FITC-Labelled Clone from Phage Display for Direct Detection of Leukemia Cells in Blood

Development of sugar chain-binding single-chain variable fragment antibody to adult T-cell leukemia cells using glyco-nanotechnology and phage display method

The Journal of Biochemistry ◽

10.1093/jb/mvy005 ◽

2018 ◽

Vol 163 (4) ◽

pp. 281-291

Author(s):

Kaname Muchima ◽

Taro Todaka ◽

Hiroyuki Shinchi ◽

Ayaka Sato ◽

Arisa Tazoe ◽

...

Keyword(s):

T Cell ◽

Phage Display ◽

Leukemia Cells ◽

T Cell Leukemia ◽

Single Chain Variable Fragment ◽

Cell Leukemia ◽

Single Chain ◽

Sugar Chain ◽

Adult T Cell Leukemia

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Detection and Characterization of earth-like Planets

International Astronomical Union Colloquium ◽

10.1017/s0252921100014810 ◽

1997 ◽

Vol 161 ◽

pp. 299-311 ◽

Cited By ~ 22

Author(s):

Jean Marie Mariotti ◽

Alain Léger ◽

Bertrand Mennesson ◽

Marc Ollivier

Keyword(s):

Direct Detection ◽

Contrast Ratio ◽

Angular Size ◽

Physical Nature ◽

Major Axis ◽

Infrared Emission ◽

Space Technology ◽

Semi Major Axis ◽

Earth Like Planets ◽

Visible Wavelengths

AbstractIndirect methods of detection of exo-planets (by radial velocity, astrometry, occultations,...) have revealed recently the first cases of exo-planets, and will in the near future expand our knowledge of these systems. They will provide statistical informations on the dynamical parameters: semi-major axis, eccentricities, inclinations,... But the physical nature of these planets will remain mostly unknown. Only for the larger ones (exo-Jupiters), an estimate of the mass will be accessible. To characterize in more details Earth-like exo-planets, direct detection (i.e., direct observation of photons from the planet) is required. This is a much more challenging observational program. The exo-planets are extremely faint with respect to their star: the contrast ratio is about 10−10at visible wavelengths. Also the angular size of the apparent orbit is small, typically 0.1 second of arc. While the first point calls for observations in the infrared (where the contrast goes up to 10−7) and with a coronograph, the latter implies using an interferometer. Several space projects combining these techniques have been recently proposed. They aim at surveying a few hundreds of nearby single solar-like stars in search for Earth-like planets, and at performing a low resolution spectroscopic analysis of their infrared emission in order to reveal the presence in the atmosphere of the planet of CO H2O and O3. The latter is a good tracer of the presence of oxygen which could be, like on our Earth, released by biological activity. Although extremely ambitious, these projects could be realized using space technology either already available or in development for others missions. They could be built and launched during the first decades on the next century.

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Ferritin-Conjugated Antibody for the Demonstration of Isoantigens on the Surface of Murine Cells

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100099982 ◽

1968 ◽

Vol 26 ◽

pp. 48-49

Author(s):

T. Aoki ◽

J. Izard ◽

U. Hämmerling ◽

E. de Harven ◽

L. J. Old

Keyword(s):

Cell Surface ◽

Gamma Globulin ◽

Leukemia Cells ◽

Labelling Technique ◽

Direct Labelling ◽

Labelling Method

Although a variety of viral and cellular antigens have been demonstrated by ferritin-labeled antibody, this technique has not been used to locate isoantigens on the surface of nucleated cells. The recognition of several systems of isoantigens on the surface of thymocytes, lymphocytes and leukemia cells of the mouse and the ease with which these cells can be obtained in free suspension led us to consider the ferritin-labelling method to determine the amount and location of these isoantigens on the cell surface. Because of the problems involved in the direct labelling of mouse gamma globulin by ferritin, we have chosen an indirect labelling technique (i.e. ferritin-conjugated rabbit anti mouse γG)to detect localization of mouse isoantibody.

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The distribution of antigen on the surface of RBL-2H3 rat basophilic leukemia cells observed by back-scattered electron imaging

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100142992 ◽

1986 ◽

Vol 44 ◽

pp. 274-275

Author(s):

R.F. Stump ◽

J.R. Pfeiffer ◽

JC. Seagrave ◽

D. Huskisson ◽

J.M. Oliver

Keyword(s):

Cell Surface ◽

Dynamic Properties ◽

Leukemia Cells ◽

Antigen Binding ◽

Scattered Electron ◽

Ige Receptor ◽

Receptor Complexes ◽

Rat Basophilic Leukemia Cells ◽

Electron Imaging ◽

Basophilic Leukemia

In RBL-2H3 rat basophilic leukemia cells, antigen binding to cell surface IgE-receptor complexes stimulates the release of inflammatory mediators and initiates a series of membrane and cytoskeletal events including a transformation of the cell surface from a microvillous to a lamellar topography. It is likely that dynamic properties of the IgE receptor contribute to the activation of these responses. Fewtrell and Metzger have established that limited crosslinking of IgE-receptor complexes is essential to trigger secretion. In addition, Baird and colleagues have reported that antigen binding causes a rapid immobilization of IgE-receptor complexes, and we have demonstrated an apparent increase with time in the affinity of IgE-receptor complexes for antigen.

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Involvement of GER in Friend leukemia virus assembly in high-pressure frozen cells

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100160509 ◽

1990 ◽

Vol 48 (3) ◽

pp. 590-591

Author(s):

W. Djaczenko ◽

M. Müller ◽

A. Benedetto ◽

G. Carbone

Keyword(s):

High Pressure ◽

Virus Assembly ◽

Leukemia Virus ◽

Leukemia Cells ◽

Serial Sections ◽

Myeloma Cells ◽

Virus Particles ◽

Friend Leukemia ◽

Carbon Coated ◽

Friend Leukemia Virus

A thickening of ER membranes in murine myeloma cells was attributed by de Harven to the assembly of intracisternal virus particles. We observed similar thickening of GER membranes in Friend leukemia cells (FLC) apparently associated with Friend leukemia virus (FLV) assembly. We reinvestigated the problem of GER involvement in FLV assembly using high pressure cryofixed FLC.FLC (745A clone growing in suspension and FF clone growing in monolayer) were immersed in Hexadecene (Fluka, Switzerland) and rapidly frozen in Balzers HPM 010 freezing machine working at 2200 bar. All cells were freeze substituted at -90°C in 2% OsO4 in absolute acetone. Serial sections cut to avoid misinterpretations due to the geometry of sections, were collected on carbon coated 100 mesh grids.

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