Fluorination in the Design of Membrane Protein Assemblies

2013 ◽  
pp. 227-243
Author(s):  
Vijay M. Krishnamurthy ◽  
Krishna Kumar
Keyword(s):  
Membrane Protein ◽  
Protein Assemblies

scholarly journals Predictive energy landscapes for folding membrane protein assemblies

2015 ◽  
Vol 143 (24) ◽  
pp. 243101 ◽  
Author(s):  
Ha H. Truong ◽  
Bobby L. Kim ◽  
Nicholas P. Schafer ◽  
Peter G. Wolynes
Keyword(s):  
Membrane Protein ◽  
Energy Landscapes ◽  
Protein Assemblies

scholarly journals The Peptidisc, a simple method for stabilizing membrane proteins in detergent-free solution

eLife ◽  
2018 ◽  
Vol 7 ◽  
Author(s):  
Michael Luke Carlson ◽  
John William Young ◽  
Zhiyu Zhao ◽  
Lucien Fabre ◽  
Daniel Jun ◽  
...  
Keyword(s):  
Membrane Proteins ◽  
Membrane Protein ◽  
Cost Effective ◽  
Water Soluble ◽  
Protein Assemblies ◽  
Simple Method ◽  
Helical Peptide ◽  
Biological Studies ◽  
Multiple Copies ◽  
Exposed Part

Membrane proteins are difficult to work with due to their insolubility in aqueous solution and quite often their poor stability in detergent micelles. Here, we present the peptidisc for their facile capture into water-soluble particles. Unlike the nanodisc, which requires scaffold proteins of different lengths and precise amounts of matching lipids, reconstitution of detergent solubilized proteins in peptidisc only requires a short amphipathic bi-helical peptide (NSPr) and no extra lipids. Multiple copies of the peptide wrap around to shield the membrane-exposed part of the target protein. We demonstrate the effectiveness of this ‘one size fits all’ method using five different membrane protein assemblies (MalFGK2, FhuA, SecYEG, OmpF, BRC) during ‘on-column’, ‘in-gel’, and ‘on-bead’ reconstitution embedded within the membrane protein purification protocol. The peptidisc method is rapid and cost-effective, and it may emerge as a universal tool for high-throughput stabilization of membrane proteins to advance modern biological studies.

scholarly journals Structural biology of endogenous membrane protein assemblies in native nanodiscs

2021 ◽  
Vol 69 ◽  
pp. 70-77
Author(s):  
Chanelle J. Brown ◽  
Catharine Trieber ◽  
Michael Overduin
Keyword(s):  
Membrane Protein ◽  
Structural Biology ◽  
Protein Assemblies

Neutron Reflectometry of Membrane Protein Assemblies at the Solid/Liquid Interface

2005 ◽  
Vol 58 (9) ◽  
pp. 674 ◽  
Author(s):  
Stephen A. Holt ◽  
Jeremy H. Lakey ◽  
Sofian M. Daud ◽  
Neil Keegan
Keyword(s):  
Quantitative Analysis ◽  
Membrane Protein ◽  
Self Assembly ◽  
Neutron Reflectometry ◽  
The Self ◽  
Water Molecules ◽  
Gold Substrate ◽  
Protein Assemblies ◽  
Solid Liquid Interface ◽  
Solid Liquid

Neutron reflectometry has been used to study the self-assembly of a membrane protein, OmpF, onto a gold-coated silicon substrate from solution. OmpF associates into trimers and has been modified so that each trimer binds to the gold substrate through cysteine residues. Quantitative analysis of the data revealed that 12% of the surface was covered by the oriented protein with 27600 water molecules surrounding each trimer.

scholarly journals Infrared Laser Activation of Soluble and Membrane Protein Assemblies in the Gas Phase

2016 ◽  
Vol 88 (14) ◽  
pp. 7060-7067 ◽  
Author(s):  
Victor A. Mikhailov ◽  
Idlir Liko ◽  
Todd H. Mize ◽  
Matthew F. Bush ◽  
Justin L. P. Benesch ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Gas Phase ◽  
Infrared Laser ◽  
Protein Assemblies
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