Nuclear Genome Size Determination Of Christia Vespertilionis Via Flow Cytometry

AbstractChristia vespertilionis (butterfly wing plant) is an ornamental plant originated from South East Asia with reported usage in traditional medicine practice and potential as an anticancer and antitumor. This research aims to estimate the genome size of C. vespertilionis via flow cytometry (FCM) method. The research was conducted with the optimisation of nuclear suspension preparation followed by the genome size estimation. Two chopping techniques [manual chopping (MC) and BDTM Medimachine (MM)] and two lysis buffers (Otto and LBO1) were tested. Otto buffer with manual chopping was found to be the most suitable method, generated fine DNA peak with minimum debris background, and coefficient of variation (CV) value less than 3%. Five replicates of the FCM analysis were made for the genome size determination. The estimated genome size of C. vespertilionis was found to be 3.22 pg by using Glycine max cv. Polanka (2C=2.5pg) as an external reference standard. Further comparison with other Christia species was not possible due to the lack of data on genome size. The genome size data of C. vespertilionis can be useful for future morphology and genetics studies of Christia species.

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GENOME SIZE DETERMINATION OF CUCUMBER (CUCUMIS SATIVUS), HONEYDEW (CUCUMIS MELO INODORUS) AND ROCK MELON (CUCUMIS MELO CANTALUPENSIS) VIA FLOW CYTOMETRY

Science Heritage Journal ◽

10.26480/gws.01.2021.14.16 ◽

2021 ◽

Vol 5 (1) ◽

pp. 14-16

Author(s):

Raden Muhamad Imaduddin Yumni ◽

Mohd Fauzihan Karim ◽

Mohd Razik Midin

Keyword(s):

Flow Cytometry ◽

Genome Size ◽

Cucumis Melo ◽

As Species ◽

External Reference ◽

The Family ◽

Cucumis Species ◽

Fcm Analysis ◽

Interspecific And Intraspecific Variation

The family of Cucurbitaceae consists of species with economical and nutritional value. Morphologically, there are only few differences between Cucumis species. The interspecific and intraspecific variation in the genome size of the Cucumis species are not discovered yet. Due to this, this study aims to determine the genome size of C. sativus, C. melo inodorus and C. melo cantalupensis using flow cytometry (FCM) method. Nuclei suspension of selected Cucumis species were extracted using LBO1 lysis buffer by manual chopping technique and stained by propidium iodide priot to FCM analysis. Genome size of C. sativus, C. melo inodorus (Honeydew) and C. melo cantalupensis (Rockmelon) were determined by using Glycine max (Soybean) as an external reference standard (2C = 2.5 pg). This study found that the genome size of C. sativus, C. melo inodorus and C. melo cantalupensis estimated to be 2.83 pg, 3.00 pg and 3.47 pg respectively. The genome size data obtained from this study can be used in future genome studies as well as species characterization.

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Cytogenetic relationships within the Maghrebian clade of Festuca subgen. Schedonorus (Poaceae), using flow cytometry and FISH

Anales del Jardín Botánico de Madrid ◽

10.3989/ajbm.2455 ◽

2017 ◽

Vol 74 (1) ◽

pp. 052 ◽

Cited By ~ 3

Author(s):

David Ezquerro-López ◽

David Kopecký ◽

Luis Á. Inda

Keyword(s):

Flow Cytometry ◽

Genome Size ◽

Fluorescent In Situ Hybridization ◽

Genome Duplication ◽

Size Estimation ◽

Ploidy Levels ◽

New Information ◽

Shed Light

Festuca subgen. Schedonorus is a group of broad-leaved fescues, which can be divided into two clades: European and Maghrebian. We employed fluorescent in situ hybridization —FISH— with probes specific for 5S and 35S ribosomal DNA and genome size estimation using flow cytometry to shed light on the determination of possible parental genomes of polyploid species of the Maghrebian clade. Our results indicate that octoploid F. arundinacea subsp. atlantigena probably originated from crossing of the tetraploids F. arundinacea subsp. fenas —2n = 4x = 28— and F. mairei —2n = 4x = 28— followed by whole genome duplication. However, a large reconstruction of karyotype and genome downsizing has been revealed. Similarly, hexaploid F. arundinacea subsp. corsica presumably resulted from the interspecific hybridization of the diploid F. pratensis and tetraploid F. arundinacea subsp. fenas. Several scenarios on the origin of decaploid F. arundinacea var. letourneuxiana are discussed. This study contributed to our knowledge on the phylogeny of broad-leaved fescues and provided new information on the karyotypes —chromosome numbers, ploidy levels and numbers and positions of rDNA loci— using FISH and genome size estimations using flow cytometry in selected taxa of this important grass genus.

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Genome size determination of Eclipta alba and Aloe barbadensis

Bangladesh Journal of Pharmacology ◽

10.3329/bjp.v10i3.23642 ◽

2015 ◽

Vol 10 (3) ◽

pp. 697

Author(s):

Anggana Roy ◽

Yasir Bashir ◽

Irfan Ahmad Rather ◽

Bolin Kumar Konwar

Keyword(s):

Genetic Diversity ◽

Flow Cytometry ◽

Genome Size ◽

Nuclear Dna ◽

Genetic Material ◽

Size Determination ◽

Reference Standard ◽

Aloe Barbadensis ◽

Eclipta Alba

There is abundant genetic diversity of pharmacologically important plants around the globe and this pool of genetic variation serves as the base for selection as well as for plant improvement. The major cause of such genetic diversity is the variation in their genetic material, as called genome. In the present study, an attempt was made to determine the genome size of Eclipta alba and Aloe barbadensis by flow cytometry using Pisum sativum as a reference standard. The nuclear DNA was calculated as 8.7 pg for E. alba and 9.0 pg for A. barbadensis. The genome size of E. alba and A. barbadensis was estimated to be 4.27 x 109 bp and 4.42 x 109 bp, respectively. Information on genome size and DNA content of these two pharmacologically important plants would provide a useful tool for future molecular biological investigations.

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First estimates of genome size in ribbon worms (phylum Nemertea) using flow cytometry and Feulgen image analysis densitometry

Canadian Journal of Zoology ◽

10.1139/cjz-2014-0068 ◽

2014 ◽

Vol 92 (10) ◽

pp. 847-851 ◽

Cited By ~ 3

Author(s):

Kelly L. Mulligan ◽

Terra C. Hiebert ◽

Nicholas W. Jeffery ◽

T. Ryan Gregory

Keyword(s):

Flow Cytometry ◽

Image Analysis ◽

Body Size ◽

Genome Size ◽

Positive Relationship ◽

Nuclear Dna ◽

Nuclear Dna Content ◽

Size Estimation ◽

Size Diversity ◽

Size Estimates

Ribbon worms (phylum Nemertea) are among several animal groups that have been overlooked in past studies of genome-size diversity. Here, we report genome-size estimates for eight species of nemerteans, including representatives of the major lineages in the phylum. Genome sizes in these species ranged more than fivefold, and there was some indication of a positive relationship with body size. Somatic endopolyploidy also appears to be common in these animals. Importantly, this study demonstrates that both of the most common methods of genome-size estimation (flow cytometry and Feulgen image analysis densitometry) can be used to assess genome size in ribbon worms, thereby facilitating additional efforts to investigate patterns of variability in nuclear DNA content in this phylum.

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