azevedo ◽  
S.R.R. Ramos ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Genome Size

First estimates of genome size in ribbon worms (phylum Nemertea) using flow cytometry and Feulgen image analysis densitometry

2014 ◽  
Vol 92 (10) ◽  
pp. 847-851 ◽  
Author(s):  
Kelly L. Mulligan ◽  
Terra C. Hiebert ◽  
Nicholas W. Jeffery ◽  
T. Ryan Gregory
Keyword(s):  
Flow Cytometry ◽  
Image Analysis ◽  
Body Size ◽  
Genome Size ◽  
Positive Relationship ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Size Estimation ◽  
Size Diversity ◽  
Size Estimates

Ribbon worms (phylum Nemertea) are among several animal groups that have been overlooked in past studies of genome-size diversity. Here, we report genome-size estimates for eight species of nemerteans, including representatives of the major lineages in the phylum. Genome sizes in these species ranged more than fivefold, and there was some indication of a positive relationship with body size. Somatic endopolyploidy also appears to be common in these animals. Importantly, this study demonstrates that both of the most common methods of genome-size estimation (flow cytometry and Feulgen image analysis densitometry) can be used to assess genome size in ribbon worms, thereby facilitating additional efforts to investigate patterns of variability in nuclear DNA content in this phylum.

Comparative genome size estimation of different life stages of grey mullet, Mugil cephalus Linnaeus, 1758 by flow cytometry

2021 ◽  
Author(s):  
Jani Angel J. Raymond ◽  
Mudagandur Shashi Shekhar ◽  
Vinaya Kumar Katneni ◽  
Ashok Kumar Jangham ◽  
Sudheesh Kommu Prabhudas ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Genome Size ◽  
Mugil Cephalus ◽  
Life Stages ◽  
Size Estimation ◽  
Grey Mullet ◽  
Comparative Genome

scholarly journals GENOME SIZE DETERMINATION OF CUCUMBER (CUCUMIS SATIVUS), HONEYDEW (CUCUMIS MELO INODORUS) AND ROCK MELON (CUCUMIS MELO CANTALUPENSIS) VIA FLOW CYTOMETRY

2021 ◽  
Vol 5 (1) ◽  
pp. 14-16
Author(s):  
Raden Muhamad Imaduddin Yumni ◽  
Mohd Fauzihan Karim ◽  
Mohd Razik Midin
Keyword(s):  
Flow Cytometry ◽  
Genome Size ◽  
Cucumis Melo ◽  
As Species ◽  
External Reference ◽  
The Family ◽  
Cucumis Species ◽  
Fcm Analysis ◽  
Interspecific And Intraspecific Variation

The family of Cucurbitaceae consists of species with economical and nutritional value. Morphologically, there are only few differences between Cucumis species. The interspecific and intraspecific variation in the genome size of the Cucumis species are not discovered yet. Due to this, this study aims to determine the genome size of C. sativus, C. melo inodorus and C. melo cantalupensis using flow cytometry (FCM) method. Nuclei suspension of selected Cucumis species were extracted using LBO1 lysis buffer by manual chopping technique and stained by propidium iodide priot to FCM analysis. Genome size of C. sativus, C. melo inodorus (Honeydew) and C. melo cantalupensis (Rockmelon) were determined by using Glycine max (Soybean) as an external reference standard (2C = 2.5 pg). This study found that the genome size of C. sativus, C. melo inodorus and C. melo cantalupensis estimated to be 2.83 pg, 3.00 pg and 3.47 pg respectively. The genome size data obtained from this study can be used in future genome studies as well as species characterization.

scholarly journals Ploidy, Relative Genome Size, and Inheritance of Spotted Foliage in Aucuba Species (Garryaceae)

HortScience ◽  
2018 ◽  
Vol 53 (9) ◽  
pp. 1271-1274
Author(s):  
Thomas G. Ranney ◽  
Tracy H. Thomasson ◽  
Kristin Neill ◽  
Nathan P. Lynch ◽  
Mark Weathington
Keyword(s):  
Flow Cytometry ◽  
Genome Size ◽  
Ploidy Level ◽  
Nuclear Gene ◽  
Discrete Groups ◽  
Basic Information ◽  
Chromosome Counts ◽  
Leaf Trait ◽  
Leaf Variegation ◽  
Evergreen Shrubs

Aucuba have been cultivated for centuries and are valued as adaptable, broad-leaved, evergreen shrubs that also can have attractive, spotted variegations on the foliage. Improved understanding of the cytogenetics and heritability of specific traits, for specific clones and cultivars, can provide basic information to help facilitate the breeding and improvement of aucuba. The objectives of this study were to determine ploidy level and relative genome size of a diverse collection of species and cultivars of aucuba using flow cytometry and cytology and to make additional observations on heritability of spotted leaf variegation. Chromosome counts were 2n = 2x = 16 for Aucuba chinensis (A. omeiensis), 2n = 4x = 32 for A. japonica ‘Rozannie’, and 2n = 6x = 48 for A. sp. ‘Hosoba’. Relative 2C genome size for the 57 taxa varied from 13.8 pg for A. obcordata to 42.0 pg for A. ‘Hosoba’ and fell within three discrete groups consistent with cytotype. Genome size for diploid taxa (A. chinensis and A. obcordata) ranged from 13.8 to 21.0 pg, tetraploids (A. himalaica var. oblanceolata, A. japonica, and A. japonica var. borealis) ranged from 28.8 to 31.2 pg, and the first-ever reported hexaploids (A. ‘Hosoba’ and A. sp. – Vietnam) ranged from 40.5 to 42.0 pg. Unlike prior reports that indicated inheritance of spotted variegations were extranuclear genes that were maternally inherited, we found that the spotted leaf trait expressed in A. japonica ‘Shilpot’ appears to be a nuclear gene that is inherited in a quantitative fashion and not strictly maternal. These data provide an enhanced foundation for breeding improved aucuba.

scholarly journals Flying High—Muscle-Specific Underreplication in Drosophila

Genes ◽  
2020 ◽  
Vol 11 (3) ◽  
pp. 246 ◽  
Author(s):  
J. Spencer Johnston ◽  
Mary E. Zapalac ◽  
Carl E. Hjelmen
Keyword(s):  
Flow Cytometry ◽  
Dna Synthesis ◽  
Salivary Glands ◽  
Genome Size ◽  
Flight Muscle ◽  
S Phase ◽  
The Other ◽  
Tissue Type ◽  
Flight Muscles ◽  
Longitudinal Muscles

Drosophila underreplicate the DNA of thoracic nuclei, stalling during S phase at a point that is proportional to the total genome size in each species. In polytene tissues, such as the Drosophila salivary glands, all of the nuclei initiate multiple rounds of DNA synthesis and underreplicate. Yet, only half of the nuclei isolated from the thorax stall; the other half do not initiate S phase. Our question was, why half? To address this question, we use flow cytometry to compare underreplication phenotypes between thoracic tissues. When individual thoracic tissues are dissected and the proportion of stalled DNA synthesis is scored in each tissue type, we find that underreplication occurs in the indirect flight muscle, with the majority of underreplicated nuclei in the dorsal longitudinal muscles (DLM). Half of the DNA in the DLM nuclei stall at S phase between the unreplicated G0 and fully replicated G1. The dorsal ventral flight muscle provides the other source of underreplication, and yet, there, the replication stall point is earlier (less DNA replicated), and the endocycle is initiated. The differences in underreplication and ploidy in the indirect flight muscles provide a new tool to study heterochromatin, underreplication and endocycle control.

Genome-size variation in bivalve molluscs determined by flow cytometry

1996 ◽  
Vol 126 (3) ◽  
pp. 489-497 ◽  
Author(s):  
A. M. Rodr�guez-Ju�z ◽  
M. Torrado ◽  
J. M�ndez
Keyword(s):  
Flow Cytometry ◽  
Genome Size ◽  
Size Variation ◽  
Bivalve Molluscs ◽  
Genome Size Variation

The genome sizes of Hordeum species show considerable variation

Genome ◽  
1996 ◽  
Vol 39 (4) ◽  
pp. 730-735 ◽  
Author(s):  
Juha Kankanpää ◽  
Alan H. Schulman ◽  
Leena Mannonen
Keyword(s):  
Flow Cytometry ◽  
Hordeum Vulgare ◽  
Genome Size ◽  
Nuclear Dna ◽  
Size Variation ◽  
Nuclear Dna Content ◽  
Meiotic Pairing ◽  
Genome Size Variation ◽  
Dapi Staining ◽  
Hordeum Vulgare Ssp

Hordeum, distributed worldwide in temperate zones, is the second largest genus in the tribe Triticeae and includes diploid, tetraploid, and hexaploid species. We determined, by DAPI staining and flow cytometry, the nuclear DNA content for 35 accessions of the genus Hordeum, from a total of 19 species, including specimens of 2 cultivars and 2 landraces of Hordeum vulgare ssp. vulgare as well as samples of 12 Hordeum vulgare ssp. spontaneum populations. Genome sizes ranged from 5.69 to 9.41 pg for the G1 nuclei of the diploids, and from 13.13 to 18.36 pg for those of the tetraploids. This constitutes a 1.7-fold variation for the diploids, contrasting with a 4% variation previously reported. For H. vulgare ssp. vulgare (barley), the accessions examined differed by 18%. These variations in genome size cannot be correlated with meiotic pairing groups (I, H, X, Y) or with proposed phylogenetic relationships within the genus. Genome size variation between barley accessions cannot be related to status as cultivated or wild, or to climatic or geological gradients. We suggest these data may indicate rapid but sporadic changes in genome size within the genus. Key words : barley, Hordeum, Triticeae, genome size, flow cytometry.

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