Peptide Affinity Chromatography Based on Combinatorial Strategies for Protein Purification

2014 ◽  
pp. 277-302 ◽  
Author(s):  
Silvia Andrea Camperi ◽  
María Camila Martínez-Ceron ◽  
Silvana Laura Giudicessi ◽  
Mariela Mirta Marani ◽  
Fernando Albericio ◽  
...  
Keyword(s):  
Affinity Chromatography ◽  
Protein Purification ◽  
Peptide Affinity

Peptide affinity chromatography of human clotting factor VIII

1998 ◽  
Vol 715 (1) ◽  
pp. 191-201 ◽  
Author(s):  
Roman Necina ◽  
Karin Amatschek ◽  
Eva Schallaun ◽  
Horst Schwinn ◽  
Djuro Josic ◽  
...  
Keyword(s):  
Factor Viii ◽  
Affinity Chromatography ◽  
Clotting Factor ◽  
Peptide Affinity ◽  
Human Clotting Factor

scholarly journals Peptide Affinity Chromatography Applied to Therapeutic Antibodies Purification

2021 ◽  
Author(s):  
Gabriela R. Barredo-Vacchelli ◽  
Silvana L. Giudicessi ◽  
María C. Martínez-Ceron ◽  
Osvaldo Cascone ◽  
Silvia A. Camperi
Keyword(s):  
Affinity Chromatography ◽  
Therapeutic Antibodies ◽  
Peptide Affinity

scholarly journals Bevacizumab Purification by Peptide Affinity Chromatography

2018 ◽  
Author(s):  
Gabriela R. Barredo ◽  
Silvana L. Giudicessi ◽  
Mara C. Martnez-Ceron ◽  
Soledad L. Saavedra ◽  
Gustavo Mahler ◽  
...  
Keyword(s):  
Affinity Chromatography ◽  
Peptide Affinity

scholarly journals Highly selective magnetic affinity purification of histidine-tagged proteins by Ni2+ carrying monodisperse composite microspheres

RSC Advances ◽  
2017 ◽  
Vol 7 (14) ◽  
pp. 8718-8726 ◽  
Author(s):  
Kouroush Salimi ◽  
Duygu Deniz Usta ◽  
İlkay Koçer ◽  
Eda Çelik ◽  
Ali Tuncel
Keyword(s):  
Affinity Chromatography ◽  
Protein Purification ◽  
Affinity Purification ◽  
Porous Silica ◽  
Immobilized Metal Affinity Chromatography ◽  
Silica Microspheres ◽  
Magnetic Sorbent ◽  
Metal Affinity ◽  
Composite Microspheres

A magnetic sorbent based on monodisperse-porous silica microspheres was developed for His-tagged protein purification by immobilized metal affinity chromatography.

scholarly journals pAM401-Based Shuttle Vectors That Enable Overexpression of Promoterless Genes and One-Step Purification of Tag Fusion Proteins Directly from Enterococcus faecalis

2001 ◽  
Vol 67 (3) ◽  
pp. 1262-1267 ◽  
Author(s):  
Shuhei Fujimoto ◽  
Yasuyoshi Ike
Keyword(s):  
Affinity Chromatography ◽  
Protein Purification ◽  
Enterococcus Faecalis ◽  
Stop Codon ◽  
Initiation Site ◽  
Translation Initiation Site ◽  
Chromatography Method ◽  
Shuttle Vectors ◽  
E Coli ◽  
One Step

ABSTRACT Two novel Enterococcus faecalis-Escherichia colishuttle vectors that utilize the promoter and ribosome binding site ofbacA on the E. faecalis plasmid pPD1 were constructed. The vectors were named pMGS100 and pMGS101. pMGS100 was designed to overexpress cloned genes in E. coli andE. faecalis and encodes the bacA promoter followed by a cloning site and stop codon. pMGS101 was designed for the overexpression and purification of a cloned protein fused to a Strep-tag consisting of 9 amino acids at the carboxyl terminus. The Strep-tag provides the cloned protein with an affinity to immobilized streptavidin that facilitates protein purification. We cloned a promoterless β-galactosidase gene from E. coli and cloned the traA gene of the E. faecalis plasmid pAD1 into the vectors to test gene expression and protein purification, respectively. β-Galactosidase was expressed in E. coliand E. faecalis at levels of 103 and 10 Miller units, respectively. By cloning the pAD1 traA into pMGS101, the protein could be purified directly from a crude lysate of E. faecalis or E. coli with an immobilized streptavidin matrix by one-step affinity chromatography. The ability of TraA to bind DNA was demonstrated by the DNA-associated protein tag affinity chromatography method using lysates prepared from both E. coli and E. faecalis that overexpress TraA. The results demonstrated the usefulness of the vectors for the overexpression and cis/trans analysis of regulatory genes, purification and copurification of proteins from E. faecalis, DNA binding analysis, determination of translation initiation site, and other applications that require proteins purified from E. faecalis.

scholarly journals Current Trends in Protein Purification : A Review

2020 ◽  
pp. 279-310
Author(s):  
Angela Boxi ◽  
Isha Parikh ◽  
Radhika B S ◽  
Shryli K S
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
Ion Exchange ◽  
Affinity Chromatography ◽  
Protein Purification ◽  
Gel Filtration ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Current Trends ◽  
Comparative Information

The present review is based on papers published between 1990 and 2020 and gives Comparative information about the most common protein purification techniques Gel-Filtration, Chromatography, Ion-Exchange Chromatography, Electrophoresis, Affinity Chromatography, and Dialysis, High-Pressure Liquid Chromatography. and their applications.

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